scholarly journals Isolation of Neural Stem Cells from Whole Brain Tissues of Adult Mice

2019 ◽  
Vol 49 (1) ◽  
pp. e80
Author(s):  
Krutika Deshpande ◽  
Behnaz Saatian ◽  
Vahan Martirosian ◽  
Michelle Lin ◽  
Alex Julian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Whole Brain ◽  
Adult Mice ◽  
Brain Tissues

Abstract 214: The Vascular Niche in Adult Mouse Brain After Stroke - Confocal Imaging of Whole Mount SVZ

Stroke ◽  
2013 ◽  
Vol 44 (suppl_1) ◽  
Author(s):  
Rui Lan Zhang ◽  
Michael Chopp ◽  
Cynthia Roberts ◽  
Siamak Nejad-Davarani ◽  
Li Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Neural Stem Cells ◽  
Blood Vessels ◽  
Artery Occlusion ◽  
Confocal Imaging ◽  
Adult Mice ◽  
Cell Niche ◽  
Newborn Neurons ◽  
Whole Mounts

Stroke induces angiogenesis in the peri-infarct region. It is not known whether angiogenesis occurs in the subventricular zone (SVZ) of the lateral ventricle after stroke. The SVZ is a neural stem cell niche containing vascular plexus that supports adult neurogenesis. We characterized longitudinal changes of vascular structures in the SVZ niche after stroke using whole mounts, an organotypic preparation of the SVZ in which the 3D cell-vessel relationships are preserved. Adult mice were subjected to middle cerebral artery occlusion (MCAO). The vascular architectures within the 50 μm thick SVZ of immunostained whole mounts were imaged by 3D confocal microscopy. In non-MCAO mice (n=4), 2±0.2% of endothelial cells were proliferative (BrdU+/CD31+). Blood vessels in this niche constituted 2.6±0.01% of the total volume with 75±17 vascular branches. However, 7 and 14 days after MCAO, proliferated endothelial cells significantly (p<0.05) increased to 12±1% (n=5) and 15±1 % (n=5), respectively, which was followed by substantial increases in vascular volume at 14 (4.2±0.01%, n=3), 30 (4.9±0.05%, n=3), and 90 (5.7±0.01%, n=3) days, but not at 7 days after MCAO. Moreover, vascular branches increased significantly to 156±27 and 216±8 at 30 and 90 days, respectively, but not at 14 days. Interestingly, we detected increases in the number of string-like vessels starting at 14 days (731±79/mm 3 vs 476±41/mm 3 in control) which increased and persisted at 30 (1,824±255/mm 3 ) and 90 (1,748±204/mm 3 ) days after MCAO. These string-like vessels were not perfused by plasma. String vessels increase during embryonic angiogenesis. Collectively, these data indicate that stroke induces angiogenesis in the SVZ, which lasts at least 90 days after stroke. Moreover, stroke significantly increased neural stem cells (BrdU + /GFAP + , 13±3%, 15±3%, and 11±4% at 7, 14, and 90 days, respectively, vs 6±1% in control) and newborn neurons (BrdU + /DCX + , 14±2% and 12±2.0% at 7 and 14 days, respectively, vs 4±1% in control). Neural stem cells at the ventricular surface extended their processes to the blood vessels in the SVZ. Our data indicate that stroke induces angiogenesis in the SVZ, which is associated with stroke-induced neurogenesis.

Abstract TP78: Combination of Neuroprotective Drug and Neural Stem Cells Benefits Aged Stroke Mice with Delayed Tissue Plasminogen Activator Treatment

Stroke ◽  
2017 ◽  
Vol 48 (suppl_1) ◽  
Author(s):  
Auston Eckert ◽  
Milton H Hamblin ◽  
Jean-Pyo Lee
Keyword(s):  
Stem Cells ◽  
Young Adult ◽  
Neural Stem Cells ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Inflammatory Drug ◽  
Aged Mice ◽  
Post Stroke ◽  
Adult Mice ◽  
Tissue Plasminogen

Background: Presently, tissue plasminogen activator (tPA) is the sole FDA-approved antithrombotic treatment available for stroke. However, tPA’s harmful side effects within the central nervous system can exacerbate blood-brain barrier (BBB) damage and increase mortality. Patients should receive tPA less than 4.5 hours post-stroke. Although age alone is not an impediment for tPA treatment, the harmful effects of delayed tPA (>4.5h), particularly on aged stroke animals, have not been well studied. We reported that intracranial transplantation of neural stem cells (hNSCs) ameliorates BBB damage caused by ischemic stroke. In this study, we examined the combined effects of minocycline (a neuroprotective and anti-inflammatory drug) and hNSC transplantation on the mortality of delayed tPA-treated aged mice within 48h post-stroke. Methods and Results: We utilized the middle cerebral artery occlusion stroke mouse model to induce focal cerebral ischemia followed by reperfusion (MCAO/R). 6h post-MCAO, we administered tPA intravenously. Minocycline was administered intraperitoneally at various time points prior to tPA injection. One day post-stroke, we injected hNSCs intracranially. Previously, we reported that hNSCs (both human and mouse) transplanted into the brain 24h post-stroke rapidly improve neurological outcome in young-adult mice (4-5mo). In our current study, tPA administered within 4.5h did not increase mortality in either young-adult or aged mice. However, we found delayed tPA treatment (6h post-stroke) significantly increased the mortality of aged mice (13-18 mo) but not in young-adult mice. Here, we report that by combining minocycline prior to tPA significantly reduced mortality. Furthermore, transplanting hNSCs in minocycline-treated mice further ameliorated the pathophysiology caused by delayed tPA. Conclusions: Our findings implicate that administering the anti-apototic and anti-inflammatory drug prior to tPA injection, and then post-treating with multipotent neuroprotective hNSCs might expand the time window of tPA and reduce reperfusion injury.

Intravenously transplanted human neural stem cells migrate to the injured spinal cord in adult mice in an SDF-1- and HGF-dependent manner

2007 ◽  
Vol 426 (2) ◽  
pp. 69-74 ◽  
Author(s):  
Hiroki Takeuchi ◽  
Atsushi Natsume ◽  
Toshihiko Wakabayashi ◽  
Chihiro Aoshima ◽  
Shinji Shimato ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Dependent Manner ◽  
Injured Spinal Cord ◽  
Human Neural Stem Cells ◽  
Adult Mice

Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury

2013 ◽  
Vol 19 (2) ◽  
pp. 132-136 ◽  
Author(s):  
Yi-min Zhang ◽  
Yu-qing Zhang ◽  
Shao-bing Cheng ◽  
Sheng-xin Chen ◽  
Ai-lian Chen ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Stem Cells ◽  
Brain Injury ◽  
Neural Stem Cells ◽  
Proliferation And Differentiation ◽  
Brain Tissues

Transplantation of embryonic stem cell-derived neural stem cells for spinal cord injury in adult mice

2005 ◽  
Vol 27 (8) ◽  
pp. 812-819 ◽  
Author(s):  
Hajime Kimura ◽  
Masahide Yoshikawa ◽  
Ryousuke Matsuda ◽  
Hayato Toriumi ◽  
Fumihiko Nishimura ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Adult Mice ◽  
Cord Injury

scholarly journals Response to “In regards to Kirby et al ., Physics strategies for sparing neural stem cells during whole-brain radiation treatments,” [Med. Phys. 38, 5338 (2011)]

2012 ◽  
Vol 39 (3) ◽  
pp. 1679-1679
Author(s):  
Neil Kirby ◽  
Cynthia Chuang ◽  
Jean Pouliot ◽  
Andrew Hwang ◽  
Igor J. Barani
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Whole Brain ◽  
Whole Brain Radiation ◽  
Radiation Treatments ◽  
Brain Radiation
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