scholarly journals Determination of the metabolic index using the fluorescence lifetime of free and bound nicotinamide adenine dinucleotide using the phasor approach

2019 ◽  
Vol 12 (11) ◽  
Author(s):  
Suman Ranjit ◽  
Leonel Malacrida ◽  
Milka Stakic ◽  
Enrico Gratton
Keyword(s):  
Fluorescence Lifetime ◽  
Nicotinamide Adenine Dinucleotide ◽  
Nicotinamide Adenine ◽  
Metabolic Index

Using multi-walled carbon nanotubes to enhance coimmobilization of poly(azure A) and poly(neutral red) for determination of nicotinamide adenine dinucleotide and hydrogen peroxide

RSC Advances ◽  
2014 ◽  
Vol 4 (85) ◽  
pp. 45566-45574 ◽  
Author(s):  
Kuo Chiang Lin ◽  
A. T. Ezhil Vilian ◽  
Shen Ming Chen
Keyword(s):  
Carbon Nanotubes ◽  
Hydrogen Peroxide ◽  
Nicotinamide Adenine Dinucleotide ◽  
Neutral Red ◽  
Nicotinamide Adenine ◽  
Hybrid Films ◽  
Azure A ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Illustration of electro-codeposition of azure A and neutral red hybrid films using high ly conductive and steric MWCNTs as a template.

scholarly journals DETERMINATION OF GLUTATHIONE REDUCTASE ACTIVITY IN MICROGRAM SAMPLES OF TISSUE, QUANTITATIVE HISTOLOGIC DISTRIBUTION OF THE ENZYME IN THE RAT ADRENAL AND EFFECT OF ADRENOCORTICOTROPIN

1968 ◽  
Vol 16 (3) ◽  
pp. 185-190 ◽  
Author(s):  
NORBERTO A. SCHOR ◽  
DAVID GLICK
Keyword(s):  
Glutathione Reductase ◽  
Nicotinamide Adenine Dinucleotide ◽  
Reductase Activity ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Border Region ◽  
Nicotinamide Adenine ◽  
Subcutaneous Injections ◽  
Glutathione Reductase Activity ◽  
Reduced Nicotinamide Adenine Dinucleotide

A fluorometric method for determination of glutathione reductase activity in microgram samples of tissue, i.e., microtome sections, based on measurement of the decrease of reduced nicotinamide adenine dinucleotide phosphate due to its oxidation on reaction with oxidized glutathione, was developed and applied to the quantitative histologic distribution of the enzyme in the adrenal gland of the rat. Single subcutaneous injections of adrenocorticotropin in saline solution (25 mg/kg) produced little change of enzyme activity in any of the histologic zones, although there was some tendency for the peak activity to shift from fasciculata to the fascicular-reticular border region. The possible interrelationship of glutathione reductase with ascorbic acid and reduced nicotinamide adenine dinucleotide phosphate in adrenal function was considered.

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