wharton's jelly
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2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Hager Abouelnaga ◽  
Doaa El-Khateeb ◽  
Yasmine Moemen ◽  
Ashraf El-Fert ◽  
Mohamed Elgazzar ◽  
...  

Abstract Background Isolation of post-partum umbilical cord Wharton’s jelly stem cells has gained attention as an alternative source of the bone marrow. Because easy isolation, lack of ethical concerns, and the presence of both embryonic and adult stem cells have made them a valuable source for use in therapeutic applications and regenerative medicine. The study utilized a modified protocol using in-house human pooled cord blood serum for isolation and expansion of the mesenchymal stem cells obtained from the human umbilical cord Wharton’s jelly. Cell proliferation and population doubling time and tri-lineage differentiation were assessed, and the expressions of mesenchymal cell surface markers CD44, CD90, CD105, and CD34 were assessed by flow cytometry and RT-PCR. The genetic stability of the isolated cells was assessed by chromosomal karyotype. Results The isolated cells displayed fibroblastic-like morphology and tri-lineage differentiation into adipocyte, chondrocyte, and osteocyte. The isolated cells maintained the proliferative competence with a doubling time ranged from 38 to 42h and corresponded well with the standard positive and negative molecular markers (CD44+, CD90+, CD 105+, and CD34−). Cell senescence occurred at the later passage of the cells (P15) affecting, about 25% of the population. Metaphases spread of the cells showed normal diploid karyotypes, with typical chromosomal plates indicating genetic stability of the isolated cells. Conclusion The primary cultures exhibited success in isolating the umbilical cord Wharton’s jelly mesenchymal stem cells, which maintained their tri-lineage differentiation potential, phenotypes and karyotype characteristics on further passage and expansion.


2021 ◽  
Vol 10 (2) ◽  
pp. 676-682
Author(s):  
Mustofa Mustofa ◽  
Evy Kurniawaty ◽  
Arif Yudho Prabowo ◽  
Novita Carolia

Pendahuluan; Luka post hecting adalah luka yang terjadi akibat tindakan medis, secara fisiologis tubuh akan mengalami proses penyembuhan luka. D gel merupakan gel yang mengandung siloxane cyclic dan vitamin C yang dapat digunakan untuk penyembuhan luka post hecting, tetapi salah satu pengobatan luka lain yang saat ini dapat digunakan adalah ekstrak sel punca mesenkimal tali pusat manusia (WJMSCs). Tujuan; mengetahui waktu perbedaan penyembuhan luka post hecting antara ekstrak WJMSCs dengan D gel. Metode; menggunakan penelitian eksperimental laboratorik yang menggunakan 21 ekor tikus putih jantan (Rattus novergicus) galur Sprague dawley yang dikelompokkan menjadi tiga kelompok perlakuan berbeda. Perlakuan dibagi atas kelompok K: kontrol negatif (povidone iodine), P1: ekstrak WJMSCs, dan P2: D gel. Pengamatan terhadap luka post hecting dilakukan selama 14 hari menggunakan kriteria Nagaoka dan data dianalisis menggunakan uji statistik deskriptif kategorik serta Kruskal-Wallis. Hasil; menunjukkan adanya perbedaan waktu penyembuhan luka post hecting antara ekstrak WJMSC dengan D gel secara bermakna dengan p value= 0,03, dengan waktu penyembuhan luka kelompok K: 12,7 hari, kelompok P1: 7 hari, dan kelompok P2: 11 hari. Kesimpulan; bahwa terdapat perbedaan bermakna penyembuhan luka post hecting antara ekstrak WJMSC dengan D gel


Author(s):  
Mouhamadou Wade ◽  
Mamour Gueye ◽  
Aissatou Mbodji ◽  
Mame D. Ndiaye

Wharton's jelly is a mucoid, avascular and connective tissue which plays the role of umbilical vessels protection. Its absence exposes to poor neonatal outcomes or fetal death. We report a rare case of Absence of Wharton’s Jelly, diagnosed by examination of the placenta the examination with a live fetus.


2021 ◽  
Author(s):  
Beilei Ma ◽  
Tengkai Wang ◽  
Juan Li ◽  
Qian Wang

Abstract Background Angiogenesis is required in many physiological conditions, including bone regeneration, wound healing, and tissue regeneration. Cell-derived extracellular matrix (CD-ECM) could guide intricate cellular and tissue processes such as homeostasis, healing and regeneration. Methods The purpose of this study is to explore the effect and mechanism of ECM derived from decellularized Wharton's Jelly-derived mesenchymal stem cells (WJ-MSCs) on endothelial cell viability and angiogenesis. Results In this study, we found for the first time that WJ-MSCs ECM could improve the angiogenesis ability of human umbilical vein endothelial cells (HUVECs) with a time-dependent manner in vitro. Mechanically, WJ-MSCs ECM activated the focal adhesion kinase (FAK)/P38 signaling pathway via integrin αVβ3, which further promoted the expression of the cellular (c)-Myc. Further, c-Myc increased histone acetylation levels of the vascular endothelial growth factor (VEGF) promoter by recruiting P300, which ultimately promoting VEGF expression. Conclusions Extracellular matrix derived from Wharton’s Jelly-derived mesenchymal stem cells promotes angiogenesis via integrin αVβ3/c-Myc/P300/VEGF. This study is expected to provide a new approach to promote angiogenesis in bone and tissue regeneration.


2021 ◽  
Author(s):  
Mahnaz Tashakori ◽  
Fatemeh Asadi ◽  
Faezeh-Sadat Khorram ◽  
Azita Manshoori ◽  
Ali Hosseini-Chegeni ◽  
...  

Abstract BackgroundMesenchymal stem cells (MSCs), derived from various tissues, have served as a promising source of cells in clinic and regenerative medicine. Umbilical cord-Wharton’s jelly (WJ-MSCs)-derived MSCs exhibit advantages over those from adult tissues, such as no ethical concerns, shorter population doubling time, broad differentiation potential, readily available non-invasive source, prolonged maintenance of stemness properties. Material and methodsThe aim of this study was to evaluate the effect of MRI (1.5 T, 10 min) on stemness gene expression patterns (OCT-4, SOX-2, NANOG) of WJ-MSCs. In addition, we assessed cell viability, growth kinetics and apoptosis of WJ-MSCs after MRI treatment. ResultsThe quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) data showed that transcript levels of SOX-2, NANOG in MRI-treated WJ-MSCs were increased 32- and 213-fold, respectively. MTT assay was performed at 24, 48, and 72 hours post-treatment and the viability was not significantly difference between two groups. The doubling time of MRI group was markedly higher than control group. In addition, the colony formation ability of WJ-MSCs after MRI treatment significantly increased. Furthermore, no change in apoptosis was seen before or after MRI treatment. ConclusionsOur results suggest the use of MRI can improve quality of MSCs and may enhance the efficacy of mesenchymal stem cell-based therapies.


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