Mass spectrometric characterization of the zein protein composition in maize flour extracts upon protein separation by SDS‐PAGE and 2D gel electrophoresis

2019 ◽  
Vol 40 (20) ◽  
pp. 2747-2758 ◽  
Author(s):  
Paula A. Postu ◽  
Laura Ion ◽  
Gabi Drochioiu ◽  
Brindusa A. Petre ◽  
Michael O. Glocker
Keyword(s):  
Gel Electrophoresis ◽  
Protein Separation ◽  
Protein Composition ◽  
Mass Spectrometric ◽  
2D Gel Electrophoresis ◽  
Maize Flour ◽  
Sds Page ◽  
Zein Protein ◽  
2D Gel

Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: SDS-PAGE of Proteins

2006 ◽  
Vol 2006 (28) ◽  
pp. pdb.prot4559-pdb.prot4559
Author(s):  
W. R. Stochaj ◽  
T. Berkelman ◽  
N. Laird
Keyword(s):  
Gel Electrophoresis ◽  
2D Gel Electrophoresis ◽  
Ph Gradients ◽  
Sds Page ◽  
2D Gel

scholarly journals Identification of actin from hepatoma Morris 5123 cells.

1999 ◽  
Vol 46 (4) ◽  
pp. 949-959 ◽  
Author(s):  
D Nowak ◽  
A Kochman ◽  
M Malicka-Błaszkiewicz
Keyword(s):  
Gel Electrophoresis ◽  
Dnase I ◽  
2D Gel Electrophoresis ◽  
Muscle Actin ◽  
Actin Isoforms ◽  
Beta Actin ◽  
Sds Page ◽  
Actin Isoform ◽  
2D Gel ◽  
Differential Affinity

The hepatoma Morris 5123 tumor growth is accompanied by changes in actin content and polymerization (Malicka-Błaszkiewicz et al. (1995) Mat. Med. Pol., 27, 115-118; Nowak et al. (1995) J. Exp. Cancer Res. 14, 37-40). Presently actin isoforms from cytosol and cytoskeleton fractions were separated by SDS/PAGE and identified with antibodies directed against different actin isoforms. Actin isolated from the cytosol by affinity chromatography on DNase I bound to agarose shows the presence of only one protein spot on 2D gel electrophoresis corresponding to the mobility of the rabbit a skeletal muscle actin (Mr 43,000) and isoelectric point equal to 5.3. It interacts only with monoclonal anti beta actin isoform antibodies, posing the question of differential affinity of actin isoforms to DNase I.

Identification of nuclei associated proteins by 2D-gel electrophoresis and mass spectrometry

2001 ◽  
Vol 109 (1) ◽  
pp. 3-11 ◽  
Author(s):  
Jonas Bergquist ◽  
Johan Gobom ◽  
Anders Blomberg ◽  
Peter Roepstorff ◽  
Rolf Ekman
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
2D Gel Electrophoresis ◽  
Associated Proteins ◽  
2D Gel
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