scholarly journals An environmental DNA sampling method for aye-ayes from their feeding traces

2018 ◽  
Vol 8 (18) ◽  
pp. 9229-9240 ◽  
Author(s):  
Megan L. Aylward ◽  
Alexis P. Sullivan ◽  
George H. Perry ◽  
Steig E. Johnson ◽  
Edward E. Louis
Keyword(s):  
Sampling Method ◽  
Environmental Dna ◽  
Dna Sampling

scholarly journals The Foster method: Rapid and non-invasive detection of clinically relevant American Foulbrood disease levels using eDNA sampling and a dual-target qPCR assay, with its potential for other hive pathogens.

2021 ◽  
Author(s):  
John Mackay ◽  
Rebecca E Hewett ◽  
Noa T Smith ◽  
Tammy L Waters ◽  
John S Scandrett
Keyword(s):  
Sampling Method ◽  
Clinical Symptoms ◽  
Clinical Signs ◽  
Environmental Dna ◽  
American Foulbrood ◽  
Testing Methods ◽  
Non Invasive ◽  
Dna Sampling ◽  
American Foulbrood Disease ◽  
Dual Target

Clinical signs of American Foulbrood can be difficult to diagnose and thus disease is missed and leads to further spread. Diagnosis is centred on the beekeeper's skill in recognising clinical symptoms – a highly subjective and time-consuming activity. Previous testing methods have relied on sampling that necessitates dismantling the hive and/or requires multiple visits to retrieve passive samples. The Foster method is a new environmental DNA sampling method using entrance swabs together with a dual-target qPCR for AFB. The quantification data generated can be used to detect hives with clinically relevant infections, even when no visual symptoms are apparent. Such a method will be applicable to other bee pathogens and incursion pests.

Distribution and Prevalence ofMyxobolus cerebralisin Postfire Areas of Plumas National Forest: Utility of Environmental DNA Sampling

2018 ◽  
Vol 30 (2) ◽  
pp. 130-143 ◽  
Author(s):  
Christine A. Richey ◽  
Kirsten V. Kenelty ◽  
Kristina Van Stone Hopkins ◽  
Brittany N. Stevens ◽  
Beatriz Martínez-López ◽  
...  
Keyword(s):  
Environmental Dna ◽  
National Forest ◽  
Dna Sampling

scholarly journals Environmental DNA Sampling of Small‐Bodied Minnows: Performance Relative to Location, Species, and Traditional Sampling

2019 ◽  
Vol 39 (5) ◽  
pp. 1073-1085 ◽  
Author(s):  
Anthony T. Robinson ◽  
Yvette M. Paroz ◽  
Matthew J. Clement ◽  
Thomas W. Franklin ◽  
Joseph C. Dysthe ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Dna Sampling

scholarly journals A new non-invasive in situ underwater DNA sampling method for estimating genetic diversity

2020 ◽  
Vol 34 (4) ◽  
pp. 633-644
Author(s):  
Gergely Balázs ◽  
Judit Vörös ◽  
Brian Lewarne ◽  
Gábor Herczeg
Keyword(s):  
Genetic Diversity ◽  
Sampling Method ◽  
Non Invasive ◽  
Dna Sampling

scholarly journals Testing skin swabbing for DNA sampling in dendrobatid frogs

2018 ◽  
Vol 39 (2) ◽  
pp. 245-251 ◽  
Author(s):  
Eva Ringler
Keyword(s):  
Sampling Method ◽  
Microsatellite Analysis ◽  
Dry Skin ◽  
Dna Yield ◽  
Dna Quality ◽  
Amplification Success ◽  
Viable Cells ◽  
Dna Sampling ◽  
Allobates Femoralis ◽  
Polymorphic Microsatellite

Abstract Skin swabbing, a minimally invasive DNA sampling method recently proposed for adult amphibians, was tested on the dendrobatid frog Allobates femoralis. I compared DNA yield from skin swabs and toe clips by evaluating obtained DNA concentrations and purity of extracts, as well as amplification success using eleven polymorphic microsatellite loci. I also tested whether storing skin swabs for two months at −20°C affected the properties of the extract or microsatellite analysis. Results show that skin swabs of adult A. femoralis suffered from high contamination and yielded significantly lower DNA quality and quantity, resulting in insufficient genotyping success, than DNA obtained from toe clips. The relatively dry skin in dendrobatid frogs may have impeded the collection of sufficient viable cells, and the presence of skin alkaloids and microbiota in the frog mucus may lead to high contamination load of skin swabs.

Comparing Efficiency of American Fisheries Society Standard Snorkeling Techniques to Environmental DNA Sampling Techniques

2017 ◽  
Vol 37 (3) ◽  
pp. 644-651 ◽  
Author(s):  
Roy M. Ulibarri ◽  
Scott A. Bonar ◽  
Chris Rees ◽  
Jon Amberg ◽  
Bridget Ladell ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Sampling Techniques ◽  
Dna Sampling

scholarly journals Optimal survey designs for environmental DNA sampling

2017 ◽  
Vol 9 (4) ◽  
pp. 1049-1059 ◽  
Author(s):  
William H. Lugg ◽  
Josh Griffiths ◽  
Anthony R. Rooyen ◽  
Andrew R. Weeks ◽  
Reid Tingley
Keyword(s):  
Environmental Dna ◽  
Dna Sampling ◽  
Survey Designs

scholarly journals Assessing the cost‐efficiency of environmental DNA sampling

2016 ◽  
Vol 7 (11) ◽  
pp. 1291-1298 ◽  
Author(s):  
Adam S. Smart ◽  
Andrew R. Weeks ◽  
Anthony R. Rooyen ◽  
Alana Moore ◽  
Michael A. McCarthy ◽  
...  
Keyword(s):  
Cost Efficiency ◽  
Environmental Dna ◽  
Dna Sampling ◽  
The Cost

Development of tetranucleotide microsatellite loci and a non-invasive DNA sampling method for Texas horned lizards (Phrynosoma cornutum)

2011 ◽  
Vol 4 (1) ◽  
pp. 43-45 ◽  
Author(s):  
Dean A. Williams ◽  
Cory Leach ◽  
Amanda M. Hale ◽  
Kristopher B. Karsten ◽  
Emmanuela Mujica ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Sampling Method ◽  
Phrynosoma Cornutum ◽  
Non Invasive ◽  
Dna Sampling ◽  
Horned Lizards
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