Inhibition of Bile Flow in the Isolated Perfused Rat Liver by a Synthetic Parenteral Amino Acid Mixture: Associated Net Amino Acid Fluxes

Hepatology ◽  
1984 ◽  
Vol 4 (1) ◽  
pp. 69-73 ◽  
Author(s):  
Martin F. Graham ◽  
Anthony S. Tavill ◽  
Thomas C. Halpin ◽  
Loizos N. Louis
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Bile Flow ◽  
Amino Acid Mixture ◽  
Acid Mixture ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver

scholarly journals Cell swelling increases bile flow and taurocholate excretion into bile in isolated perfused rat liver

1992 ◽  
Vol 281 (3) ◽  
pp. 593-595 ◽  
Author(s):  
C Hallbrucker ◽  
F Lang ◽  
W Gerok ◽  
D Häussinger
Keyword(s):  
Bile Acid ◽  
Amino Acid ◽  
Cell Volume ◽  
Rat Liver ◽  
Bile Flow ◽  
Cell Swelling ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Volume Changes ◽  
Close Relationship

The effects of aniso-osmotically and amino-acid-induced cell-volume changes on bile flow and biliary taurocholate excretion were studied in isolated perfused rat liver. With taurocholate (100 microM) in the influent perfusate, hypo-osmotic exposure (225 mosmol/l) increased taurocholate excretion into bile and bile flow by 42 and 27% respectively, whereas inhibition by 32 and 47% respectively was observed after hyperosmotic (385 mosmol/l) exposure. The effects of aniso-moticity on taurocholate excretion into bile was observed throughout aniso-osmotic exposure, even after completion of volume-regulatory ion fluxes and were fully reversible upon re-exposure to normo-osmotic media. Hypo-osmotic cell swelling (225 mosmol/l) increased the Vmax. of taurocholate translocation from the sinusoidal compartment into bile about 2-fold. Also, cell swelling induced by glutamine and glycine stimulated both bile flow and biliary taurocholate excretion. There was a close relationship between the aniso-osmotically and amino-acid-induced change of cell volume and taurocholate excretion into bile. The data suggest that liver cell volume plays an important role in regulating bile-acid-dependent bile flow and biliary taurocholate excretion.

scholarly journals Carbohydrate formation from various precursors in neonatal rat liver

1968 ◽  
Vol 110 (4) ◽  
pp. 725-731 ◽  
Author(s):  
R. G. Vernon ◽  
Susan W. Eaton ◽  
D G Walker
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Neonatal Period ◽  
Casein Hydrolysate ◽  
Amino Acid Mixture ◽  
Neonatal Rat ◽  
Acid Mixture ◽  
Hepatic Gluconeogenesis ◽  
Threonine Dehydratase ◽  
Serine Dehydratase

1. Measurements of the net synthesis of glucose plus glycogen from various precursors in slices of glycogen-depleted livers from rats at various stages of development indicated an increase in the gluconeogenic capacity after birth with l-lactate, oxaloacetate, a casein hydrolysate, l-serine, l-threonine, l-alanine and glycerol as substrates. 2. The highest rates of incorporation of 14C-labelled precursors into glucose plus glycogen in slices of normal livers of rats of various ages were observed in such tissue preparations from neonatal animals for an amino acid mixture, l-alanine, l-serine and l-threonine. 3. The activities of rat hepatic l-serine dehydratase and l-threonine dehydratase increase rapidly after birth and show maxima about 20 days later. 4. The results provide further evidence of the increased capacity for hepatic gluconeogenesis in the neonatal period and suggest various sites of regulation of the process.

Cation Transport by Slices from the Isolated Perfused Rat Liver

1972 ◽  
Vol 50 (3) ◽  
pp. 276-278
Author(s):  
A. C. Nestruck ◽  
R. W. Furneaux
Keyword(s):  
Rat Liver ◽  
Bile Flow ◽  
Cation Transport ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Bicarbonate Buffer ◽  
Potassium Efflux ◽  
Membrane Function ◽  
Surgical Preparation

Isolated livers from fed rats were perfused for 1 h with a medium consisting of Krebs–Ringer bicarbonate buffer with added albumin and glucose. Rates of perfusate and bile flow, differences in [Formula: see text], [Formula: see text], and pH, and glucose and potassium efflux were measured. Rewarmed slices of liver taken before the surgical preparation and before the perfusion were found to be able to reverse a cation shift imposed by cold incubation. Slices of liver taken after one perfusion were not able to effect this expected cation transport. It is proposed that the perfusate used was not ideal as evidenced by the altered membrane function of slices after perfusion.

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