Identification and characterization of an Nrf2-mediated ARE upstream of the rat glutamate cysteine ligase catalytic subunit gene (GCLC)

Glucose-6-phosphatase (G6Pase) catalyzes the final step in the gluconeogenic and glycogenolytic pathways, the hydrolysis of glucose-6-phosphate (G6P) to glucose and phosphate. This paper describes the identification and characterization of a cDNA and the gene encoding the mouse ubiquitously expressed G6Pase catalytic subunit-related protein (UGRP). The open reading frame of this UGRP cDNA encodes a protein (346 amino acids (aa); Mr 38,755) that shares 36% overall identity (56% similarity) with the mouse G6Pase catalytic subunit (357 aa; Mr 40,454). UGRP exhibits a similar predicted transmembrane topology and conservation of many of the catalytically important residues with the G6Pase catalytic subunit; however, unlike the G6Pase catalytic subunit, UGRP does not catalyze G6P hydrolysis and does not contain a carboxy-terminal di-lysine endoplasmic reticulum retention signal. UGRP mRNA was detected by RNA blot analysis in every mouse tissue examined with the highest expression in heart, brain, testis and kidney. Database analysis showed that the mouse UGRP gene is composed of six exons, spans approximately 4.2 kbp of genomic DNA and is located on chromosome 11 along with the G6Pase catalytic subunit gene. The UGRP gene transcription start sites were mapped by primer extension analysis, and the activity of the mouse UGRP gene promoter was analyzed using luciferase fusion gene constructs. In contrast to the G6Pase catalytic subunit gene promoter, the UGRP promoter was highly active in all cell lines examined.

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Identification and characterization of a human cDNA and gene encoding a ubiquitously expressed glucose-6-phosphatase catalytic subunit-related protein

Journal of Molecular Endocrinology ◽

10.1677/jme.0.0290205 ◽

2002 ◽

Vol 29 (2) ◽

pp. 205-222 ◽

Cited By ~ 60

Author(s):

CC Martin ◽

JK Oeser ◽

CA Svitek ◽

SI Hunter ◽

JC Hutton ◽

...

Keyword(s):

Fusion Gene ◽

Gene Promoter ◽

Catalytic Subunit ◽

Related Protein ◽

Subunit Gene ◽

Gene Encoding ◽

Human Cdna ◽

Highly Active ◽

Identification And Characterization

Glucose-6-phosphatase (G6Pase) catalyzes the final step in the gluconeogenic and glycogenolytic pathways, the hydrolysis of glucose-6-phosphate (G6P) to glucose and phosphate. This paper describes the identification and characterization of a human cDNA and gene encoding a ubiquitously expressed G6Pase catalytic subunit-related protein (UGRP). The ORF of this UGRP cDNA encodes a protein (346 amino acids (aa); M(r) 38 709) which shares 36% overall identity to the human G6Pase catalytic subunit (357 aa; M(r) 40 487). UGRP exhibits a similar predicted transmembrane topology and conservation of many of the catalytically important residues with the G6Pase catalytic subunit; however, unlike the G6Pase catalytic subunit, UGRP does not catalyze G6P hydrolysis. UGRP mRNA was detected by RNA blot analysis in every tissue examined with the highest expression in muscle. Database analysis showed that the human UGRP gene is composed of six exons, spans approximately 5.4 kbp of genomic DNA and is located on chromosome 17q21 with the G6Pase catalytic subunit gene. The UGRP gene transcription start sites were mapped by primer extension analysis, and the activity of the UGRP gene promoter was analyzed using luciferase fusion gene constructs. In contrast to the G6Pase catalytic subunit gene promoter, the UGRP promoter was highly active in all cell lines examined.

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Identification and Characterization of a Functional Promoter Region in the Human Eosinophil IL-5 Receptor Subunit Gene

Journal of Biological Chemistry ◽

10.1074/jbc.270.3.1462 ◽

1995 ◽

Vol 270 (3) ◽

pp. 1462-1471 ◽

Cited By ~ 35

Author(s):

Zijie Sun ◽

Donald A. Yergeau ◽

Tania Tuypens ◽

Jan Tavernier ◽

Cassandra C. Paul ◽

...

Keyword(s):

Promoter Region ◽

Receptor Subunit ◽

Subunit Gene ◽

Human Eosinophil ◽

Identification And Characterization

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Interaction of GAG trinucleotide repeat and C−129T polymorphisms impairs expression of the glutamate–cysteine ligase catalytic subunit gene

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2010.12.002 ◽

2011 ◽

Vol 50 (5) ◽

pp. 617-623 ◽

Cited By ~ 6

Author(s):

Christophe Butticaz ◽

René Gysin ◽

Michel Cuénod ◽

Kim Q. Do

Keyword(s):

Trinucleotide Repeat ◽

Catalytic Subunit ◽

Subunit Gene ◽

Glutamate Cysteine Ligase

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Cloning and characterization of the 5′-flanking region of the rat glutamate-cysteine ligase catalytic subunit

Biochemical Journal ◽

10.1042/0264-6021:3570447 ◽

2001 ◽

Vol 357 (2) ◽

pp. 447 ◽

Cited By ~ 28

Author(s):

Heping YANG ◽

Jiaohong WANG ◽

Zong-Zhi HUANG ◽

Xiaopeng OU ◽

Shelly C. LU

Keyword(s):

Catalytic Subunit ◽

Glutamate Cysteine Ligase ◽

Flanking Region

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Associations between glutamate cysteine ligase catalytic subunit gene polymorphisms and clinical characteristics of ischemic stroke

Bulletin of Russian State Medical University ◽

10.24075/brsmu.2021.007 ◽

2021 ◽

Author(s):

YA Bocharova

Keyword(s):

Ischemic Stroke ◽

Age At Onset ◽

Clinical Manifestations ◽

Lesion Size ◽

Catalytic Subunit ◽

Nucleotide Polymorphisms ◽

Cerebral Lesion ◽

Subunit Gene ◽

Glutamate Cysteine Ligase ◽

Brain Tissue Damage

An imbalance between the production of reactive oxygen species and their neutralization lies at the core of oxidative stress implicated in ischemic stroke (IS) and the subsequent brain tissue damage. The aim of this study was to investigate the effects of common polymorphic variants of the glutamate cysteine ligase catalytic subunit gene on the extent of brain damage and clinical manifestations in patients with ischemic stroke. A total of 589 ischemic stroke survivors were genotyped for 6 single nucleotide polymorphisms (SNPs) of the GCLC gene, including rs12524494, rs17883901, rs606548, rs636933, rs648595 and rs761142, using a MassARRAY-4 analyzer. The study found that genotypes rs636933-G/A-A/A (р = 0.009) and rs761142-A/C-C/C (р = 0.015) were associated with an enlargement of the cerebral lesion size. Genotypes rs12524494-G/G (р = 0.05) and rs606548-T/T (р = 0.003) were associated with a risk of 2 or more IS episodes. Genotype rs17883901-G/A was associated with early onset of IS (р = 0.004). The study revealed multiple associations of GCLC SNPs with the clinical manifestations of ischemic stroke. Thus, GCLC polymorphisms are important DNA markers affecting the size of the cerebral lesion in patients with ischemic stroke and are associated with age at onset, the number of past strokes and the clinical manifestations of the disease.

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