Author response for "Th2 cells promote eosinophil‐independent pathology in a murine model of allergic bronchopulmonary aspergillosis"

2020 ◽  
Author(s):  
Axel Dietschmann ◽  
Sebastian Schruefer ◽  
Sven Krappmann ◽  
David Voehringer
Keyword(s):  
Murine Model ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Author Response ◽  
Th2 Cells

scholarly journals Th2 cells promote eosinophil‐independent pathology in a murine model of allergic bronchopulmonary aspergillosis

2020 ◽  
Vol 50 (7) ◽  
pp. 1044-1056 ◽  
Author(s):  
Axel Dietschmann ◽  
Sebastian Schruefer ◽  
Sven Krappmann ◽  
David Voehringer
Keyword(s):  
Murine Model ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Th2 Cells

Author response for "Telomere length and oxidative stress variations in a murine model of Alzheimer’s disease progression"

2020 ◽  
Author(s):  
Katia Martínez‐González ◽  
Azul Islas‐Hernández ◽  
José Darío Martínez‐Ezquerro ◽  
Federico Bermúdez‐Rattoni ◽  
Paola Garcia‐delaTorre
Keyword(s):  
Oxidative Stress ◽  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Disease Progression ◽  
Telomere Length ◽  
Murine Model ◽  
Author Response ◽  
Model Of Alzheimer’S Disease ◽  
Stress Variations ◽  
And Oxidative Stress

scholarly journals Exposure to Traffic-Related Particulate Matter 2.5 Triggers Th2-Dominant Ocular Immune Response in a Murine Model

2020 ◽  
Vol 17 (8) ◽  
pp. 2965
Author(s):  
Hyun Soo Lee ◽  
Sehyun Han ◽  
Jeong-Won Seo ◽  
Ki-Joon Jeon
Keyword(s):  
Immune Response ◽  
Particulate Matter ◽  
Murine Model ◽  
Ocular Surface ◽  
Immunological Response ◽  
Inflammatory Cells ◽  
Th2 Cells ◽  
Fluorescein Staining ◽  
Serum Ige

Ambient particulate matter (PM), a major component of air pollution, aggravates ocular discomfort and inflammation, similarly to dry eye disease (DED) or allergies. However, the mechanism(s) by which PM induces the ocular inflammatory response is unknown. This study investigated the immunological response of traffic-related fine particulate matter (PM2.5) on the ocular surface in a murine model. C57BL/6 mice were exposed by topical application to PM2.5 or vehicle for 14 days to induce experimental environmental ocular disease. Corneal fluorescein staining and the number of ocular inflammatory cells were assessed in both groups. The expression of IL-1β, IL-6, tumor necrosis factor (TNF)-α, and mucin 5AC (MUC5AC) in the ocular surface were evaluated by real-time PCR. An immunohistochemical assay evaluated apoptosis and goblet cell density. ELISA was used to determine the levels of serum IgE and cytokines of Type 1 helper (Th1) and Type 2 helper (Th2) cells after in vitro stimulation of T cells in the draining lymph nodes (LNs). Exposure to traffic-related PM2.5 significantly increased corneal fluorescein staining and cellular toxicity in the corneal epithelium compared with the vehicle control. A significant increase in the number of CD11b+ cells on the central cornea and mast cells in the conjunctiva was observed in the PM2.5 group. Exposure to PM2.5 was associated with a significant increase in the corneal or conjunctival expression of IL-1β, IL-6, TNF, and MUC5AC compared to the vehicle, and increased maturation of dendric cells (DCs) (MHC-IIhighCD11c+) in draining LNs. In addition, PM2.5 exposure increased the level of serum IgE and Th2 cytokine production in draining LNs on day 14. In conclusion, exposure to traffic-related PM2.5 caused ocular surface damage and inflammation, which induced DC maturation and the Th2-cell-dominant allergic immune response in draining LNs.

Immunohistochemical detection of GM-CSF, IL-4 and IL-5 in a murine model of allergic bronchopulmonary aspergillosis

1996 ◽  
Vol 26 (4) ◽  
pp. 461-468 ◽  
Author(s):  
H. W. CHU ◽  
J. M. WANG ◽  
M. BOUTET ◽  
L. P. BOULET ◽  
M. LAVIOLETTE
Keyword(s):  
Murine Model ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Immunohistochemical Detection ◽  
Gm Csf
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