Comparative investigations of the genotoxic effects of metals in the single cell gel (SCG) assay and the sister chromatid exchange (SCE) test

Genotoxic effects of pesticides are of great concern for public health due to the fact that they are widely used for both domestic and industrial purposes. Temephos is a member of organophosphorus pesticides, which is the most widely used group of chemicals against both agricultural and domestic insects. We therefore aimed in the present study to investigate the genotoxic and cytotoxic effects of temephos on human peripheral blood lymphocytes, using the cytokinesis-block micronucleus (CBMN) and sister chromatid exchange assays. The results showed that micronucleus (MN) frequency increased at concentrations of 50 and 75 ?g/ml although it was not found statically significant (p>0.05). We found that sister chromatid exchange (SCE) values at concentrations of 50 and 75 ?g/ ml were significantly higher than those obtained for the control (p<0.01). We also analyzed associations between temephos exposure and mitotic index (MI), proliferation index (PI), and cell blocked proliferation index (CBPI). There was no significant change in these values at the tested concentrations (p>0.05). It can be concluded that temephos was not cytotoxic at concentrations of 25, 50 and 75 ?g/ml. However, it may have a genotoxic potential in human peripheral lymphocytes.

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Evaluation of genotoxic effects of Apitol® (cymiazole hydrochloride) in vitro by measurement of sister chromatid exchange

Mutation Research/Genetic Toxicology and Environmental Mutagenesis ◽

10.1016/j.mrgentox.2005.10.003 ◽

2005 ◽

Vol 588 (2) ◽

pp. 152-157 ◽

Cited By ~ 20

Author(s):

Zoran Stanimirovic ◽

Jevrosima Stevanovic ◽

Slobodan Jovanovic ◽

Marko Andjelkovic

Keyword(s):

Sister Chromatid ◽

Sister Chromatid Exchange ◽

Genotoxic Effects

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Studies on genotoxic effects of superovulation in preimplantation mouse embryos using the sister chromatid exchange (SCE) assay

Cell Differentiation ◽

10.1016/0045-6039(87)90162-x ◽

1987 ◽

Vol 20 ◽

pp. 58

Keyword(s):

Sister Chromatid ◽

Sister Chromatid Exchange ◽

Mouse Embryos ◽

Genotoxic Effects ◽

Preimplantation Mouse Embryos ◽

Preimplantation Mouse

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Sister chromatid exchange in selected horse breeds (<i>Equus caballus</i>)

Archives Animal Breeding ◽

10.5194/aab-54-107-2011 ◽

2011 ◽

Vol 54 (2) ◽

pp. 107-114 ◽

Cited By ~ 1

Author(s):

E. Wójcik ◽

E. Smalec ◽

A. Danielewicz

Keyword(s):

Sister Chromatid ◽

Sister Chromatid Exchange ◽

Chromosome Instability ◽

Age Groups ◽

Peripheral Blood Lymphocytes ◽

Mean Frequency ◽

Males And Females ◽

The Mean ◽

Sce Test

Abstract. In studies of chromosome instability, the sister chromatid exchange (SCE) test is a particularly sensitive cytogenetic assay for detecting DNA damage. SCE tests of chromosome instability were performed in the group of 6 horse breeds (Pure-bred Arabian, Malapolski horse, Polish noble half-bred, Polish cold-blooded, Hucul and Polish Konik). The chromosome preparations were obtained from our in vitro culture of peripheral blood lymphocytes stained using the FPG technique. The mean number of SCEs/cell in the analysed population of horses was 5.14±1.44. The mean frequency of SCEs in the 6 analysed horse breeds varied depending on the breed. Statistically significant differences were observed between the horse breeds (P<0.01). No statistically significant differences in the number of SCEs per cell were found between the males and females (5.10±1.34 and 5.20±1.52, respectively). The horses were also assessed for the number of SCEs/cell in relation to the age of the animals. The differences between the age groups were statistically significant (P<0.01).

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