Recognition of shared melanoma antigen by HLA-A2-restricted cytolytic T cell clones derived from human tumor-infiltrating lymphocytes

1993 ◽  
Vol 23 (1) ◽  
pp. 141-146 ◽  
Author(s):  
Christophe Viret ◽  
Francois Davodeau ◽  
Yannick Guilloux ◽  
Jean-Denis Bignon ◽  
Gilbert Semana ◽  
...  
Keyword(s):  
T Cell ◽  
Human Tumor ◽  
Tumor Infiltrating Lymphocytes ◽  
T Cell Clones ◽  
Melanoma Antigen ◽  
Cell Clones ◽  
Infiltrating Lymphocytes

scholarly journals Restoring the Association of the T Cell Receptor with CD8 Reverses Anergy in Human Tumor-Infiltrating Lymphocytes

Immunity ◽  
2008 ◽  
Vol 28 (3) ◽  
pp. 414-424 ◽  
Author(s):  
Nathalie Demotte ◽  
Vincent Stroobant ◽  
Pierre J. Courtoy ◽  
Patrick Van Der Smissen ◽  
Didier Colau ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Human Tumor ◽  
Tumor Infiltrating Lymphocytes ◽  
Cell Receptor ◽  
Infiltrating Lymphocytes

scholarly journals Localization of T cell clonotypes using spatial transcriptomics

2021 ◽  
Author(s):  
William H. Hudson ◽  
Lisa J. Sudmeier
Keyword(s):  
T Cell ◽  
Spatial Organization ◽  
Spatial Information ◽  
Human Tumor ◽  
Cell Receptor ◽  
Cell Repertoire ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones ◽  
Archived Samples

Spatial transcriptomics is an emerging technology that measures gene expression while preserving spatial information. Here, we present a method to determine localization of specific T cell clones by obtaining T cell receptor (TCR) sequences from spatial transcriptomics assays. Our method uses an existing commercial spatial transcriptomics platform and open-source software for analysis, allowing simple and inexpensive integration with archived samples and existing laboratory workflows. Using human brain metastasis samples, we show that TCR sequences are readily obtained from human tumor tissue and that these sequences are recapitulated by single-cell sequencing methods. This technique will permit detailed studies of the spatial organization of the human T cell repertoire, such as the identification of tumor- infiltrating and tumor-excluded T cell clones.

scholarly journals A novel strategy for rapid and efficient isolation of human tumor-specific CD4+ and CD8+ T-cell clones

2008 ◽  
Vol 331 (1-2) ◽  
pp. 13-26 ◽  
Author(s):  
Seung-Tae Lee ◽  
Shujuan Liu ◽  
Laszlo Radvanyi ◽  
Pariya Sukhumalchandra ◽  
Jeffrey J. Molldrem ◽  
...  
Keyword(s):  
T Cell ◽  
Human Tumor ◽  
Cd8 T Cell ◽  
T Cell Clones ◽  
Cell Clones ◽  
Novel Strategy
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