Replication of latent Epstein-Barr virus genomes in normal and malignant lymphoid cells

The aim of this study was to report a case of primary lymphoepitheliomalike endometrial carcinoma (FIGO stage IB). A 57-year-old woman presented with an endometrial tumor showing the classic clinical and hysteroscopic aspects of endometrial carcinoma. Morphologically, the neoplasm was similar to undifferentiated nasopharyngeal carcinoma (lymphoepithelioma). Immunohistochemistry showed that the tumor cells were cyokeratins and epithelial membrane antigen positive. Leucocyte common antigen, estrogen and progesterone receptors, neuron specific enolase, cromogranin, synaptophysin, and p53 were negative. We did not find evidence of Epstein–Barr virus (EBV) infection using immunohistochemistry and polymerase chain reaction (PCR). We report the third case of an endometrial lymphoepitheliomalike carcinoma (LELC). The patient did not receive chemotherapy and is alive and free of disease 24 month after diagnosis. LELC can occur in the endometrium and in this location may not be associated with EBV infection

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Detection of Epstein-Barr virus genomes in normal human lacrimal glands.

Journal of Clinical Microbiology ◽

10.1128/jcm.28.5.1026-1032.1990 ◽

1990 ◽

Vol 28 (5) ◽

pp. 1026-1032 ◽

Cited By ~ 20

Author(s):

C A Crouse ◽

S C Pflugfelder ◽

T Cleary ◽

S M Demick ◽

S S Atherton

Keyword(s):

Epstein Barr Virus ◽

Lacrimal Glands ◽

Barr Virus ◽

Normal Human ◽

Epstein Barr ◽

Virus Genomes

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Histological and Electron Microscopic Studies of Endemic Ethmoidal Carcinomas in Cattle

Veterinary Pathology ◽

10.1177/030098587901600203 ◽

1979 ◽

Vol 16 (2) ◽

pp. 180-190 ◽

Cited By ~ 14

Author(s):

A. Pospischil ◽

T. Haenichen ◽

H. Schaeffler

Keyword(s):

Epstein Barr Virus ◽

Undifferentiated Carcinoma ◽

Olfactory Mucosa ◽

Lymphoid Cells ◽

Electron Microscopic ◽

Barr Virus ◽

Human Nasopharyngeal Carcinoma ◽

Microscopic Studies ◽

Epstein Barr ◽

Tumor Types

In five cases of endemic ethmoidal carcinoma in cattle from the Dominican Republic three tumor types could be classified: undifferentiated carcinoma (3), adenocarcinoma (1), and squamous cell carcinoma (1). Electron microscopy showed that the tumor cells in undifferentiated carcinomas closely resembled the cells of the normal olfactory mucosa. This was especially true for the dark cells of Bowman's gland. Ultrastructurally, the lymphoid cells of the undifferentiated bovine carcinoma resembled the lymphoid cells of human nasopharyngeal carcinoma being closely associated with Epstein-Barr Virus. This and epidemiological observations suggested a viral cause of endemic ethmoidal carcinoma.

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Transcription of latent Epstein-Barr virus genomes in human epithelial/Burkitt hybrid cells

Virology ◽

10.1016/0042-6822(77)90032-0 ◽

1977 ◽

Vol 82 (1) ◽

pp. 63-68 ◽

Cited By ~ 18

Author(s):

Akiko Tanaka ◽

Meihan Nonoyama ◽

Ronald Glaser

Keyword(s):

Epstein Barr Virus ◽

Hybrid Cells ◽

Barr Virus ◽

Epstein Barr ◽

Virus Genomes

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The potential usefulness of sputum cytology in the conclusive diagnosis of methotrexate-associated lymphoproliferative disorders: A case report

SAGE Open Medical Case Reports ◽

10.1177/2050313x19836017 ◽

2019 ◽

Vol 7 ◽

pp. 2050313X1983601 ◽

Cited By ~ 1

Author(s):

Seiya Mizuguchi ◽

Kenichi Mizutani ◽

Manabu Yamashita ◽

Hiroshi Minato ◽

Sohsuke Yamada

Keyword(s):

B Cell ◽

Lymphoproliferative Disorder ◽

Epstein Barr Virus ◽

B Cell Lymphoma ◽

Lymphoid Cells ◽

Sputum Cytology ◽

Barr Virus ◽

Large B Cell Lymphoma ◽

Epstein Barr ◽

Conclusive Diagnosis

Background: Methotrexate has been used as an anchor drug for the treatment of rheumatoid arthritis and is considered to be a cause of methotrexate-associated lymphoproliferative disorder. Spontaneous regression can occur after withdrawal of methotrexate and may be associated with Epstein–Barr virus positivity and non-diffuse large B cell lymphoma histological type. Methotrexate-associated lymphoproliferative disorders are often diagnosed pathologically by lung biopsy. To the best of our knowledge, there have been no studies on the cytological diagnosis of methotrexate-associated lymphoproliferative disorder using sputum smears. Case: A 70-year-old man, who was diagnosed with rheumatoid arthritis 13 years previously and who had been treated with methotrexate, presented shortness of breath and productive cough. Methotrexate-associated lymphoproliferative disorder was suspected as the sputum cytology showed many atypical lymphoid cells with hyperchromatic enlarged nuclei, foamy cytoplasm and distinct nucleoli. Chest computed tomography revealed multiple nodular shadows with interstitial pneumonia in the bilateral lower lung field. A lung biopsy specimen contained atypical lymphoid cells that were immunohistochemically positive for CD20 and MUM-1, and weakly positive for bcl-6, but negative for CD3 and CD10. There were no Epstein–Barr virus-infectious lymphoid cells by ISH-EBER. He was finally diagnosed with methotrexate-associated lymphoproliferative disorder (non-germinal center B-cell-like diffuse large B cell lymphoma histological type). Most of the nodules disappeared spontaneously following the withdrawal of methotrexate. Discussion and conclusion: A cytologically conclusive diagnosis of methotrexate-associated lymphoproliferative disorder may be reached using sputum smears and clinical information.

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