De Novo Bone Formation by Adult Adipose-Derived Stem Cells/Alginate/Collagen Matrix in Prefabricated Vascularized Capsules in Rats: Application to Free Tissue Transfer

2006 ◽  
Vol 22 (08) ◽  
Author(s):  
Minh-Doan Nguyen ◽  
Hans Suchy ◽  
Hedge Jagadish ◽  
Jamie Schall ◽  
Christopher Chambers ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
De Novo ◽  
Free Tissue Transfer ◽  
Collagen Matrix ◽  
Adipose Derived Stem Cells ◽  
Tissue Transfer

scholarly journals Adipose-derived stem cells transfected with pEGFP-OSX enhance bone formation during distraction osteogenesis

2014 ◽  
Vol 15 (5) ◽  
pp. 482-490 ◽  
Author(s):  
Qing-guo Lai ◽  
Shao-long Sun ◽  
Xiao-hong Zhou ◽  
Chen-ping Zhang ◽  
Kui-feng Yuan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Distraction Osteogenesis ◽  
Adipose Derived Stem Cells ◽  
Enhance Bone Formation

scholarly journals The Role of Scaffold Architecture and Composition on the Bone Formation by Adipose-Derived Stem Cells

2014 ◽  
Vol 20 (1-2) ◽  
pp. 434-444 ◽  
Author(s):  
Heidi A. Declercq ◽  
Tim Desmet ◽  
Peter Dubruel ◽  
Maria J. Cornelissen
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Adipose Derived Stem Cells ◽  
Scaffold Architecture

scholarly journals Canonical Wnts function as potent regulators of osteogenesis by human mesenchymal stem cells

2009 ◽  
Vol 185 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Guizhong Liu ◽  
Sapna Vijayakumar ◽  
Luca Grumolato ◽  
Randy Arroyave ◽  
HuiFang Qiao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Wnt Signaling ◽  
De Novo ◽  
Human Mesenchymal Stem Cells ◽  
Mitogen Activated Protein Kinase ◽  
Bone Homeostasis

Genetic evidence indicates that Wnt signaling is critically involved in bone homeostasis. In this study, we investigated the functions of canonical Wnts on differentiation of adult multipotent human mesenchymal stem cells (hMSCs) in vitro and in vivo. We observe differential sensitivities of hMSCs to Wnt inhibition of osteogenesis versus adipogenesis, which favors osteoblastic commitment under binary in vitro differentiation conditions. Wnt inhibition of osteogenesis is associated with decreased expression of osteoblastic transcription factors and inhibition of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase activation, which are involved in osteogenic differentiation. An hMSC subpopulation exhibits high endogenous Wnt signaling, the inhibition of which enhances osteogenic and adipogenic differentiation in vitro. In an in vivo bone formation model, high levels of Wnt signaling inhibit de novo bone formation by hMSCs. However, hMSCs with exogenous expression of Wnt1 but not stabilized β-catenin markedly stimulate bone formation by naive hMSCs, arguing for an important role of a canonical Wnt gradient in hMSC osteogenesis in vivo.

scholarly journals Addition of Adipose-Derived Stem Cells to Mesenchymal Stem Cell Sheets Improves Bone Formation at an Ectopic Site

2016 ◽  
Vol 17 (2) ◽  
pp. 70 ◽  
Author(s):  
Zhifa Wang ◽  
Zhijin Li ◽  
Taiqiang Dai ◽  
Chunlin Zong ◽  
Yanpu Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Formation ◽  
Adipose Derived Stem Cells ◽  
Cell Sheets

208 The effect of copper on the differentiation of adipose-derived stem cells into osteoblasts

2019 ◽  
Vol 31 (1) ◽  
pp. 229
Author(s):  
T. Bane ◽  
L. Siegel ◽  
J. Bertels ◽  
K. Ratz ◽  
M. Rubessa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Lysyl Oxidase ◽  
Collagen Matrix ◽  
Negative Control ◽  
Nodule Formation ◽  
Adipose Derived Stem Cells ◽  
Osteogenic Medium ◽  
Alizarin Red ◽  
Significant Difference ◽  
Positive Effect

Large bone defects present a tremendous challenge to the treating surgeon. Tissue engineering using scaffolds of various sizes and shapes that contain stem cells and other osteoinductive molecules offer a potential solution to this difficult problem. The aim of this project was to evaluate whether osteogenic medium infused with copper influences the differentiation of adipose-derived stem cells (ASC) into osteoblasts. Copper is a key cofactor for lysyl oxidase, an enzyme involved in producing a collagen matrix through which bone can grow. Lysyl oxidase expression is up-regulated in bone marrow stromal cells (Khosravi et al. 2014 PLoS One 9, e100669). Our hypothesis was that the presence of copper in the osteogenic medium would positively influence the number of osteoblastic nodules formed. Swine ASC were isolated as described (Monaco et al. 2009 Open Tissue Eng. Regen. Med. J. 2, 20-33). The ASC were divided in 7 different treatments: 5 concentrations of copper in the osteogenic medium (0.1, 1, 10, 50, and 100 µM) plus 2 control treatments (osteogenic medium without copper and a negative control, DMEM). The medium was changed twice a week for 4 weeks. The experiment was replicated 6 times. At the end of the culture period, cells were stained with Alizarin Red S and Von Kossa stains. In each well, we counted the total number of nodules that were either formed or forming. Data were analysed using the generalized linear model (GLM) procedure (SPSS Inc./IBM Corp., Armonk, NY). The least significant difference (l.s.d.) post hoc test was used to perform statistical multiple comparison. The α-level was set at 0.05. The results showed that more nodules were formed in the 0.1 and 1 µM copper groups compared with the osteogenic control, but there was no statistical difference between those 2 treatments. Table 1 illustrates the total number of formed and forming nodules in addition to their standard deviation. There was a positive effect on nodule formation when copper concentrations of 0.1 and 1 µM were added to the osteogenic medium. In contrast, copper concentrations of 50 and 100 µM had a cytotoxic effect. These results confirm that low concentrations of copper have a positive effect on osteogenesis. This preliminary experiment is the first step towards the analysis of the behaviour of ASC on scaffolds with copper incorporated into their matrix. Table 1.The average number (standard deviations in parentheses) of total formed and forming osteoblast nodules compared between treatment groups

scholarly journals Delivery of Phenamil Enhances BMP-2-Induced Osteogenic Differentiation of Adipose-Derived Stem Cells and Bone Formation in Calvarial Defects

2015 ◽  
Vol 21 (13-14) ◽  
pp. 2053-2065 ◽  
Author(s):  
Jiabing Fan ◽  
Choong Sung Im ◽  
Zhong-Kai Cui ◽  
Mian Guo ◽  
Olga Bezouglaia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Calvarial Defects

Mussel Adhesion-Inspired Reverse Transfection Platform Enhances Osteogenic Differentiation and Bone Formation of Human Adipose-Derived Stem Cells

Small ◽  
2016 ◽  
Vol 12 (45) ◽  
pp. 6266-6278 ◽  
Author(s):  
Jisoo Shin ◽  
Jung Ho Cho ◽  
Yoonhee Jin ◽  
Kisuk Yang ◽  
Jong Seung Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Reverse Transfection

scholarly journals The synergistic effect of NELL1 and adipose-derived stem cells on promoting bone formation in osteogenesis imperfecta treatment

2020 ◽  
Vol 128 ◽  
pp. 110235
Author(s):  
Yi Liu ◽  
Mingyan Ju ◽  
Zihan Wang ◽  
Jiaci Li ◽  
Chenyi Shao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Synergistic Effect ◽  
Osteogenesis Imperfecta ◽  
Adipose Derived Stem Cells

scholarly journals Mineral deposition and vascular invasion of hydroxyapatite reinforced collagen scaffolds seeded with human adipose-derived stem cells

2019 ◽  
Vol 23 (1) ◽  
Author(s):  
Holly E. Weiss-Bilka ◽  
Matthew J. Meagher ◽  
Joshua A. Gargac ◽  
Glen L. Niebur ◽  
Ryan K. Roeder ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Vascular Invasion ◽  
High Porosity ◽  
Adipose Derived Stem Cells ◽  
Micro Computed Tomography ◽  
Collagen Scaffolds ◽  
Tissue Scaffold ◽  
Mineral Deposition ◽  
Pre Treatment

Abstract Background Collagen-based scaffolds reinforced with hydroxyapatite (HA) are an attractive choice for bone tissue engineering because their composition mimics that of bone. We previously reported the development of compression-molded collagen-HA scaffolds that exhibited high porosity, interconnected pores, and mechanical properties that were well-suited for surgical handling and fixation. The objective of this study was to investigate these novel collagen-HA scaffolds in combination with human adipose-derived stem cells (hASCs) as a template for bone formation in a subcutaneous athymic mouse model. Methods Collagen-HA scaffolds and collagen-only scaffolds were fabricated as previously described, and a clinically approved bone void filler was used as a control for the material. Constructs were seeded with hASCs and were pre-treated with either control or osteogenic media. A cell-free group was also included. Scaffolds were implanted subcutaneously in the backs of athymic nude mice for 8 weeks. Mineral deposition was quantified via micro-computed tomography. Histological and immunofluorescence images of the explants were used to analyze their vascular invasion, remodeling and cellularity. Results Cell-free collagen-HA scaffolds and those that were pre-seeded with osteogenically differentiated hASCs supported mineral deposition and vascular invasion at comparable rates, while cell-seeded constructs treated with the control medium showed lower mineralization after implantation. HA-reinforcement allowed collagen constructs to maintain their shape, provided improved cell-tissue-scaffold integration, and resulted in a more organized tissue when pre-treated in an osteogenic medium. Scaffold type and pre-treatment also determined osteoclast activity and therefore potential remodeling of the constructs. Conclusions The results of this study cumulatively indicate that treatment medium and scaffold composition direct mineralization and angiogenic tissue formation in an ectopic model. The data suggest that it may be necessary to match the scaffold with a particular cell type and cell-specific pre-treatment to achieve optimal bone formation.

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