scholarly journals A new non-invasive in situ underwater DNA sampling method for estimating genetic diversity

2020 ◽  
Vol 34 (4) ◽  
pp. 633-644
Author(s):  
Gergely Balázs ◽  
Judit Vörös ◽  
Brian Lewarne ◽  
Gábor Herczeg
Keyword(s):  
Genetic Diversity ◽  
Sampling Method ◽  
Non Invasive ◽  
Dna Sampling

Development of tetranucleotide microsatellite loci and a non-invasive DNA sampling method for Texas horned lizards (Phrynosoma cornutum)

2011 ◽  
Vol 4 (1) ◽  
pp. 43-45 ◽  
Author(s):  
Dean A. Williams ◽  
Cory Leach ◽  
Amanda M. Hale ◽  
Kristopher B. Karsten ◽  
Emmanuela Mujica ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Sampling Method ◽  
Phrynosoma Cornutum ◽  
Non Invasive ◽  
Dna Sampling ◽  
Horned Lizards

Validation of swabs as a non‐destructive and relatively non‐invasive DNA sampling method in fish

2010 ◽  
Vol 11 (1) ◽  
pp. 107-109 ◽  
Author(s):  
A. L. LE VIN ◽  
A. ADAM ◽  
A. TEDDER ◽  
K. E. ARNOLD ◽  
B. K. MABLE
Keyword(s):  
Sampling Method ◽  
Non Invasive ◽  
Dna Sampling ◽  
Non Destructive

scholarly journals Inventory and Characterization of Local Taro Genetic Resources in North Toraja

2018 ◽  
Vol 24 (2) ◽  
pp. 115
Author(s):  
Erina Septianti ◽  
Sahardi Sahardi
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Growth Performance ◽  
Sampling Method ◽  
Morphological Characters ◽  
Optimum Growth ◽  
High Genetic Diversity ◽  
Purposive Sampling

<p>Taro (Colocasia esculenta (L.) Schott) has been cultivated and used as an alternative food in Toraja. Local taro of North Toraja has high genetic diversity, nevertheless, they have not been well characterized. The objective of this study was to inventorying and characterizing the morphological characters of local taro from North Toraja, that could be a key component for registered and maintained as a wealth of local genetic resources. The study was conducted in North Toraja District which includes highland areas in Parodo Baruppu Village, Baruppu District and lowland areas in Mantirotiku Village, Rantepao District. Each region was selected based on initial surveys which showed that these areas were taro cultivation areas. The study was conducted from April to June 2016. Samples were selected based on purposive sampling method and were descriptively observed in-situ in the field on their optimum growth performance. Five local taro varieties were successfully inventoried from survey activities, namely Upe Ungu, Upe Kuning, Upe Putih, Monggo, and Bite. Those taros have similar characters on the shape and leave color but varied on the size and flesh fibre color of the corm. Local taro from North Toraja is widely used as snacks or vegetables as rice substitution. The advantage of North Toraja local taro is easy to cultivate and also has a savory taste, soft, good fragrant aroma, the corm skin after being boiled is very easy to peel, and the texture of the corm is smooth. The price of this local taro is quite expensive because of still difficult to find and have not been widely cultivated.</p>

scholarly journals The Foster method: Rapid and non-invasive detection of clinically relevant American Foulbrood disease levels using eDNA sampling and a dual-target qPCR assay, with its potential for other hive pathogens.

2021 ◽  
Author(s):  
John Mackay ◽  
Rebecca E Hewett ◽  
Noa T Smith ◽  
Tammy L Waters ◽  
John S Scandrett
Keyword(s):  
Sampling Method ◽  
Clinical Symptoms ◽  
Clinical Signs ◽  
Environmental Dna ◽  
American Foulbrood ◽  
Testing Methods ◽  
Non Invasive ◽  
Dna Sampling ◽  
American Foulbrood Disease ◽  
Dual Target

Clinical signs of American Foulbrood can be difficult to diagnose and thus disease is missed and leads to further spread. Diagnosis is centred on the beekeeper's skill in recognising clinical symptoms – a highly subjective and time-consuming activity. Previous testing methods have relied on sampling that necessitates dismantling the hive and/or requires multiple visits to retrieve passive samples. The Foster method is a new environmental DNA sampling method using entrance swabs together with a dual-target qPCR for AFB. The quantification data generated can be used to detect hives with clinically relevant infections, even when no visual symptoms are apparent. Such a method will be applicable to other bee pathogens and incursion pests.

Efficiency of diagnosis and treatment non-invasive and microinvasive sgamous cell cervical cancer

2020 ◽  
pp. 71-74
Author(s):  
M.M. Melnyk ◽  
◽  
S.V. Nespradko ◽  
I.V. Goncharuk ◽  
M.V. Marchenko ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Key Words ◽  
National Cancer Institute ◽  
Reproductive Function ◽  
Diagnosis And Treatment ◽  
Non Invasive ◽  
Corpus Uteri ◽  
Complex Program ◽  
Early Cervical Cancer

The objective: analyse the effectiveness of diagnosis and treatment for early cervical cancer. Materials and methods. Analysed 107 cases of women’s disease on CIN ІІІ, cancer in situ, they were on treatment in National cancer institute and Kyiv dictrict cancer dispensary from 2010 till 2015 years. Results. Diagnosed percent relapse CIN ІІІ, cancer in situ contain 4.57% uninvasive and invasive form – 0.94%. Conclusion. According diagnostic CIN ІІ and CIN ІІІ is recommended to do treatment conization and dynamic dispensary observation. Are making complex program of infection HPV16, 18. In appering of margins resection some elements of tumor after wider conization by forms of cancer in situ. Many of expansive burns in cervical glands, in making of reproductive function, going disease (nodel leiomyoma of corpus uteri etc). In perspective is accept the notion of looking after and screening research of considering infection HPV16, 18 on CIN І, CIN ІІ. Key words: cervical cancer, сancer in situ, CIN І–ІІІ, diagnostic, treatment, conization.

Genetic diversity of the extremely rare Habenaria dentata and the rare Habenaria linearifolia (Orchidaceae) in South Korea: implications for population history and conservation

2018 ◽  
Vol 151 (1) ◽  
pp. 48-60
Author(s):  
Mi Yoon Chung ◽  
Hoa Thi Quynh Le ◽  
Sungwon Son ◽  
Huai Zhen Tian ◽  
Myong Gi Chung
Keyword(s):  
Genetic Diversity ◽  
Southern China ◽  
Geographic Location ◽  
Population History ◽  
Population Variation ◽  
Conservation Strategies ◽  
Ancestral Population ◽  
Allozyme Diversity ◽  
Glacial Origin

Background and aims – Since historical events often leave an indelible mark on levels of genetic diversity of plant populations, one may indirectly infer their evolutionary history with the help of current patterns of genetic diversity. The terrestrial orchid Habenaria dentata, an element of warm-temperate/subtropical vegetation, reaches its northernmost limits in the Korean Peninsula, and thus it is extremely rare there. As H. dentata was absent from the Peninsula during the Last Glacial Maximum (LGM), it is likely to be of post-glacial origin having arrived from either a single refugium or multiple refugia. However, its rare, temperate/boreal congener H. linearifolia might have persisted in situ in either macrorefugia or microrefugia on the Peninsula during the LGM.Methods – To test which hypothesis is most appropriate for each species, we investigated levels of allozyme-based (17 loci) genetic diversity and population genetic structure in the two only known populations of H. dentata and in 12 populations of H. linearifolia.Key results – No allozyme diversity was found in H. dentata (He = 0.000), whereas H. linearifolia exhibited low within-population variation (He = 0.060) and high among-population differentiation (FST = 0.237). We found little association between populations in relation to their geographic location; several populations presented individuals belonging to different clusters.Conclusions – Our results suggest that H. dentata likely originated from a single ancestral population (perhaps from southern Japan or southern China) through post-glacial dispersal, whereas H. linearifolia probably survived the LGM in situ in microrefugia situated at low to mid-elevated regions. We further suggest that separate conservation strategies for each species should be employed, given that the two taxa have different ecological and demographic traits and harbour different levels of genetic diversity.

scholarly journals Optical nanomanipulation on solid substrates via optothermally-gated photon nudging

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Jingang Li ◽  
Yaoran Liu ◽  
Linhan Lin ◽  
Mingsong Wang ◽  
Taizhi Jiang ◽  
...  
Keyword(s):  
Colloidal Particles ◽  
Solid Substrate ◽  
Optical Scattering ◽  
Functional Nanostructures ◽  
Non Invasive ◽  
Solid Substrates ◽  
All Optical ◽  
Scattering Force ◽  
Reconfigurable Assembly

AbstractConstructing colloidal particles into functional nanostructures, materials, and devices is a promising yet challenging direction. Many optical techniques have been developed to trap, manipulate, assemble, and print colloidal particles from aqueous solutions into desired configurations on solid substrates. However, these techniques operated in liquid environments generally suffer from pattern collapses, Brownian motion, and challenges that come with reconfigurable assembly. Here, we develop an all-optical technique, termed optothermally-gated photon nudging (OPN), for the versatile manipulation and dynamic patterning of a variety of colloidal particles on a solid substrate at nanoscale accuracy. OPN takes advantage of a thin surfactant layer to optothermally modulate the particle-substrate interaction, which enables the manipulation of colloidal particles on solid substrates with optical scattering force. Along with in situ optical spectroscopy, our non-invasive and contactless nanomanipulation technique will find various applications in nanofabrication, nanophotonics, nanoelectronics, and colloidal sciences.

Non-invasive in-situ analysis of a wreath of gold leaves from the National Archaeological Museum of Taranto, Italy

Measurement ◽  
2018 ◽  
Vol 126 ◽  
pp. 164-167 ◽  
Author(s):  
Alessandro Buccolieri ◽  
Eva Degl'Innocenti ◽  
Roberto Cesareo ◽  
Alfredo Castellano ◽  
Giovanni Buccolieri
Keyword(s):  
In Situ Analysis ◽  
Non Invasive
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