Feed Parameters Influencing the Breeding of Mealworms (Tenebrio molitor)

With the growing public interest in edible insects, breeders are forced to increase the volume of production and shorten the life cycle of farmed insects. The exact composition of the feed is the know-how of each manufacturer. The aim of this work was to determine the general impact of individual conventional types of feed (bran, whey, raw ware potatoes) on the life cycle rate of Tenebrio molitor (TM), larval mortality and the content of fats, fatty acids and nitrogenous substances in larvae and their excrements. For the reasons of sustainability of the state of nature and waste disposal, polystyrene foam was added to the experiment as feed. The experiment was performed in two phases—rearing for 21 weeks and 5 weeks. The nitrogen content was determined using the Kjeldahl method (ISO 1871: 2009), the fat content by the Soxhlet method and the fatty acid profile using a gas chromatograph with a flame ionization detector (FID). The results of the work show that the basis of the TM feed is wheat bran, not only due to the observed low larval mortality (45% over 21 weeks) and their optimal nutritional value (nitrogenous substances 53.6% DM, fat 37.1% DM, the highest total fatty acid content) but also due to the higher speed of TM development. Lower larval mortality during rearing was found when feeding dried whey and raw ware potatoes, but the development cycle rate was low. This confirmed that the bran as a feed allowed a fast life cycle with good larval nutritional parameters. From a nutritional and reproductive point of view, TM appeared to be a suitable alternative to animal production. Polystyrene as a feed, tested for biodegradation by TM larvae, caused the highest larval mortality (75% in 21 weeks). However, the content of nitrogenous substances in the larvae increased to 69.77%, in the excrement, it even increased to 82.68%, and there was no cannibalism among the larvae. The work thus demonstrated the possibility of biodegradation of polystyrene using TM larvae with an increase in nitrogenous substances in both larvae and excrement.

Physiological and Biomechanical Responses to Cross-Country Skiing in Varying Terrain: Low- vs. High-Intensity

The purposes of our study were to investigate the physiological and biomechanical responses to low-intensity (LI) and high-intensity (HI) roller ski skating on varying terrain and compare these responses between training intensities. Nine elite male skiers performed treadmill roller skiing consisting of two 21 min sessions (7 × 3 min laps) at LI and HI with the same set inclines and intensity-dependent speeds (LI/HI: distance: 5.8/7.5 km, average speed: 16.7/21.3 km/h). Physiological and biomechanical variables were measured continuously, and each movement cycle and sub-technique employed were detected and classified with a machine learning model. Both the LI and HI sessions induced large terrain-dependent fluctuations (relative to the maximal levels) in heart rate (HR, 17.7 vs. 12.2%-points), oxygen uptake (V.O2, 33.0 vs. 31.7%-points), and muscle oxygen saturation in the triceps brachii (23.9 vs. 33.4%-points) and vastus lateralis (12.6 vs. 24.3%-points). A sub-technique dependency in relative power contribution from poles and skis exhibited a time-dependent shift from Lap 1 to Lap 7 toward gradually more ski power (6.6 vs. 7.8%-points, both p < 0.01). The terrain-dependent fluctuations did not differ between LI and HI for V.O2 (p = 0.50), whereas HR fluctuated less (p < 0.01) and displayed a time-dependent increase from Lap 2 to Lap 7 (7.8%-points, p > 0.01) during HI. Oxygen saturation shifted 2.4% points more for legs than arms from LI to HI (p > 0.05) and regarding sub-technique, 14.7% points more G3 on behalf of G2 was employed on the steepest uphill during HI (p < 0.05). Within all sub-techniques, cycle length increased two to three times more than cycle rate from LI to HI in the same terrains, while the corresponding poling time decreased more than ski contact time (all p > 0.05). In sum, both LI and HI cross-country (XC) skiing on varying terrain induce large terrain-dependent physiological and biomechanical fluctuations, similar to the patterns found during XC skiing competitions. The primary differences between training intensities were the time-dependent increase in HR, reduced relative oxygen saturation in the legs compared to the arms, and greater use of G3 on steep uphill terrain during HI training, whereas sub-technique selection, cycle rate, and pole vs. ski power distribution were similar across intensities on flat and moderately uphill terrain.

Quantitative and Qualitative Electroglottographic Wave Shape Differences in Children and Adults Using Voice Map–Based Analysis

Purpose The purpose of this study is to identify the extent to which various measurements of contacting parameters differ between children and adults during habitual range and overlap vocal frequency/intensity, using voice map–based assessment of noninvasive electroglottography (EGG). Method EGG voice maps were analyzed from 26 adults (22–45 years) and 22 children (4–8 years) during connected speech and vowel /a/ over the habitual range and the overlap vocal frequency/intensity from the voice range profile task on the vowel /a/. Mean and standard deviations of contact quotient by integration, normalized contacting speed, quotient of speed by integration, and cycle-rate sample entropy were obtained. Group differences were evaluated using the linear mixed model analysis for the habitual range connected speech and the vowel, whereas analysis of covariance was conducted for the overlap vocal frequency/intensity from the voice range profile task. Presence of a “knee” on the EGG wave shape was determined by visual inspection of the presence of convexity along the decontacting slope of the EGG pulse and the presence of the second derivative zero-crossing. Results The contact quotient by integration, normalized contacting speed, quotient of speed by integration, and cycle-rate sample entropy were significantly different in children compared to (a) adult males for habitual range and (b) adult males and adult females for the overlap vocal frequency/intensity. None of the children had a “knee” on the decontacting slope of the EGG slope. Conclusion EGG parameters of contact quotient by integration, normalized contacting speed, quotient of speed by integration, cycle-rate sample entropy, and absence of a “knee” on the decontacting slope characterize the wave shape differences between children and adults, whereas the normalized contacting speed, quotient of speed by integration, cycle-rate sample entropy, and presence of a “knee” on the downward pulse slope characterize the wave shape differences between adult males and adult females. Supplemental Material https://doi.org/10.23641/asha.15057345

Metabolomics Analysis in Different Development Stages on SP0 Generation of Rice Seeds After Spaceflight

Spaceflight is a special abiotic stress condition. In recent years, it has been confirmed that the spaceflight caused the stress response of rice seeds, and the protein level, transcription level, and methylation level will change during the planting process after returning to the ground. However, the changes at the metabolome level are not very clear. In this study, two kinds of rice seeds, Dongnong423 (DN3) and Dongnong416 (DN6), were carried on the ShiJian-10 retractable satellite (SJ-10) for 12.5 days in orbit, returned to the ground and planted in the field until the three-leaf (TLP) and tillering stage (TS). The results of antioxidant enzyme activity, soluble sugar, and electron leakage rate revealed that the spaceflight caused the stress response of rice. The TLP and TS of DN3 identified 110 and 57 different metabolites, respectively, while the TLP and TS of DN6 identified 104 and 74 different metabolites, respectively. These metabolites included amino acids, sugars, fatty acids, organic acids and secondary metabolites. We used qRT-PCR technology to explore the changes of enzyme genes in the tricarboxylic acid cycle (TCA) and amino acid metabolism pathway. Combined with the results of metabolomics, we determined that during the TLP, the TCA cycle rate of DN3 was inhibited and amino acid metabolism was activated, while the TCA cycle rate of DN6 was activated and amino acid metabolism was inhibited. In TS, the TCA cycle rate of DN3 was inhibited, and amino acid metabolism was not significantly changed, while the TCA cycle rate of DN6 was activated and amino acid metabolism was inhibited. These results suggested that the response mechanisms of the two different rice strains to spaceflight stress are different, and these differences may be reflected in energy consumption and compound biosynthesis of rice in different growth and development stages. This study provided new insights for further exploring the effects of spaceflight.

Metabolomics reveals the defense mechanism of different rice under space flight stress

BackgroundSpace flight is a special abiotic stress condition. Due to the development of space technology, its potential value has received widespread attention. Space mutation breeding is one of the important methods for human beings to solve food security. However, the molecular mechanism of space mutagenesis is still not very clear.ResultIn this study, two kinds of rice, Dongnong423 (DN3) and Dongnong416 (DN6), were carried on the SJ-10 retractable satellite for 12.5 days in orbit, returned to the ground and planted in the field until the three-leaf (TLP) and tillering stage (TS). The results of antioxidant enzyme activity, soluble sugar, and electron leakage rate revealed that the space environment caused the stress response of rice. The TLP and TS of DN3 identified 113 and 58 different metabolites, respectively. The TLP and TS of DN6 identified 107 and 77 different metabolites, respectively. These metabolites include amino acids, sugars, fatty acids, organic acids and secondary metabolites. We used qRT-PCR technology to explore the changes of enzyme genes in the TCA cycle and amino acid metabolism pathway. Combined with the results of metabolomics, we determined that during the TLP, the TCA cycle rate of DN3 was inhibited and amino acid metabolism was activated, while the TCA cycle rate of DN6 was activated and amino acid metabolism was inhibited. In TS, the TCA cycle rate of DN3 was inhibited, and amino acid metabolism was not significantly changed, while the TCA cycle rate of DN6 was activated and amino acid metabolism was inhibited. These results suggest that the space environment has different stress response mechanisms to the two rice varieties, and these differences may be reflected in energy consumption and compound biosynthesis.ConclusionsThis research uses metabolomics for the first time to explore the effects of space flight team rice. This research provides new insights for further exploring the effects of space biology and space mutation breeding.

Key Factors Related to Short Course 100 m Breaststroke Performance

Background and aim: To identify kinematic variables related to short course 100 m breaststroke performance. Methods: An automatic race analysis system was utilized to obtain start (0–15 m), turn (5 m before the wall until 10 m out), finish (95–100 m), and clean swimming (the rest of the race) segment times as well as cycle rate and cycle length during each swimming cycle from 15 male swimmers during a 100 m breaststroke race. A bivariate correlation and a partial correlation were employed to assess the relationship between each variable and swimming time. Results: Turns were the largest time contributor to the finishing time (44.30 ± 0.58%), followed by clean swimming (38.93 ± 0.50%), start (11.39 ± 0.22%), and finish (5.36 ± 0.18%). The finishing time was correlated (p < 0.001) with start segment time (r = 0.979), clean swimming time (r = 0.940), and 10 m turn-out time (r = 0.829). The clean swimming time was associated with the finishing time, but cycle rate and cycle length were not. In both start and turns, the peak velocity (i.e., take-off and push-off velocity) and the transition velocity were related to the segment time (r ≤ −0.673, p ≤ 0.006). Conclusions: Breaststroke training should focus on: (I) 15 m start with generating high take-off velocity, (II) improving clean swimming velocity by finding an optimal balance between cycle length and rate, (III) 10 m turn-out with maintaining a strong wall push-off, and (IV) establishing a high transition velocity from underwater to surface swimming.

The Effect of Maximal Speed Ability, Pacing Strategy, and Technique on the Finish Sprint of a Sprint Cross-Country Skiing Competition

Purpose: To investigate the contribution from maximal speed (Vmax) and %Vmax to the finish sprint speed obtained in a cross-country sprint in the classical and skating style, as well as the coinciding changes in kinematic patterns and the effect of pacing strategy on the %Vmax. Methods: Twelve elite male cross-country skiers performed two 80-m Vmax tests on flat terrain using the classical double-poling and skating G3 techniques, followed by 4 simulated 1.4-km sprint time trials, performed with conservative (controlled start) and positive (hard start) pacing strategies in both styles with a randomized order. In all cases, these time trials were finalized by sprinting maximally over the last 80 m (the Vmax section). Results: Approximately 85% of Vmax was obtained in the finish sprint of the 1.4-km competitions, with Vmax and %Vmax contributing similarly (R2 = 51–78%) to explain the overall variance in finish sprint speed in all 4 cases (P < .05). The changes in kinematic pattern from the Vmax to the finish sprint included 11–22% reduced cycle rate in both styles (P < .01), without any changes in cycle length. A 3.6% faster finish sprint speed, explained by higher cycle rate, was found by conservative pacing in classic style (P < .001), whereas no difference was seen in skating. Conclusions: Vmax ability and %Vmax contributed similarly to explain the finish sprint speed, both in the classic and skating styles, and independent of pacing strategy. Therefore, sprint cross-country skiers should concurrently develop both these capacities and employ technical strategies where a high cycle rate can be sustained when fatigue occurs.