Unscheduled dna synthesis and DNA repair studies of peroxyacetic and monoperoxydecanoic acids

1983 ◽  
Vol 5 (2) ◽  
pp. 177-192 ◽  
Author(s):  
W. J. Coppinger ◽  
T. K. Wong ◽  
E. D. Thompson
Keyword(s):  
Dna Repair ◽  
Dna Synthesis ◽  
Unscheduled Dna Synthesis

scholarly journals Induction of unscheduled DNA synthesis in human bone marrow cells by bifunctional alkylating agents

Blood ◽  
1979 ◽  
Vol 54 (6) ◽  
pp. 1320-1329
Author(s):  
R Lewensohn ◽  
U Ringborg
Keyword(s):  
Bone Marrow ◽  
Dna Repair ◽  
Dna Synthesis ◽  
Bone Marrow Cells ◽  
Alkylating Agents ◽  
Human Bone ◽  
Unscheduled Dna Synthesis ◽  
Repair Synthesis ◽  
Dna Repair Synthesis ◽  
Marrow Cells

A technique has been developed for handling human bone marrow cells intended for the examination of DNA repair synthesis. DNA-repair synthesis, induced by melphalan and nitrogen mustard, was measured as the incorporation of 3H-thymidine, registered by autoradiography as unscheduled DNA synthesis (UDS). Comparison of various cell populations disclosed considerable differences in their UDS level, this generally being greatest for the blast populations. During maturation of both myelopoietic and erythropoietic cells, there was a decrease in the UDS level, which was lowest for the end-cell stage. The lymphocytes and monocytes differed considerably in their capacity for UDS. The developed technique would appear to offer an opportunity for determining the capacity for DNA-repair synthesis in malignant bone marrow cells, a factor that has been linked to sensitivity to alkylating agents.

scholarly journals Induction of unscheduled DNA synthesis in human bone marrow cells by bifunctional alkylating agents

Blood ◽  
1979 ◽  
Vol 54 (6) ◽  
pp. 1320-1329 ◽  
Author(s):  
R Lewensohn ◽  
U Ringborg
Keyword(s):  
Bone Marrow ◽  
Dna Repair ◽  
Dna Synthesis ◽  
Bone Marrow Cells ◽  
Alkylating Agents ◽  
Human Bone ◽  
Unscheduled Dna Synthesis ◽  
Repair Synthesis ◽  
Dna Repair Synthesis ◽  
Marrow Cells

Abstract A technique has been developed for handling human bone marrow cells intended for the examination of DNA repair synthesis. DNA-repair synthesis, induced by melphalan and nitrogen mustard, was measured as the incorporation of 3H-thymidine, registered by autoradiography as unscheduled DNA synthesis (UDS). Comparison of various cell populations disclosed considerable differences in their UDS level, this generally being greatest for the blast populations. During maturation of both myelopoietic and erythropoietic cells, there was a decrease in the UDS level, which was lowest for the end-cell stage. The lymphocytes and monocytes differed considerably in their capacity for UDS. The developed technique would appear to offer an opportunity for determining the capacity for DNA-repair synthesis in malignant bone marrow cells, a factor that has been linked to sensitivity to alkylating agents.

Unprogrammierte DNA-Synthese (DNA-Reparatur) in Milz-und Thymuszellen der Ratte nach Einwirkung von UV-Licht, Röntgenstrahlen oder Methylmethansulfonat in vitro / Unscheduled DNA Synthesis (DNA-Repair) within Splenic and Thymic Cells of the Rat under the Influence of UV-Light, X-Irradiation and/or Methyl-methanesulfonate

1980 ◽  
Vol 35 (1-2) ◽  
pp. 106-111 ◽  
Author(s):  
K. Tempel
Keyword(s):  
Dna Repair ◽  
Dna Synthesis ◽  
Excision Repair ◽  
Uv Light ◽  
Methyl Methanesulfonate ◽  
X Rays ◽  
Unscheduled Dna Synthesis ◽  
Splenic Cells ◽  
X Irradiation

Abstract DNA -Repair, Splenocytes, Thymocytes, Irradiation, Methyl-M ethanesulfonate Unscheduled DNA synthesis (UDS) of splenic and thymic cells of the rat has been stimulated in vitro by UV-light (8-128 J × m-2), X-rays (120-3480 rd), methyl-methanesulfonate (MMS), and/or a combination of UV-light and X -irradiation. The height of U DS-induced stim ulation of incorporation of [3H] thymidine into splenic and thymic cell DNA at saturation doses of UV-light (splenic cells: 8, thymic cells: 96 J × m-2) or X -irradiation (splenic cells: 960, thymic cells:~3480 rd) suggest that the greater sensitivity of T-cells (represented by thymic cells) towards UV-light and the greater sensitivity of B-cells (represented by splenic cells) towards X-rays can be explained - at least partly - in terms of less efficient excision repair systems.

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