Rescue of the murine sarcoma virus genome from non-producer cells by the RD-114 type C virus

1972 ◽  
Vol 10 (3) ◽  
pp. 458-462 ◽  
Author(s):  
R. V. Gilden ◽  
Y. K. Lee ◽  
C. Long
1980 ◽  
Vol 35 (1) ◽  
pp. 76-92 ◽  
Author(s):  
E H Chang ◽  
J M Maryak ◽  
C M Wei ◽  
T Y Shih ◽  
R Shober ◽  
...  

Author(s):  
R. A. Al-Adhami ◽  
A. L. Chapman

Fujinaga et al reported MSV induced rat and hamster osteosarcoma which showed an occassional unusual bud in the rat induced tumors. Savage and Hackett and Hackett and Sylvester reported abnormal type C virus in UCLB cells derived from Balb/3t3 cells infected and transformed with MLV. They wer unable to demonstrate sarcoma virus activity. Fischinger and O‘Connor reported the infection of cat embryo cells by a centrifugally induced aggregate of murine sarcoma virus and feline leukemia virus designated as MSV(FelLV). This virus gave rise to a defective, focus forming virus which propagated in cat cells but not in mouse cells.In the present study the morphoiogy of the MSV(FelLV) virus obtained from Dr. Fischinger and maintained in our laboratory since 1970 will be reported. Feline embryo fibroblasts (established in our lab.) and Crandall feline kidney cells (Cutter-Haver-Lockhart, Shawnee, Kansas) were used in this study.


1975 ◽  
Vol 61 (2) ◽  
pp. 129-150 ◽  
Author(s):  
Natale Pennelli ◽  
Luigi Chieco-Bianchi ◽  
Dino Collavo ◽  
Attilio Cecchetto

Various growth phases of Moloney murine sarcoma virus (M-MSV) induced tumors in suckling and young adult BALB/c mice have been studied by light and electron microscopy. In the early phase (3-6 days following M-MSV), observations at the injection site of the thigh muscles consisted of endo- and perimysial edema, «activated» muscle satellite cells, endothelial cells and fibroblasts, scattered type C virus particles within the muscle fibers, muscle fibers and endomysial cells undergoing necrosis and macrophage and granulocyte infiltration. During the overt tumor phase (6-12 days following M-MSV), observation of neoplastic tissue disclosed proliferation of several cell types (endothelial, periosteal, fibroblasts, etc.), poorly differentiated myoblasts along with atypical rhabdo-myoblast-like cells and sarcolytes, type C virus budding from muscle fiber and myoblast plasma membrane, and intense degenerative and regenerative changes in the muscle fibers together with more profuse granulocyte infiltration. The regressive phase (13-21 days following M-MSV) presented reduced cellularity of the neoplastic tissue, a decrease in blast cells, diminishing granulocyte infiltration with contemporaneous appearance of prominent lymphocyte foci and gradual disappearance of virus particles. Although many cell types of mesenchymal origin proliferate following M-MSV infection, the above morphological findings indicate that striated muscle is a preferential site for virus replication and transformation. Furthermore, the peculiar virus cell relationships leading to cell lysis and continuous recruitment of newly infected cells have been widely documented. In the light of these findings it is suggested that, besides the host immune control of virus spread and tumor cell multiplication, the non clonal growth pattern of M-MSV induced tumors is a crucial factor in determining the spontaneous regression which occurs with high frequency in this experimental system.


1973 ◽  
Vol 138 (2) ◽  
pp. 356-363 ◽  
Author(s):  
M. Hatanaka ◽  
R. Klein ◽  
R. Toni ◽  
J. Walker ◽  
R. Gilden

A variety of cell mutants were obtained by a single 5'-bromodeoxyuridine (BrdU) treatment of an nonproducer (NP) cell line transformed by the Kirsten strain of murine sarcoma virus (Ki-MSV). Isolation procedures of these cell See PDF for Structure mutants are described. The cell mutants obtained were classified by tumorigenic potential and shedding of Type C virus particles. The cell mutants were classified into four groups: (A) tumorigenic, without particles; (B) tumorigenic, with Type C particles; (C) nontumorigenic, without particles; and (D) nontumorigenic, with Type C particles. The tumorigenic cell lines showed variability in morphology with both flat and typical transformed appearing cell lines showing equal transplantability.


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