Tissue clearing‐based method for unobstructed three‐dimensional imaging of mouse penis with subcellular resolution

2020 ◽  
Vol 13 (7) ◽  
Author(s):  
Paweł Matryba ◽  
Artur Wolny ◽  
Monika Pawłowska ◽  
Anna Sosnowska ◽  
Zuzanna Rydzyńska ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Subcellular Resolution

scholarly journals Three-dimensional imaging of KNDy neurons in the mammalian brain using optical tissue clearing and multiple-label immunocytochemistry

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Aleisha M. Moore ◽  
Kathryn A. Lucas ◽  
Robert L. Goodman ◽  
Lique M. Coolen ◽  
Michael N. Lehman
Keyword(s):  
Three Dimensional ◽  
Mammalian Brain ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Kndy Neurons

scholarly journals Three-dimensional imaging of intact porcine cochlea using tissue clearing and custom-built light-sheet microscopy

2020 ◽  
Vol 11 (11) ◽  
pp. 6181
Author(s):  
Adele Moatti ◽  
Yuheng Cai ◽  
Chen Li ◽  
Tyler Sattler ◽  
Laura Edwards ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Light Sheet ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Light Sheet Microscopy

Three-Dimensional Imaging of Intraplaque Neovascularization in a Mouse Model of Advanced Atherosclerosis

2020 ◽  
Vol 57 (6) ◽  
pp. 348-354 ◽  
Author(s):  
Paola Perrotta ◽  
Isabel Pintelon ◽  
Margreet R. de Vries ◽  
Paul H.A. Quax ◽  
Jean-Pierre Timmermans ◽  
...  
Keyword(s):  
Mouse Model ◽  
Three Dimensional ◽  
Heterozygous Mutation ◽  
Plaque Morphology ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Dibenzyl Ether ◽  
Fibrillin 1 ◽  
Secondary Antibodies ◽  
Reliable Methods

Multiple lines of evidence suggest that intraplaque (IP) neovascularization promotes atherosclerotic plaque growth, destabilization, and rupture. However, pharmacological inhibition of IP neovascularization remains largely unexplored due to the limited number of animal models that develop IP neovessels and the lack of reliable methods for visualizing IP angiogenesis. Here, we applied 3D confocal microscopy with an optimized tissue-clearing process, immunolabeling-enabled three-dimensional imaging of solvent-cleared organs, to visualize IP neovessels in apolipoprotein E-deficient (ApoE<sup>−/−</sup>) mice carrying a heterozygous mutation (C1039+/−) in the fibrillin-1 gene. Unlike regular ApoE<sup>−/−</sup> mice, this mouse model is characterized by the presence of advanced plaques with evident IP neovascularization. Plaques were stained with antibodies against endothelial marker CD31 for 3 days, followed by incubation with fluorescently labeled secondary antibodies. Subsequent tissue clearing with dichloromethane (DCM)/methanol, DCM, and dibenzyl ether allowed easy visualization and 3D reconstruction of the IP vascular network while plaque morphology remained intact.

scholarly journals Continuous subcellular resolution three-dimensional imaging on intact macaque brain

2021 ◽  
Author(s):  
Can Zhou ◽  
Xiaoquan Yang ◽  
Shihao Wu ◽  
Qiuyuan Zhong ◽  
Ting Luo ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Subcellular Resolution

scholarly journals Optical Tissue Clearing Enables Rapid, Precise and Comprehensive Assessment of Three-Dimensional Morphology in Experimental Nerve Regeneration Research

2021 ◽  
Author(s):  
Simeon C. Daeschler ◽  
Jennifer Zhang ◽  
Tessa Gordon ◽  
Gregory H. Borschel
Keyword(s):  
Nerve Regeneration ◽  
Nerve Repair ◽  
Three Dimensional ◽  
Retrograde Labelling ◽  
Tissue Clearing ◽  
Processing Times ◽  
Morphological Studies ◽  
Target Organs ◽  
Subcellular Resolution ◽  
Rapid Processing

AbstractMorphological analyses are key outcome assessments for nerve regeneration studies but are historically limited to tissue sections. Novel optical tissue clearing techniques enabling three-dimensional imaging of entire organs at a subcellular resolution have revolutionized morphological studies of the brain. To extend their applicability to experimental nerve repair studies we adapted these techniques to nerves and their motor and sensory targets in rats. The solvent-based protocols rendered harvested peripheral nerves and their target organs transparent within 24 h while preserving tissue architecture and fluorescence. Optical clearing is compatible with conventional laboratory techniques, including retrograde labelling studies, and computational image segmentation, providing fast and precise cell quantitation. Further, optically cleared organs enable three-dimensional morphometry at an unprecedented scale including dermatome-wide innervation studies, tracing of intramuscular nerve branches and mapping of neurovascular networks. Given their wide-ranging applicability, rapid processing times and low costs, tissue clearing techniques are likely to be a key technology for next-generation nerve repair studies.

scholarly journals Investigation of Postnatal Craniofacial Bone Development with Tissue Clearing-Based Three-Dimensional Imaging

2019 ◽  
Vol 28 (19) ◽  
pp. 1310-1321 ◽  
Author(s):  
Wenjing Luo ◽  
Yating Yi ◽  
Dian Jing ◽  
Shiwen Zhang ◽  
Yi Men ◽  
...  
Keyword(s):  
Bone Development ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Craniofacial Bone
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