Prospective purification and characterization of Müller glia in the mouse retina regeneration assay

Glia ◽  
2017 ◽  
Vol 65 (5) ◽  
pp. 828-847 ◽  
Author(s):  
Patrick Schäfer ◽  
Mike O. Karl
Keyword(s):  
Mouse Retina ◽  
Muller Glia ◽  
Müller Glia ◽  
Purification And Characterization ◽  
Retina Regeneration

scholarly journals The circadian clock gene Bmal1 is required to control the timing of retinal neurogenesis and lamination of Müller glia in the mouse retina

2019 ◽  
Vol 33 (8) ◽  
pp. 8745-8758 ◽  
Author(s):  
Onkar B. Sawant ◽  
Vijay K. Jidigam ◽  
Rebecca D. Fuller ◽  
Olivia F. Zucaro ◽  
Cristel Kpegba ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Clock Gene ◽  
Mouse Retina ◽  
Muller Glia ◽  
Müller Glia ◽  
Circadian Clock Gene ◽  
Retinal Neurogenesis

scholarly journals Exogenous transcription factor in Müller glia enhances damage-induced neuroregeneration in mouse retina

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S176
Author(s):  
Eun Jung Lee ◽  
Jun Woo Park ◽  
Jin Woo Kim
Keyword(s):  
Transcription Factor ◽  
Mouse Retina ◽  
Muller Glia ◽  
Müller Glia

scholarly journals STAT pathway activation limits the Ascl1-mediated chromatin remodeling required for neural regeneration from Müller glia in adult mouse retina

2019 ◽  
Author(s):  
Nikolas L. Jorstad ◽  
Matthew S. Wilken ◽  
Levi Todd ◽  
Paul Nakamura ◽  
Nick Radulovich ◽  
...  
Keyword(s):  
Adult Mouse ◽  
Neural Regeneration ◽  
Mouse Retina ◽  
Binding Motif ◽  
Muller Glia ◽  
Müller Glia ◽  
Rna Seq ◽  
Transgenic Expression ◽  
Pathway Activation ◽  
Stat Pathway

AbstractMüller glia can serve as a source for retinal regeneration in some non-mammalian vertebrates. Recently we found that this process can be induced in mouse Müller glia after injury, by combining transgenic expression of the proneural transcription factor Ascl1 and the HDAC inhibitor TSA. However, new neurons are only generated from a subset of Müller glia in this model, and identifying factors that limit Ascl1-mediated MG reprogramming could potentially make this process more efficient, and potentially useful clinically. One factor that limits neurogenesis in some non-mammalian vertebrates is the STAT pathway activation that occurs in Müller glia in response to injury. In this report, we tested whether injury induced STAT activation hampers the ability of Ascl1 to reprogram Müller glia into retinal neurons. Using a STAT inhibitor, in combination with our previously described reprogramming paradigm, we found a large increase in the ability of Müller glia to generate neurons, similar to those we described previously. Single-cell RNA-seq showed that the progenitor-like cells derived from Ascl1-expressing Müller glia have a higher level of STAT signaling than those that become neurons. Using Ascl1 ChIP-seq and DNase-seq, we found that developmentally inappropriate Ascl1 binding sites (that were unique to the overexpression context) had enrichment for the STAT binding motif. This study provides evidence that STAT pathway activation reduces the efficiency of Ascl1-mediated reprogramming in Müller glia, potentially by directing Ascl1 to inappropriate targets.

Pax6-positive müller glia cells express cell cycle markers but do not proliferate after photoreceptor injury in the mouse retina

Glia ◽  
2011 ◽  
Vol 59 (7) ◽  
pp. 1033-1046 ◽  
Author(s):  
Sandrine Joly ◽  
Vincent Pernet ◽  
Marijana Samardzija ◽  
Christian Grimm
Keyword(s):  
Cell Cycle ◽  
Mouse Retina ◽  
Muller Glia ◽  
Müller Glia ◽  
Glia Cells ◽  
Express Cell

scholarly journals HB-EGF Is Necessary and Sufficient for Müller Glia Dedifferentiation and Retina Regeneration

2012 ◽  
Vol 22 (2) ◽  
pp. 334-347 ◽  
Author(s):  
Jin Wan ◽  
Rajesh Ramachandran ◽  
Daniel Goldman
Keyword(s):  
Muller Glia ◽  
Müller Glia ◽  
Retina Regeneration ◽  
Necessary And Sufficient

scholarly journals Characterization of Müller glia and neuronal progenitors during adult zebrafish retinal regeneration

2008 ◽  
Vol 87 (5) ◽  
pp. 433-444 ◽  
Author(s):  
Ryan Thummel ◽  
Sean C. Kassen ◽  
Jennifer M. Enright ◽  
Craig M. Nelson ◽  
Jacob E. Montgomery ◽  
...  
Keyword(s):  
Muller Glia ◽  
Müller Glia ◽  
Retinal Regeneration ◽  
Adult Zebrafish ◽  
Neuronal Progenitors

bFGF and insulin lead to migration of Müller glia to photoreceptor layer in rd1 mouse retina

2021 ◽  
Vol 755 ◽  
pp. 135936
Author(s):  
Manvi Goel ◽  
Narender K. Dhingra
Keyword(s):  
Mouse Retina ◽  
Muller Glia ◽  
Müller Glia ◽  
Photoreceptor Layer ◽  
Rd1 Mouse

scholarly journals Tgfb3 collaborates with PP2A and notch signaling pathways to inhibit retina regeneration

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Mi-Sun Lee ◽  
Jin Wan ◽  
Daniel Goldman
Keyword(s):  
Notch Signaling ◽  
Signaling Pathways ◽  
Signaling Pathway ◽  
Neuronal Degeneration ◽  
Muller Glia ◽  
Müller Glia ◽  
Retina Regeneration ◽  
Retinal Injury ◽  
Multipotent Progenitors ◽  
Tgfb Signaling

Neuronal degeneration in the zebrafish retina stimulates Müller glia (MG) to proliferate and generate multipotent progenitors for retinal repair. Controlling this proliferation is critical to successful regeneration. Previous studies reported that retinal injury stimulates pSmad3 signaling in injury-responsive MG. Contrary to these findings, we report pSmad3 expression is restricted to quiescent MG and suppressed in injury-responsive MG. Our data indicates that Tgfb3 is the ligand responsible for regulating pSmad3 expression. Remarkably, although overexpression of either Tgfb1b or Tgfb3 can stimulate pSmad3 expression in the injured retina, only Tgfb3 inhibits injury-dependent MG proliferation; suggesting the involvement of a non-canonical Tgfb signaling pathway. Furthermore, inhibition of Alk5, PP2A or Notch signaling rescues MG proliferation in Tgfb3 overexpressing zebrafish. Finally, we report that this Tgfb3 signaling pathway is active in zebrafish MG, but not those in mice, which may contribute to the different regenerative capabilities of MG from fish and mammals.

scholarly journals Anatomy and spatial organization of Müller glia in mouse retina

2017 ◽  
Vol 525 (8) ◽  
pp. spc1-spc1 ◽  
Author(s):  
Jingjing Wang ◽  
Matthew L. O’Sullivan ◽  
Dibyendu Mukherjee ◽  
Vanessa M. Puñal ◽  
Sina Farsiu ◽  
...  
Keyword(s):  
Spatial Organization ◽  
Mouse Retina ◽  
Muller Glia ◽  
Müller Glia

scholarly journals Histone Deacetylase-Mediated Müller Glia Reprogramming through Her4.1-Lin28a Axis Is Essential for Retina Regeneration in Zebrafish

iScience ◽  
2018 ◽  
Vol 7 ◽  
pp. 68-84 ◽  
Author(s):  
Soumitra Mitra ◽  
Poonam Sharma ◽  
Simran Kaur ◽  
Mohammad Anwar Khursheed ◽  
Shivangi Gupta ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Muller Glia ◽  
Müller Glia ◽  
Retina Regeneration
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