Analysis of mutation in the rat Pig-a assay: II. Studies with bone marrow granulocytes

2018 ◽  
Vol 59 (8) ◽  
pp. 733-741 ◽  
Author(s):  
Azra Dad ◽  
Javier R. Revollo ◽  
Dayton M. Petibone ◽  
Mason G. Pearce ◽  
Robert H. Heflich ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Granulocytes

scholarly journals Effect of Chemical Protection and Bone Marrow Treatment on Radiation Injury in Mice

Blood ◽  
1958 ◽  
Vol 13 (7) ◽  
pp. 665-676 ◽  
Author(s):  
PAUL URSO ◽  
C. C. CONGDON ◽  
D. G. DOHERTY ◽  
RAYMOND SHAPIRA
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Radiation Injury ◽  
Combined Treatment ◽  
X Rays ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Hematopoietic Organs ◽  
X Irradiation ◽  
Bone Marrow Granulocytes

Abstract MEG (prepared from 9.0 mg. of AET) significantly modified the response of the bone marrow, peripheral blood leukocytes, spleen, thymus, body weight, hematocrit, and histology of the hematopoietic organs to lethal (900 r) and sublethal (450 r) x-irradiation in CAF1 mice. MEG reduced the effect of 900 r on the bone marrow, granulocytes of the blood, hematocrit, spleen, thymus, and body weight by a factor of approximately two. Combined treatment (MEG and isologous bone marrow) of mice exposed to 900 r of x-rays demonstrated that MEG is primarily responsible for preventing the early destruction of the bone marrow, but bone marrow injection was primarily responsible for causing a more rapid recovery of the bone marrow. In mice receiving combined treatment, recovery of the leukocytes and spleen was primarily influenced by the bone marrow injection; whereas recovery of the thymus and body weight was primarily influenced by MEG. The hematocrit values were normal after combined treatment.

Phosphorylation and cytoplasmic localization of MAPK p38 during apoptosis signaling in bone marrow granulocytes of mice irradiated in vivo and the role of amifostine in reducing these effects

2011 ◽  
Vol 113 (3) ◽  
pp. 300-307 ◽  
Author(s):  
Helena R.C. Segreto ◽  
Celina T.F. Oshima ◽  
Marcello F. Franco ◽  
Maria Regina R. Silva ◽  
Mizue I. Egami ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cytoplasmic Localization ◽  
Bone Marrow Granulocytes

scholarly journals Effect of acute alcohol intoxication on granulocyte mobilization and kinetics

Blood ◽  
1978 ◽  
Vol 52 (3) ◽  
pp. 551-559
Author(s):  
SJ Gluckman ◽  
RR MacGregor
Keyword(s):  
Bone Marrow ◽  
Heat Loss ◽  
Alcohol Intoxication ◽  
Acute Alcohol Intoxication ◽  
Endotoxin Administration ◽  
Alcohol Administration ◽  
Human Volunteers ◽  
Normal Controls ◽  
Bone Marrow Granulocytes

Granulocyte mobilization into skin abrasions in human volunteers was significantly inhibited by acute alcohol intoxication (45,-800 cells in 8 hr versus 353,000 in normal controls). Alcohol applied locally did not inhibit granulocyte delivery, and protection of the abrasion against heat loss did not reduce the inhibited delivery in intoxicated volunteers. Intoxication inhibited granulocyte adherence and local mobilization in parallel. Alcohol administration to rabbits shifted granulocytes from marginal to circulating pool in a manner similar to epinephrine. Mobilization of bone marrow granulocytes by glucocorticoid or endotoxin administration was not inhibited by intoxication, nor did it prevent the endotoxin-induced shift of granulocytes from circulating to marginal pool.

scholarly journals Polyamine synthesis in bone marrow granulocytes: effect of cell maturity and early changes following an inflammatory stimulus

Blood ◽  
1978 ◽  
Vol 51 (6) ◽  
pp. 1021-1029
Author(s):  
WH Evans ◽  
CK Grieshaber ◽  
WC Miller ◽  
SM Wilson ◽  
HA Hoffman
Keyword(s):  
Bone Marrow ◽  
Ornithine Decarboxylase ◽  
Synthetic Activity ◽  
Neutrophil Granulocytes ◽  
Decarboxylase Activity ◽  
Inflammatory Stimulus ◽  
Maximum Increase ◽  
Polyamine Synthesis ◽  
Ornithine Decarboxylase Activity ◽  
Bone Marrow Granulocytes

Enriched fractions of mature and immature neutrophil granulocytes, isolated from guinea pig bone marrow, were assayed for ornithine decarboxylase activity and polyamine content. The results show that immature granulocytes contain at least ten times more ornithine decarboxylase activity and two times more spermidine than mature granulocytes. The incorporation of 14C-ornithine into putrescine and spermidine of intact immature granulocytes was three to four times and ten times, respectively, that of mature granulocyte preparations. Six hours after an inflammatory stimulus, transient increases of 14-fold and 3-fold in the activities of ornithine decarboxylase and S-adenosyl- L-methionine decarboxylase, respectively, were observed in immature bone marrow granulocytes. At this time the incorporation of 14C- ornithine into putrescine and spermidine in bone marrow granulocytes from stimulated animals was 14 times that of cells from controls. A maximum increase in DNA synthesis in these cells during the inflammatory response occurred 6 hr after the maximum increase in the polyamine synthetic activity. Together these data suggest that polyamine synthesis in the granulocyte compartment of the bone marrow is associated chiefly with immature proliferating cells and that increased polyamine synthesis precedes increased granulocyte proliferation in the bone marrow following an inflammatory stimulus.

scholarly journals Polyamine synthesis in bone marrow granulocytes: effect of cell maturity and early changes following an inflammatory stimulus

Blood ◽  
1978 ◽  
Vol 51 (6) ◽  
pp. 1021-1029 ◽  
Author(s):  
WH Evans ◽  
CK Grieshaber ◽  
WC Miller ◽  
SM Wilson ◽  
HA Hoffman
Keyword(s):  
Bone Marrow ◽  
Ornithine Decarboxylase ◽  
Synthetic Activity ◽  
Neutrophil Granulocytes ◽  
Decarboxylase Activity ◽  
Inflammatory Stimulus ◽  
Maximum Increase ◽  
Polyamine Synthesis ◽  
Ornithine Decarboxylase Activity ◽  
Bone Marrow Granulocytes

Abstract Enriched fractions of mature and immature neutrophil granulocytes, isolated from guinea pig bone marrow, were assayed for ornithine decarboxylase activity and polyamine content. The results show that immature granulocytes contain at least ten times more ornithine decarboxylase activity and two times more spermidine than mature granulocytes. The incorporation of 14C-ornithine into putrescine and spermidine of intact immature granulocytes was three to four times and ten times, respectively, that of mature granulocyte preparations. Six hours after an inflammatory stimulus, transient increases of 14-fold and 3-fold in the activities of ornithine decarboxylase and S-adenosyl- L-methionine decarboxylase, respectively, were observed in immature bone marrow granulocytes. At this time the incorporation of 14C- ornithine into putrescine and spermidine in bone marrow granulocytes from stimulated animals was 14 times that of cells from controls. A maximum increase in DNA synthesis in these cells during the inflammatory response occurred 6 hr after the maximum increase in the polyamine synthetic activity. Together these data suggest that polyamine synthesis in the granulocyte compartment of the bone marrow is associated chiefly with immature proliferating cells and that increased polyamine synthesis precedes increased granulocyte proliferation in the bone marrow following an inflammatory stimulus.

scholarly journals The lipid A region of lipopolysaccharides from Rhizobiaceae activates bone marrow granulocytes from lipopolysaccharide-hyporesponsive C3H/HeJ andC57BL/10ScCr mice

Immunology ◽  
2000 ◽  
Vol 101 (2) ◽  
pp. 262-270 ◽  
Author(s):  
T. Pedron ◽  
R. Girard ◽  
B. Jeyaretnam ◽  
R. W. Carlson ◽  
R. Chaby
Keyword(s):  
Bone Marrow ◽  
Lipid A ◽  
Bone Marrow Granulocytes

A Dichotomy between the Release of Bone Marrow Granulocytes and Stem Cells

1977 ◽  
Vol 154 (1) ◽  
pp. 53-56 ◽  
Author(s):  
P. Quesenberry ◽  
K. Zuckerman ◽  
M. Ryan ◽  
F. Stohlman
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Granulocytes

scholarly journals Down-Modulation of L-Selectin by Lipopolysaccharide Is Not Required for Lipopolysaccharide-Induced Expression of CD14 in Mouse Bone Marrow Granulocytes

2001 ◽  
Vol 69 (7) ◽  
pp. 4287-4294 ◽  
Author(s):  
Thierry Pédron ◽  
Robert Girard ◽  
Richard Chaby
Keyword(s):  
Bone Marrow ◽  
Phorbol Ester ◽  
Polymorphonuclear Leukocytes ◽  
Cross Reactivity ◽  
Mouse Bone Marrow ◽  
Binding Studies ◽  
Induced Expression ◽  
Derivatives Of ◽  
Deficient Mice ◽  
Bone Marrow Granulocytes

ABSTRACT We established in previous studies that a constitutive lipopolysaccharide (LPS) receptor of low affinity is present on mouse bone marrow granulocytes (BMG). This yet-unidentified receptor is involved in the LPS-induced expression of a second LPS receptor, CD14. Because it has been claimed that L-selectin (CD62L) is a low-affinity LPS receptor in mature granulocytes (polymorphonuclear leukocytes), it may be asked whether this molecule could be the constitutive LPS receptor in BMG. We show in this study that l-selectin is constitutively present on BMG and is down-regulated after exposure of the cells to LPS. A phorbol ester induced a down-regulation of CD62L and blocked the LPS-induced expression of CD14. However, a metalloproteinase inhibitor (BB-3103) blocked the former but not the latter effect of PMA. We also observed an absence of cross-reactivity between LPS and a CD62L ligand (fucoidan) in binding studies with radiolabeled derivatives of the two agents. Furthermore, BMG froml-selectin-deficient mice expressed normal levels of CD14 in response to LPS. Taken together, these results demonstrate that in BMG, l-selectin is not the constitutive LPS receptor required for the LPS-induced expression of CD14.

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