Bone formation using novel interconnected porous calcium hydroxyapatite ceramic hybridized with cultured marrow stromal stem cells derived from Green rat

2004 ◽  
Vol 69A (3) ◽  
pp. 454-461 ◽  
Author(s):  
Yoshiyuki Ito ◽  
Nobuhiro Tanaka ◽  
Yoshinori Fujimoto ◽  
Yuji Yasunaga ◽  
Osamu Ishida ◽  
...  
2015 ◽  
Vol 27 (4) ◽  
pp. 1493-1505 ◽  
Author(s):  
H. Kang ◽  
H. Chen ◽  
P. Huang ◽  
J. Qi ◽  
N. Qian ◽  
...  

2015 ◽  
Vol 15 (2) ◽  
pp. 281-289 ◽  
Author(s):  
Li Chen ◽  
Kaikai Shi ◽  
Charles Edward Frary ◽  
Nicholas Ditzel ◽  
Huimin Hu ◽  
...  

Stem Cells ◽  
2014 ◽  
Vol 32 (4) ◽  
pp. 902-912 ◽  
Author(s):  
Li Chen ◽  
Kim HolmstrØm ◽  
Weimin Qiu ◽  
Nicholas Ditzel ◽  
Kaikai Shi ◽  
...  

2002 ◽  
Vol 20 (6) ◽  
pp. 587-591 ◽  
Author(s):  
Songtao Shi ◽  
Stan Gronthos ◽  
Shaoqiong Chen ◽  
Anand Reddi ◽  
Christopher M. Counter ◽  
...  

Author(s):  
Basem M. Abdallah ◽  
Hany M. Khattab

: The isolation and culture of murine bone marrow-derived mesenchymal stromal stem cells (mBMSCs) have attracted great interest in terms of the pre-clinical applications of stem cells in tissue engineering and regenerative medicine. In addition, culturing mBMSCs is important for studying the molecular mechanisms of bone remodelling using relevant transgenic mice. Several factors have created challenges in the isolation and high-yield expansion of homogenous mBMSCs; these factors include low frequencies of bone marrow-derived mesenchymal stromal stem cells (BMSCs) in bone marrow, variation among inbred mouse strains, contamination with haematopoietic progenitor cells (HPCs), the replicative senescence phenotype and cellular heterogeneity. In this review, we provide an overview of nearly all protocols used for isolating and culturing mBMSCs with the aim of clarifying the most important guidelines for culturing highly purified mBMSC populations retaining in vitro and in vivo differentiation potential.


2014 ◽  
Vol 9 (4) ◽  
pp. 306-318 ◽  
Author(s):  
Loic Reppel ◽  
Talar Margossian ◽  
Layale Yaghi ◽  
Philippe Moreau ◽  
Nathalie Mercier ◽  
...  

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