scholarly journals Development of Molecular Markers Associated with Resistance to Gray Mold Disease in Onion (Allium cepa L.) through RAPD-PCR and Transcriptome Analysis

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 436
Author(s):  
So-Jeong Kim ◽  
Jee-Soo Park ◽  
TaeHoon Park ◽  
Hyun-Min Lee ◽  
Ju-Ri Choi ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Random Amplified Polymorphic Dna ◽  
Gray Mold ◽  
Breeding Period ◽  
Vegetable Crops ◽  
Rna Seq ◽  
Sequence Characterized Amplified Region ◽  
Disease Resistant ◽  
Resistant Lines ◽  
Allium Cepa L

Onions (Allium cepa L.) are one of the most consumed vegetable crops worldwide and are damaged by several fungal diseases in the field or during storage. Gray mold disease caused by the necrotrophic pathogens Botrytis cinerea and Botrytis squamosa is a disease that reduces the productivity and storage life in onions. However, it is difficult to control gray mold disease in onions by using physical and chemical methods. Breeding resistant onions against gray mold disease can reduce the damage caused by pathogens, reduce the labor required for control, and reduce environmental pollution caused by fungicides. However, onions have a large genome size (16Gb), making them difficult to analyze, and have a biennial cycle, resulting in a very long breeding period. Therefore, in this study, markers were developed to shorten the onion breeding period. First, random amplified polymorphic DNA (RAPD) was performed to confirm the genetic relationship between the gray mold disease-resistant and -susceptible lines through a dendrogram. In addition, the sequence characterized amplified region (SCAR)-OPAN1 marker to select resistant lines was developed using a polymorphic RAPD fragment. Second, the RNA-seq of the gray mold-resistant and -susceptible onion lines were analyzed using NGS technology. Using the RNA-seq results and DEG and GO analyses were performed, and the variants, such as SNPs and indels, were analyzed to develop a selectable marker for the resistant line. This study developed the SNP-3 HRM marker for selecting gray mold disease-resistant lines by using the SNPs present in the aldo-keto reductase (AKR) gene with high expression levels in these lines. The SCAR-OPAN1 and SNP-3 HRM markers developed in this study could be used to select gray mold disease-resistant onions in breeding programs to reduce the damage caused by gray mold disease.

scholarly journals Effect of cultural practices on quality and yield of onion (Allium cepa L. Var. Safid e Paisaye)

2020 ◽  
pp. 9-14
Author(s):  
Hamid Salari ◽  
B.S. Hansra ◽  
Yashpal Singh Saharwat
Keyword(s):  
Allium Cepa ◽  
Cultural Practices ◽  
Total Yield ◽  
Planting Date ◽  
Soluble Solids ◽  
Vegetable Crops ◽  
Market Demand ◽  
Marketable Yield ◽  
Land Preparation ◽  
Allium Cepa L

Onion (Allium cepa L.) is among the most cultivated vegetable crops in the world. Afghanistan is thought to be the origin as several local and wild varieties are found in different parts of the country. Safid e Paisaye is a local variety grown in central parts of Afghanistan in the Ghorband valley. This variety has long storability and high market demand among restaurants in the region, but little research has been done to increase the quality and its availability to the market to increase its market share in Afghanistan. Conducted under supervision of Amity University Uttar Pradesh, Noida, India, at Agriculture Faculty Research Farm of Kabul University, this investigation looks at plough depth, land preparation methods, and planting date on quality and yield of onion bulb; it also studied other cultural practices including irrigation and fertilization dose and frequency. The parameters studied in this investigation include neck diameter (cm), bulb diameter (cm), neck to bulb ratio, bulb weight (gr), bulb volume (cm3), bulb density (gr/cm3), Total Soluble Solids (TSS) (Brix), firmness (Kg/cm2), marketable yield (MT/Ha), and total yield (MT/Ha). The data revealed that planting date has significant influence on bulb quality and yield of onion. The highest bulb diameter (6.95 cm), bulb weight (121 gr), bulb volume (128 cm3), marketable yield (32.54 MT/Ha), and total yield (34.24 MT/Ha) and the lowest neck to bulb ratio (0.04) were recorded for the first planting date (seed sown in nursery on 10 March - seedlings planted in field on 10 May). Land ...

scholarly journals Evaluation of the effects of FYM and gypsum on onion (Allium cepa L.) production under sodic water irrigation

2016 ◽  
Vol 8 (4) ◽  
pp. 1878-1883
Author(s):  
Amit Kumar ◽  
A. C. Yadav ◽  
Ansul Ansul ◽  
Vinod Kumar ◽  
Avtar Singh
Keyword(s):  
Allium Cepa ◽  
Poor Quality ◽  
Growth And Yield ◽  
Vegetable Crops ◽  
Seedling Mortality ◽  
Farm Yard Manure ◽  
Sodic Water ◽  
Allium Cepa L ◽  
Minimum Number ◽  
Quality Water

A field experiment was conducted at CCS Haryana Agricultural University, Hisar to study the effect of FYM and gypsum on onion (Allium cepa L.) production under sodic water irrigation. Treatments comprised of three levels of farm yard manure (FYM) (F0- no FYM, F1- 10 t/ha and F2- 20 t/ha FYM) and three levels of gypsum (G0- nogypsum, G1- 50% neutralization of RSC and G2- 100% neutralization of RSC) in addition to control (irrigation with sodic water and no FYM and no gypsum F0G0). Results of the study showed that the seedling mortality was maximum (77.50 %) in control (F0G0) treatment, while minimum number of seedlings mortality (18.17 %) was observed in (F2G2) treatment followed by (25.17 %) (F2G1) treatment. There was significant increase in growth and yield of onion with application of FYM and gypsum. Moreover, maximum bulb yield (180.83 q/ha) was recorded in (F2G2) followed by F2G1 (152.22 q/ha) where 100% sodicity of water was neutralized by the application of gypsum and 20 t/ha FYM. Under sodic water conditions, increasing level of gypsum and FYM help in reducing the sodicity of irrigation water and thereby, increasing the yield of onion. The study concluded that in present situation of scarcity of good quality water for agricultural purposes, use of amendments like FYM and gypsum were best alternative for the best possible use of poor quality ground water and simultaneously sustain the yield of vegetable crops like onion which are sensitive to sodic water.

scholarly journals Genotoxicity of Thermopsis turcica on Allium cepa L. roots revealed by alkaline comet and random amplified polymorphic DNA assays

2014 ◽  
Vol 68 (4) ◽  
pp. 829-838 ◽  
Author(s):  
İbrahim Hakkı Ciğerci ◽  
Süleyman Cenkci ◽  
Mustafa Kargıoğlu ◽  
Muhsin Konuk
Keyword(s):  
Allium Cepa ◽  
Random Amplified Polymorphic Dna ◽  
Allium Cepa L ◽  
Dna Assays ◽  
Thermopsis Turcica

scholarly journals Constraints in Production, Marketing and Processing of Onion (Allium Cepa L.) in Nuh District of Haryana

2020 ◽  
Vol 65 (4) ◽  
Author(s):  
Raj Kumar
Keyword(s):  
Allium Cepa ◽  
Control Measures ◽  
Raw Material ◽  
Primary Data ◽  
Vegetable Crops ◽  
Pests And Diseases ◽  
Marketing Process ◽  
Allium Cepa L ◽  
Technical Manpower ◽  
Lack Of Knowledge

Onion (Allium cepa L.) is one of the important commercial vegetable crops grown in India. The present study was conducted in Nuh district of Haryana on the basis of highest production of onion among other districts of the state. The block named Tauru was selected for sampling and a total of thirty onion farmers from various villages in this block were selected randomly. The primary data for the agriculture year 2017-18 were collected by personal interviews of the selected farmers with the help of specially designed schedule. The results of the study revealed that the major problems faced by the onion farmers in production were observed high cost of pesticide (93.33%), lack of knowledge about recommended fertilizer doses (86.67%), high cost of fertilizer (83.33%), lack of knowledge about the control measures for various pests and diseases (83.33%), difficulty in identifying the pests and diseases (80.00%) and lack of knowledge about seed/seedling treatment (76.67%). As for as marketing of onion is concerned, 83.33 per cent of respondents opined that high cost of transportation, absence of minimum support prices (83.33%), existence of large number of intermediaries in marketing process (83.33%) and too much fluctuation in prices (80.00%). The problems related to processing expressed by onion growers 80.00 per cent of the respondents reported the problem of technical manpower, higher charges of power and fuels (73.33%), fluctuation in raw material and procurement (73.33%) and lack of good quality packaging material (66.67%).

scholarly journals Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)

2017 ◽  
pp. 9-17
Author(s):  
Maja Ignjatov ◽  
Tatjana Popovic ◽  
Dragana Milosevic ◽  
Zorica Nikolic ◽  
Gordana Petrovic ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Elongation Factor ◽  
Molecular Characteristics ◽  
Control Analysis ◽  
Vegetable Crops ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Allium Cepa L ◽  
Onion Seed ◽  
Fusarium Sp

Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen?s morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA se?quence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1? gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3).

scholarly journals Evaluation of Pre-mix Fungicide, Fluopyram and Trifloxystrobin 250SC against Purple Blotch Disease of Onion in Karnataka

2020 ◽  
pp. 44-50
Author(s):  
M. R. Ravikumar ◽  
D. K. Harish ◽  
B. H. Kumara ◽  
Amarendra Kumar
Keyword(s):  
Field Experiment ◽  
Allium Cepa ◽  
Fungal Diseases ◽  
Vegetable Crops ◽  
Allium Cepa L ◽  
Crop Field ◽  
Alternaria Porri ◽  
Serious Disease ◽  
Bulb Yield ◽  
Extensive Damage

Onion (Allium cepa L.) also known as the bulb onion or is rightly called as Queen of kitchen. It is one of the oldest important vegetable crops grown in India. Among the various fungal diseases, purple blotch caused by Alternaria porri is one of the most serious disease of onion which causes extensive damage to bulbs as well as seed crop. Field experiment was carried out to know the effect of combi-fungicide Luna sensation 500SC (Fluopyram250SC and Trifloxystrobin 250SC) at 400, 500 and 600 ml /ha along with recommended fungicides against purple blotch disease of onion during kharif seasons 2017-18 and 2018-19. A total of eight treatments were taken and among them Luna sensation 500SC (Fluopyram250SC and Trifloxystrobin 250SC) 600 ml / ha proved to be best for management of purple blotch 22.03% diseases index (PDI), which was superior over all other treatments with maximum bulb yield of 24.77 t/ha.

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