The dependence of DNA synthesis on protein synthesis in HeLa S3 cells

1969 ◽  
Vol 73 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Barbara G. Weiss
Keyword(s):  
Protein Synthesis ◽  
Dna Synthesis ◽  
Hela S3 ◽  
Hela S3 Cells

scholarly journals Modification of X-Ray-Induced Killing of HeLa S3 Cells by Inhibitors of DNA Synthesis

1967 ◽  
Vol 7 (6) ◽  
pp. 779-795 ◽  
Author(s):  
Barbara G. Weiss ◽  
L.J. Tolmach
Keyword(s):  
Dna Synthesis ◽  
X Ray ◽  
Hela S3 ◽  
Hela S3 Cells

scholarly journals NUCLEAR ENVELOPE-ASSOCIATED RESUMPTION OF RNA SYNTHESIS IN LATE MITOSIS OF HELA CELLS

1973 ◽  
Vol 59 (1) ◽  
pp. 150-164 ◽  
Author(s):  
T. Simmons ◽  
P. Heywood ◽  
L. Hodge
Keyword(s):  
Protein Synthesis ◽  
Nuclear Envelope ◽  
Rna Synthesis ◽  
Electron Microscope Autoradiography ◽  
Molecular Synthesis ◽  
Hela S3 ◽  
Precursor Rna ◽  
Ribosomal Precursor ◽  
Hela S3 Cells ◽  
Silver Grains

The restitution of RNA synthesis in cultures progressing from metaphase into interphase (G1) has been investigated in synchronized HeLa S3 cells by using inhibitors of macro-molecular synthesis and the technique of electron microscope autoradiography. The rate of incorporation of radioactive uridine into RNA approached interphase levels in the absence of renewed protein synthesis. In contrast, maintenance of this rate in G1 was dependent upon renewed protein synthesis. Restoration of synthesis of heterogeneous nuclear RNA occurred under conditions that inhibited production of ribosomal precursor RNA. In autoradiographs of individual cells exposed to radioactive uridine, silver grains were first detected after nuclear envelope reformation at the periphery of the chromosome mass but before chromosomal decondensation. These data are consistent with the following interpretation. Multiple RNA polymerase activities persist through mitosis and are involved in the initiation of RNA synthesis in early telophase at sites on the nuclear envelope.

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