scholarly journals Induction of unscheduled DNA synthesis in human bone marrow cells by bifunctional alkylating agents

Blood ◽  
1979 ◽  
Vol 54 (6) ◽  
pp. 1320-1329
Author(s):  
R Lewensohn ◽  
U Ringborg
Keyword(s):  
Bone Marrow ◽  
Dna Repair ◽  
Dna Synthesis ◽  
Bone Marrow Cells ◽  
Alkylating Agents ◽  
Human Bone ◽  
Unscheduled Dna Synthesis ◽  
Repair Synthesis ◽  
Dna Repair Synthesis ◽  
Marrow Cells

A technique has been developed for handling human bone marrow cells intended for the examination of DNA repair synthesis. DNA-repair synthesis, induced by melphalan and nitrogen mustard, was measured as the incorporation of 3H-thymidine, registered by autoradiography as unscheduled DNA synthesis (UDS). Comparison of various cell populations disclosed considerable differences in their UDS level, this generally being greatest for the blast populations. During maturation of both myelopoietic and erythropoietic cells, there was a decrease in the UDS level, which was lowest for the end-cell stage. The lymphocytes and monocytes differed considerably in their capacity for UDS. The developed technique would appear to offer an opportunity for determining the capacity for DNA-repair synthesis in malignant bone marrow cells, a factor that has been linked to sensitivity to alkylating agents.

scholarly journals Induction of unscheduled DNA synthesis in human bone marrow cells by bifunctional alkylating agents

Blood ◽  
1979 ◽  
Vol 54 (6) ◽  
pp. 1320-1329 ◽  
Author(s):  
R Lewensohn ◽  
U Ringborg
Keyword(s):  
Bone Marrow ◽  
Dna Repair ◽  
Dna Synthesis ◽  
Bone Marrow Cells ◽  
Alkylating Agents ◽  
Human Bone ◽  
Unscheduled Dna Synthesis ◽  
Repair Synthesis ◽  
Dna Repair Synthesis ◽  
Marrow Cells

Abstract A technique has been developed for handling human bone marrow cells intended for the examination of DNA repair synthesis. DNA-repair synthesis, induced by melphalan and nitrogen mustard, was measured as the incorporation of 3H-thymidine, registered by autoradiography as unscheduled DNA synthesis (UDS). Comparison of various cell populations disclosed considerable differences in their UDS level, this generally being greatest for the blast populations. During maturation of both myelopoietic and erythropoietic cells, there was a decrease in the UDS level, which was lowest for the end-cell stage. The lymphocytes and monocytes differed considerably in their capacity for UDS. The developed technique would appear to offer an opportunity for determining the capacity for DNA-repair synthesis in malignant bone marrow cells, a factor that has been linked to sensitivity to alkylating agents.

scholarly journals Induction of unscheduled DNA synthesis in human myeloma cells

Blood ◽  
1980 ◽  
Vol 55 (2) ◽  
pp. 311-316
Author(s):  
R Lewensohn ◽  
U Ringborg
Keyword(s):  
Bone Marrow ◽  
Dna Synthesis ◽  
Bone Marrow Cells ◽  
Ultra Violet ◽  
Cell Types ◽  
Unscheduled Dna Synthesis ◽  
Myeloma Cells ◽  
Poorly Differentiated ◽  
The Relationship ◽  
Marrow Cells

Unscheduled DNA synthesis (UDS) induced by melphalan, nitrogen mustard and ultra-violet irradiation was studied in bone marrow cells from myeloma patients. In a previous study, normal bone marrow cells in various stages of maturation were found to display a gradual decrease in UDS parallel with the process of maturation. Myeloma cells showed a similar pattern. Poorly differentiated myeloma cells exhibited a similar level of UDS to myeloblasts and erythroblasts. Irrespective of which repair-inducing agent was used, the relationship between the levels of UDS in the various cell types was constant. This indicates that the differences in the level of UDS in the various cell types was not due to differences in the uptake of the repair-inducing agent.

scholarly journals Induction of unscheduled DNA synthesis in human myeloma cells

Blood ◽  
1980 ◽  
Vol 55 (2) ◽  
pp. 311-316 ◽  
Author(s):  
R Lewensohn ◽  
U Ringborg
Keyword(s):  
Bone Marrow ◽  
Dna Synthesis ◽  
Bone Marrow Cells ◽  
Ultra Violet ◽  
Cell Types ◽  
Unscheduled Dna Synthesis ◽  
Myeloma Cells ◽  
Poorly Differentiated ◽  
The Relationship ◽  
Marrow Cells

Abstract Unscheduled DNA synthesis (UDS) induced by melphalan, nitrogen mustard and ultra-violet irradiation was studied in bone marrow cells from myeloma patients. In a previous study, normal bone marrow cells in various stages of maturation were found to display a gradual decrease in UDS parallel with the process of maturation. Myeloma cells showed a similar pattern. Poorly differentiated myeloma cells exhibited a similar level of UDS to myeloblasts and erythroblasts. Irrespective of which repair-inducing agent was used, the relationship between the levels of UDS in the various cell types was constant. This indicates that the differences in the level of UDS in the various cell types was not due to differences in the uptake of the repair-inducing agent.

PAF and haematopoiesis: IX. Platelet-activating factor increases DNA synthesis in human bone marrow cells

1996 ◽  
Vol 15 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Yves Denizot ◽  
Fabienne Dupuis ◽  
Franck Trimoreau ◽  
Claude Verger ◽  
Annie Allegraud ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dna Synthesis ◽  
Bone Marrow Cells ◽  
Platelet Activating Factor ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Cells

scholarly journals Interleukin-3 is significantly more effective than other colony- stimulating factors in long-term maintenance of human bone marrow- derived colony-forming cells in vitro

Blood ◽  
1989 ◽  
Vol 73 (7) ◽  
pp. 1836-1841 ◽  
Author(s):  
M Kobayashi ◽  
BH Van Leeuwen ◽  
S Elsbury ◽  
ME Martinson ◽  
IG Young ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cultured Cells ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Interleukin 3 ◽  
Gm Csf ◽  
Factor G ◽  
Marrow Cells

Abstract Human bone marrow cells cultured for 21 days in the presence of recombinant human interleukin-3 (IL-3) produced up to 28 times more colony-forming cells (CFC) than could be obtained from cultures stimulated with granulocyte colony stimulating factor (G-CSF) or granulocyte-macrophage CSF (GM-CSF). IL-3-cultured cells retained a multipotent response to IL-3 in colony assays but were restricted to formation of granulocyte colonies in G-CSF and granulocyte or macrophage colonies in GM-CSF. Culture of bone marrow cells in IL-3 also led to accumulation of large numbers of eosinophils and basophils. These data contrast with the effects of G-CSF, GM-CSF, and IL-3 in seven-day cultures. Here both GM-CSF and IL-3 amplified total CFC that had similar multipotential colony-forming capability in either factor. G-CSF, on the other hand, depleted IL-3-responsive colony-forming cells dramatically, apparently by causing these cells to mature into granulocytes. The data suggest that a large proportion of IL-3- responsive cells in human bone marrow express receptors for G-CSF and can respond to this factor, the majority becoming neutrophils. Furthermore, the CFC maintained for 21 days in IL-3 may be a functionally distinct population from that produced after seven days culture of bone marrow cells in either IL-3 or GM-CSF.

Enhancement of Adenovirus-Mediated Gene Transfer to Human Bone Marrow Cells

1998 ◽  
Vol 29 (5-6) ◽  
pp. 439-451 ◽  
Author(s):  
Tsutomu Watanabe ◽  
Linda Kelsey ◽  
Ana Ageitos ◽  
Charles Kuszynski ◽  
Kazuhiko Ino ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Gene Transfer ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Cells
Sign in / Sign up

Export Citation Format

Share Document