scholarly journals Sister chromatid exchange in selected horse breeds (<i>Equus caballus</i>)

2011 ◽  
Vol 54 (2) ◽  
pp. 107-114 ◽  
Author(s):  
E. Wójcik ◽  
E. Smalec ◽  
A. Danielewicz
Keyword(s):  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Chromosome Instability ◽  
Age Groups ◽  
Peripheral Blood Lymphocytes ◽  
Mean Frequency ◽  
Males And Females ◽  
The Mean ◽  
Sce Test

Abstract. In studies of chromosome instability, the sister chromatid exchange (SCE) test is a particularly sensitive cytogenetic assay for detecting DNA damage. SCE tests of chromosome instability were performed in the group of 6 horse breeds (Pure-bred Arabian, Malapolski horse, Polish noble half-bred, Polish cold-blooded, Hucul and Polish Konik). The chromosome preparations were obtained from our in vitro culture of peripheral blood lymphocytes stained using the FPG technique. The mean number of SCEs/cell in the analysed population of horses was 5.14±1.44. The mean frequency of SCEs in the 6 analysed horse breeds varied depending on the breed. Statistically significant differences were observed between the horse breeds (P<0.01). No statistically significant differences in the number of SCEs per cell were found between the males and females (5.10±1.34 and 5.20±1.52, respectively). The horses were also assessed for the number of SCEs/cell in relation to the age of the animals. The differences between the age groups were statistically significant (P<0.01).

Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera)

2015 ◽  
Vol 95 (4) ◽  
pp. 543-550 ◽  
Author(s):  
Marta Kuchta-Gładysz ◽  
Ewa Wójcik ◽  
Olga Szeleszczuk ◽  
Piotr Niedbała ◽  
Kaja Tyblewska
Keyword(s):  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Peripheral Blood Lymphocytes ◽  
Test Results ◽  
Mitotic Chromosomes ◽  
Animal Cells ◽  
Chemical Factors ◽  
The Mean ◽  
Physical And Chemical

Kuchta-Gładysz, M., Wójcik, E., Szeleszczuk, O., Niedbała, P. and Tyblewska, K. 2015. Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera). Can. J. Anim. Sci. 95: 543–550. The sister chromatid exchange (SCE) test is a cytogenetic tool with applications as a short-term screen. It is used to assess the influence of physical and chemical factors with potential mutagenic and genotoxic properties on the animal organism. The test results make it possible to eliminate mutagens, as well as helping to predict possible genetic consequences in animal cells and assess animal resistance. The mitotic chromosomes were obtained from an in vitro culture of peripheral blood lymphocytes with added bromodeoxyuridine (BrdU), at five different concentrations: 0.25, 0.5, 1.0, 2.5, and 5.0 µg mL−1. The chromosomes were stained according to the FPG method. Our analyses revealed the spontaneous SCE level in the chinchilla at the concentration of 0.5 µg mL−1. Higher concentrations of this substance have a genotoxic effect and cause damage to the DNA structure of the chromosomes by inducing additional SCEs in the chromosomes of this species. The mean SCE/cell incidence in the chinchilla population was 4.34±1.28. We investigated the effects of age on the incidence of SCE and found it significantly affected this phenomenon in both sexes.

Assessment of the genotoxic potential of tetrachlorvinphos insecticide by cytokinesis-block micronucleus and sister chromatid exchange assays

2021 ◽  
pp. 096032712110361
Author(s):  
Hayal Cobanoglu ◽  
Akin Cayir
Keyword(s):  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Chromosomal Instability ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Proliferation Index ◽  
Dna Damages ◽  
Genotoxic Potential ◽  
Index Level

Tetrachlorvinphos is an organophosphate that is classified as a carcinogen in humans by several authorities. Due to very limited data regarding the genotoxic potential, we aimed to comprehensively investigate in vitro genotoxic potential of tetrachlorvinphos. We performed our study by applying the cytokinesis-block micronucleus cytome and sister chromatid exchange (SCE) assays to human peripheral blood lymphocytes. We evaluated micronucleus (MN) and SCE frequencies and cytokinesis-block proliferation index in both exposed and non-exposed lymphocytes. We also calculated the chromosomal instability level in response to exposure by combining the results of MN and SCE. We found that MN frequency did not increase with exposure to tetrachlorvinphos (0–50 µg/ml). In contrast, we observed that SCE frequencies significantly increased with exposure to ≥5 µg/ml tetrachlorvinphos. Furthermore, exposure to tetrachlorvinphos at concentrations of 50 µg/ml induced a significant increase in chromosomal instability level ( p < 0.05). Cytokinesis-block proliferation index level did not significantly decrease in response to tetrachlorvinphos exposure. Our findings reveal that tetrachlorvinphos resulted in different DNA damages that were measured by two assays. Furthermore, our findings suggested that exposure to tetrachlorvinphos increased chromosomal instability that is a hallmark of many malignancies. We conclude that although tetrachlorvinphos does not significantly increase the MN level, the significant increase of both SCE and CIN frequencies indicates the genotoxic potential of tetrachlorvinphos in human peripheral lymphocytes. Additionally, tetrachlorvinphos is not cytotoxic in the range of tested concentrations.

scholarly journals The effect of paclitaxel alone and in combination with cycloheximide on the frequency of premature centromere division in vitro

2010 ◽  
Vol 62 (1) ◽  
pp. 63-74
Author(s):  
V. Bajic ◽  
Z. Stanimirovic ◽  
Jevrosima Stevanovic ◽  
Zorka Milicevic ◽  
Lada Zivkovic ◽  
...  
Keyword(s):  
Fluorescent In Situ Hybridization ◽  
Human Peripheral Blood ◽  
Chromosome Instability ◽  
Micronucleus Assay ◽  
Peripheral Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Premature Centromere Division ◽  
Combinational Therapies

Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono and/or combinational therapies for cancer treatment.

scholarly journals Quantitative electroencephalography as a tool for objective neurophysiological seizure diagnosis: A retrospective study

2020 ◽  
Vol 0 ◽  
pp. 1-6
Author(s):  
Rajesh Kumar ◽  
Tribhuwan Kumar ◽  
Kamlesh Jha ◽  
Yogesh Kumar
Keyword(s):  
Energy Level ◽  
Matched Control ◽  
Age Groups ◽  
Quantitative Electroencephalography ◽  
Beta Band ◽  
Significant Difference ◽  
Males And Females ◽  
The Mean ◽  
The Difference ◽  
Electrical Activities

Objectives: Seizure is the fourth most common neurological disorder in the world; it affects all age groups with equal possibility of occurrence in both males and females. Many antiepileptic drugs are available today, but its diagnosis is challenging. The present study attempted to see if seizure activities could be predicted by analyzing the pre-seizure electrical activities. The prediction may help in taking preventive measures appropriately beforehand in the individuals with seizure proneness. Material and Methods: We selected 11 generalized seizure patients and 19 control patients out of total 115 patients referred for electro-diagnostics for various reasons. EEG of the subjects recorded, segmented as per protocol, and analyzed using MATLAB and EEGLAB tools. Results: The mean energy level in alpha and beta band of the study subject was significantly lower (P = 0.04 and 0.004, respectively) as compared to the age matched control subjects. Theta and delta bands did not show any significant difference between the groups. The difference between the pre- and post-electrical seizure energy and entropy was statistically insignificant. Conclusion: The study shows that the energy level remains low in the seizure patients in the alpha and beta bands. This further goes down when electrophysiological seizure activities starts. The randomness or entropy does not alter significantly among the seizure subjects in comparison to non-seizure subjects.

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