Multiple Disease Resistant Lines for Sunflower Breeding

CSA News ◽  
2021 ◽  
Keyword(s):  
Disease Resistant ◽  
Resistant Lines

scholarly journals Development of Molecular Markers Associated with Resistance to Gray Mold Disease in Onion (Allium cepa L.) through RAPD-PCR and Transcriptome Analysis

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 436
Author(s):  
So-Jeong Kim ◽  
Jee-Soo Park ◽  
TaeHoon Park ◽  
Hyun-Min Lee ◽  
Ju-Ri Choi ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Random Amplified Polymorphic Dna ◽  
Gray Mold ◽  
Breeding Period ◽  
Vegetable Crops ◽  
Rna Seq ◽  
Sequence Characterized Amplified Region ◽  
Disease Resistant ◽  
Resistant Lines ◽  
Allium Cepa L

Onions (Allium cepa L.) are one of the most consumed vegetable crops worldwide and are damaged by several fungal diseases in the field or during storage. Gray mold disease caused by the necrotrophic pathogens Botrytis cinerea and Botrytis squamosa is a disease that reduces the productivity and storage life in onions. However, it is difficult to control gray mold disease in onions by using physical and chemical methods. Breeding resistant onions against gray mold disease can reduce the damage caused by pathogens, reduce the labor required for control, and reduce environmental pollution caused by fungicides. However, onions have a large genome size (16Gb), making them difficult to analyze, and have a biennial cycle, resulting in a very long breeding period. Therefore, in this study, markers were developed to shorten the onion breeding period. First, random amplified polymorphic DNA (RAPD) was performed to confirm the genetic relationship between the gray mold disease-resistant and -susceptible lines through a dendrogram. In addition, the sequence characterized amplified region (SCAR)-OPAN1 marker to select resistant lines was developed using a polymorphic RAPD fragment. Second, the RNA-seq of the gray mold-resistant and -susceptible onion lines were analyzed using NGS technology. Using the RNA-seq results and DEG and GO analyses were performed, and the variants, such as SNPs and indels, were analyzed to develop a selectable marker for the resistant line. This study developed the SNP-3 HRM marker for selecting gray mold disease-resistant lines by using the SNPs present in the aldo-keto reductase (AKR) gene with high expression levels in these lines. The SCAR-OPAN1 and SNP-3 HRM markers developed in this study could be used to select gray mold disease-resistant onions in breeding programs to reduce the damage caused by gray mold disease.

scholarly journals Suppressing Effect of Sulfur Materials or Disease-resistant Lines against Southern Blight of Jerusalem Artichoke

2021 ◽  
Vol 20 (2) ◽  
pp. 233-239
Author(s):  
Yuichi Matsumoto ◽  
Syouta Uchida ◽  
Yuka Fukumoto ◽  
Saya Kanaya ◽  
Ayaka Hirano ◽  
...  
Keyword(s):  
Jerusalem Artichoke ◽  
Southern Blight ◽  
Disease Resistant ◽  
Resistant Lines

Genetics, molecular dissection and identification of high yielding disease resistant lines to MYMV in blackgram (Vigna mungo)

2015 ◽  
Vol 38 (6) ◽  
Author(s):  
Kumari Basamma ◽  
P. M. Salimath ◽  
Patil Malagouda ◽  
Biradar Suma
Keyword(s):  
Dominant Gene ◽  
Yellow Mosaic Virus ◽  
Resistant Parent ◽  
Biotic Stresses ◽  
Disease Resistant ◽  
Resistant Lines ◽  
Mungbean Yellow Mosaic Virus ◽  
Artificial Condition ◽  
Major Attention ◽  
Serious Disease

The productivity of blackgram is very low which is mainly due to losses caused by several biotic stresses. Among the biotic stresses mungbean yellow mosaic virus (MYMV) is the most serious disease on this crop with an estimated yield loss of 100% under severe conditions. In this context, study of genetics of resistance to MYMV needs major attention. In this paper, we reported genetics of the resistance to MYMV disease in blackgram using a F<sub>2</sub> and F<sub>3</sub> populations derived from a cross between the susceptible but agronomically superior parent TAU-1 and the resistant parent BDU-4. The parents, F<sub>1</sub>, F<sub>2</sub>, F<sub>3</sub> populations were evaluated for MYMV resistance against the virus in artificial condition. The population size in F<sub>2</sub> was three hundred. The results suggested that the MYMV resistance in blackgram is governed by a single dominant gene. Out of 610 SSR and RGA markers screened 24 were found to be polymorphic between two parents. Based on phenotyping in F<sub>2</sub> and F<sub>3</sub> generations nine high yielding disease resistant lines have been identified.

Evaluation of Papaya Ring Spot Disease Resistant Lines under Western Maharashtra Conditions

2018 ◽  
Vol 7 (special) ◽  
pp. 63
Author(s):  
S U Chalak ◽  
S N Hasabnis ◽  
V S Supe
Keyword(s):  
Spot Disease ◽  
Disease Resistant ◽  
Resistant Lines ◽  
Ring Spot

Effect of Immunostimulation on Healing in Inflammatory Disease-Resistant and Susceptible Rat Strains

2003 ◽  
Author(s):  
Nairmeen Awad Haller ◽  
Benjamin H. Newberry
Keyword(s):  
Inflammatory Disease ◽  
Rat Strains ◽  
Disease Resistant

Radish germplasm with fusarium wilt-resistance as material for breeding disease-resistant varieties

2017 ◽  
Author(s):  
YA Jeon ◽  
JH Rhee ◽  
JS Sung ◽  
HJ Baek ◽  
OS Hur ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Wilt Resistance ◽  
Disease Resistant ◽  
Resistant Varieties ◽  
Fusarium Wilt Resistance

Regeneration System Establishment of Disease-resistant Asparagus Variety

2013 ◽  
Vol 31 (2) ◽  
pp. 158
Author(s):  
Jian-Ming GAO ◽  
Zhen-Zhen DAI ◽  
Feng YANG ◽  
Shi-Qing ZHANG ◽  
He-Long CHEN ◽  
...  
Keyword(s):  
Regeneration System ◽  
Disease Resistant ◽  
System Establishment

Molecular Tools Developed for Disease Resistant Genes in Wheat, Barley, Lentil and Chickpea: AReview

2018 ◽  
Vol 36 (1) ◽  
pp. 050-056 ◽  
Author(s):  
Aladdin Hamawieh ◽  
◽  
Fida Alo ◽  
Seid Ahmed ◽  
◽  
...  
Keyword(s):  
Molecular Tools ◽  
Resistant Genes ◽  
Disease Resistant
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