scholarly journals Cre recombinase activity specific to postnatal, premeiotic male germ cells in transgenic mice

genesis ◽  
2008 ◽  
Vol 46 (12) ◽  
pp. 738-742 ◽  
Author(s):  
Patricia I. Sadate-Ngatchou ◽  
Christopher J. Payne ◽  
Andrea T. Dearth ◽  
Robert E. Braun
Keyword(s):  
Transgenic Mice ◽  
Germ Cells ◽  
Cre Recombinase ◽  
Male Germ Cells

Mutations induced in male germ cells after treatment of transgenic mice with ethylnitrosourea

1997 ◽  
Vol 388 (2-3) ◽  
pp. 229-237 ◽  
Author(s):  
M Katoh ◽  
N Horiya ◽  
R.P.A Valdivia
Keyword(s):  
Transgenic Mice ◽  
Germ Cells ◽  
Male Germ Cells

Ectopic Expression of FIGLA in Male Germ Cells Represses Testis-specific Genes and Disrupts Fertility in Transgenic Mice.

2008 ◽  
Vol 78 (Suppl_1) ◽  
pp. 59-59
Author(s):  
Wei Hu ◽  
Lyn Gauthier ◽  
Boris Baibakov ◽  
Jurrien Dean
Keyword(s):  
Transgenic Mice ◽  
Germ Cells ◽  
Ectopic Expression ◽  
Male Germ Cells

scholarly journals Elimination of Male Germ Cells in Transgenic Mice by the Diphtheria Toxin A Chain Gene Directed by the Histone H1t Promoter1

2000 ◽  
Vol 63 (2) ◽  
pp. 409-416 ◽  
Author(s):  
John G. Bartell ◽  
Douglas A. Fantz ◽  
Tia Davis ◽  
Michael J. Dewey ◽  
Malathi K. Kistler ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Germ Cells ◽  
Diphtheria Toxin ◽  
Chain Gene ◽  
Toxin A ◽  
Male Germ Cells ◽  
Diphtheria Toxin A ◽  
A Chain ◽  
Histone H1t

Rat transition nuclear protein 2 regulatory region directs haploid expression of reporter gene in male germ cells of transgenic mice

2001 ◽  
Vol 58 (4) ◽  
pp. 368-375 ◽  
Author(s):  
Karim Nayernia ◽  
Detlef B�hm ◽  
�zlem Topaloglu ◽  
Gregor Schl�ter ◽  
Wolfgang Engel
Keyword(s):  
Transgenic Mice ◽  
Reporter Gene ◽  
Germ Cells ◽  
Nuclear Protein ◽  
Regulatory Region ◽  
Male Germ Cells

Studies on mutations in male germ cells of transgenic mice following exposure to isopropyl methanesulfonate, ethylnitrosourea or X-ray

1994 ◽  
Vol 341 (1) ◽  
pp. 17-25 ◽  
Author(s):  
M. Katoh ◽  
T. Inomata ◽  
N. Horiya ◽  
F. Suzuki ◽  
T. Shida ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Germ Cells ◽  
Male Germ Cells ◽  
X Ray

Sequences 5′ of the homeobox of the Hox-1.4 gene direct tissue-specific expression of lacZ during mouse development

Development ◽  
1993 ◽  
Vol 117 (3) ◽  
pp. 823-833 ◽  
Author(s):  
R.R. Behringer ◽  
D.A. Crotty ◽  
V.M. Tennyson ◽  
R.L. Brinster ◽  
R.D. Palmiter ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Fusion Protein ◽  
Germ Cells ◽  
Spinal Ganglia ◽  
Control Element ◽  
Lacz Gene ◽  
Mouse Development ◽  
Specific Expression ◽  
Male Germ Cells ◽  
Cis Acting

The murine homeobox-containing gene Hox-1.4 is expressed in restricted patterns during embryogenesis and in male germ cells. To begin identification of the cis-acting elements regulating this expression, transgenic mice were generated carrying a chimeric construct that contained approx. 4 kb of 5′ flanking sequence and approx. 1 kb of structural gene, fused in frame to the E. coli lacZ gene. This construct directed expression of the resulting Hox-1.4, beta-galactosidase fusion protein in a pattern that reproduced virtually the complete embryonic and adult sites of expression of the endogenous gene. Embryonic expression of the fusion protein was first detected in mesoderm at day 8.0 of gestation (E 8.0). Between gestational ages E 8.5 to E 12.5, beta-gal expression was observed in the somites, the lateral walls of the posterior myelencephalon, the dorsal region and ventral wall of the spinal cord, spinal ganglia and prevertebrae and their surrounding mesenchyme, between presumptive ribs, as well as in mesenchymal layers in the lung, kidney and portions of the gut. Expression was also noted in the pancreas and in the supporting cells and sheath around subsets of peripheral nerves, sites that had not been detected previously. Adult expression was observed in testes, specifically in meiotic and post-meiotic male germ cells. In contrast, transgenic mice carrying 5′ deletions of the construct which leave approx. 1.2 kb or approx. 2.0 kb of Hox-1.4 sequence 5′ to the embryonic promoter, did not exhibit beta-gal staining. These deletion experiments defined at least one cis-acting control element necessary for the expression of the Hox-1.4 gene to a 2 kb region located 2 to 4 kb 5′ of the embryonic transcription start site.

scholarly journals Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry

2015 ◽  
Vol 154 ◽  
pp. 53-61 ◽  
Author(s):  
Valentina Porro ◽  
Romina Pagotto ◽  
María Belén Harreguy ◽  
Sofía Ramírez ◽  
Martina Crispo ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Transgenic Mice ◽  
Germ Cells ◽  
Environmental Estrogens ◽  
Male Germ Cells

Mutation assays in male germ cells from transgenic mice: overview of study and conclusions

1997 ◽  
Vol 388 (2-3) ◽  
pp. 111-122 ◽  
Author(s):  
J. Ashby ◽  
N.J. Gorelick ◽  
M.D. Shelby
Keyword(s):  
Transgenic Mice ◽  
Germ Cells ◽  
Male Germ Cells

scholarly journals CMV-driven expression of green fluorescent protein (GFP) in male germ cells of transgenic mice and its effect on fertility

2001 ◽  
Vol 24 (5) ◽  
pp. 300-305 ◽  
Author(s):  
Gema Villuendas ◽  
Alfonso Gutierrez-Adan ◽  
Adela Jimenez ◽  
Concepcion Rojo ◽  
Eduardo R. S. Roldan ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Germ Cells ◽  
Fluorescent Protein ◽  
Male Germ Cells ◽  
Green Fluorescent
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