Cloning and sequence determination of bovine insulin-like growth factor binding protein-2 (IGFBP-2): Comparison of its structral and functional properties with IGFBP-1

1992 ◽  
Vol 48 (2) ◽  
pp. 215-226 ◽  
Author(s):  
Walker H. Busby ◽  
Robert H. McCusker ◽  
David R. Clemmons ◽  
Maureen J. Bourner ◽  
Ned R. Siegel ◽  
...  
Keyword(s):  
Growth Factor ◽  
Functional Properties ◽  
Binding Protein ◽  
Bovine Insulin ◽  
Insulin Like Growth Factor ◽  
Sequence Determination ◽  
Factor Binding

Characterization and cloning of a bovine insulin-like growth factor-binding protein

1990 ◽  
Vol 5 (1) ◽  
pp. 77-84 ◽  
Author(s):  
F. Z. Upton ◽  
L. Szabo ◽  
J. C. Wallace ◽  
F. J. Ballard
Keyword(s):  
Amino Acids ◽  
Growth Factor ◽  
Binding Protein ◽  
Sequence Similarity ◽  
Bovine Insulin ◽  
Insulin Like Growth Factor ◽  
Protein Precursor ◽  
Factor Binding ◽  
Bovine Cell ◽  
Glycosylation Sites

ABSTRACT The primary structure of the insulin-like growth factor-binding protein (IGFBP) produced by the bovine kidney cell line, MDBK, has been deduced from the cDNA clone. The MDBK binding protein precursor consists of a hydrophobic pre-peptide of at least 26 amino acids and a mature protein of 284 amino acids. The predicted protein sequence shares extensive sequence similarity with both the rat (82%) and human (89%) IGFBP-2s, so that the MDBK binding protein is clearly the bovine counterpart of IGFBP-2. The protein has limited similarity with classes 1 (31%) and 3 (31%) human IGFBPs, except that all 18 cysteine residues are conserved. Other features deduced from the bovine IGFBP-2 cDNA include: an abundance of leucine in the pre-peptide, an Arg-Gly-Asp sequence, absence of N-linked glycosylation sites, and an imperfect polyadenylation signal as well as an ATTTA motif in the 3′ non-coding DNA. Western blotting indicated that this binding protein is widely distributed in bovine fluids as well as in media conditioned by bovine cell lines. Proteins immunologically related to bovine IGFBP-2 were detected not only in sheep, but also in chickens, indicating that this IGFBP is not exclusively mammalian.

scholarly journals Bovine Insulin-Like Growth Factor Binding Protein-3: Organization of the Chromosomal Gene and Functional Analysis of Its Promoter

Endocrinology ◽  
1997 ◽  
Vol 138 (7) ◽  
pp. 2856-2862 ◽  
Author(s):  
N. E. Erondu ◽  
B. Toland ◽  
M. Boes ◽  
B. Dake ◽  
D. R. Moser ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Growth Factor ◽  
Binding Protein ◽  
Bovine Insulin ◽  
Insulin Like Growth Factor ◽  
Chromosomal Gene ◽  
Factor Binding

scholarly journals Identification of Novel SNPs in Bovine Insulin-like Growth Factor Binding Protein-3 (IGFBP3) Gene

2005 ◽  
Vol 18 (1) ◽  
pp. 3-7 ◽  
Author(s):  
J. Y. Kim ◽  
D. H. Yoon ◽  
B. L. Park ◽  
L. H. Kim ◽  
K. J. Na ◽  
...  
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Bovine Insulin ◽  
Insulin Like Growth Factor ◽  
Factor Binding

scholarly journals The Insulin-like Growth Factor (IGF) Binding Site of Bovine Insulin-like Growth Factor Binding Protein-2 (bIGFBP-2) Probed by Iodination

1996 ◽  
Vol 271 (48) ◽  
pp. 30529-30536 ◽  
Author(s):  
Graham D. Hobba ◽  
Briony E. Forbes ◽  
Emma J. Parkinson ◽  
Geoffrey L. Francis ◽  
John C. Wallace
Keyword(s):  
Growth Factor ◽  
Binding Site ◽  
Binding Protein ◽  
Bovine Insulin ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Igf Binding

Structural and Biological Characterization of Bovine Insulin-Like Growth Factor Binding Protein-3*

Endocrinology ◽  
1990 ◽  
Vol 127 (6) ◽  
pp. 2795-2803 ◽  
Author(s):  
CHERYL A. CONOVER ◽  
MICHAEL RONK ◽  
FERNANDO LOMBANA ◽  
DAVID R. POWELL
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Bovine Insulin ◽  
Insulin Like Growth Factor ◽  
Biological Characterization ◽  
Factor Binding

scholarly journals Alanine Screening Mutagenesis Establishes Tyrosine 60 of Bovine Insulin-like Growth Factor Binding Protein-2 as a Determinant of Insulin-like Growth Factor Binding

1998 ◽  
Vol 273 (31) ◽  
pp. 19691-19698 ◽  
Author(s):  
Graham D. Hobba ◽  
Agneta Löthgren ◽  
Erland Holmberg ◽  
Briony E. Forbes ◽  
Geoffrey L. Francis ◽  
...  
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Bovine Insulin ◽  
Insulin Like Growth Factor ◽  
Factor Binding

Cloning and characterization of a cDNA encoding the bovine insulin-like growth factor binding protein 1 (blGFBP-1)

DNA Sequence ◽  
1991 ◽  
Vol 1 (6) ◽  
pp. 407-408 ◽  
Author(s):  
M. Sneyers ◽  
R. Kettmann ◽  
S. Massart ◽  
R. Renaville ◽  
A. Burny ◽  
...  
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Bovine Insulin ◽  
Insulin Like Growth Factor ◽  
Factor Binding
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