Age-Dependence of the X-Ray-Induced Deficiency in DNA Synthesis in HeLa S3 Cells during Generation 1

1975 ◽  
Vol 63 (3) ◽  
pp. 501 ◽  
Author(s):  
T. Dan Griffiths ◽  
L. J. Tolmach
Keyword(s):  
Dna Synthesis ◽  
Age Dependence ◽  
X Ray ◽  
Hela S3 ◽  
Hela S3 Cells

scholarly journals Modification of X-Ray-Induced Killing of HeLa S3 Cells by Inhibitors of DNA Synthesis

1967 ◽  
Vol 7 (6) ◽  
pp. 779-795 ◽  
Author(s):  
Barbara G. Weiss ◽  
L.J. Tolmach
Keyword(s):  
Dna Synthesis ◽  
X Ray ◽  
Hela S3 ◽  
Hela S3 Cells

X-ray microanalysis of HeLa S3 cells. II. Analysis of elemental levels during the cell cycle

1983 ◽  
Vol 62 (1) ◽  
pp. 339-350
Author(s):  
A. Warley ◽  
J. Stephen ◽  
A. Hockaday ◽  
T.C. Appleton
Keyword(s):  
Cell Cycle ◽  
Monovalent Cation ◽  
The Other ◽  
G1 Phase ◽  
Sodium Potassium ◽  
X Ray ◽  
Cation Concentration ◽  
Cell Compartments ◽  
Hela S3 ◽  
Hela S3 Cells

HeLa S3 cells were synchronized using hydroxyurea. Cryoultramicrotomy and X-ray microanalysis were used to study changes occurring in concentrations of elements during the cell cycle of the synchronized cells. Three subcellular compartments were studied: cytoplasm, nucleus and nucleolus. Potassium concentrations showed little fluctuation in all of the cell compartments during the cell cycle. Sodium concentrations increased during S. and M phases, returning to lower levels in the G1 phase. Chlorine concentrations were highest during the S and G2 phases. At all stages of the cell cycle respective concentrations of potassium, sodium, sulphur and chlorine were similar in the cytoplasm and nucleus. Concentrations of phosphorus increased in the nucleus during S, G2 and M, and also showed fluctuations in the nucleolus during the cycle; these were not seen in the cytoplasm. In S, M and M/G1 sodium concentrations were highest in the nucleolus compared with the other compartments. In the cytoplasm these changes resulted in an increase in total monovalent cation concentration (i.e. sodium + potassium) during S, G2 and M, which returned to base levels after mitosis. This increase in monovalent cation concentration is due almost entirely to the increase in sodium, with little change occurring in the concentration of potassium.

Susceptibility of X-Ray-Arrested HeLa S3 Cells to Additional Arrest

1976 ◽  
Vol 66 (3) ◽  
pp. 649 ◽  
Author(s):  
L. J. Tolmach ◽  
T. D. Griffiths ◽  
R. W. Jones
Keyword(s):  
X Ray ◽  
Hela S3 ◽  
Hela S3 Cells

X-ray microanalysis of HeLa S3 Cells. I. Instrumental calibration and analysis of randomly growing cultures

1983 ◽  
Vol 60 (1) ◽  
pp. 217-229
Author(s):  
A. Warley ◽  
J. Stephen ◽  
A. Hockaday ◽  
T.C. Appleton
Keyword(s):  
Cell Damage ◽  
Linear Calibration ◽  
Photometric Analysis ◽  
Sodium Potassium ◽  
X Ray ◽  
Potassium Tartrate ◽  
Freeze Dried ◽  
Hela S3 ◽  
Low K ◽  
Hela S3 Cells

Cryo-ultramicrotomy and X-ray microanalysis were used to study the elemental composition of HeLa S3 cells. Quantitation was achieved by reference to elemental standards of known concentration made up in 25% gelatin. Analysis of standards showed linear calibration for each of the elements studied: Na, P, S, Cl, K. Standardization was validated by comparing flame-photometric analysis of gelatin containing sodium potassium tartrate with that of X-ray microanalysis. Freeze-dried sections of cells showed good morphology and analysis of whole sections of the cells showed that K/Na varied in individual cells. Low K/Na could not be ascribed to cell damage or to the sequestering of Na in any particular subcompartment of the cells. Treatment with ouabain caused changes in levels of all the elements studied and resulted in a low K/Na ratio in all cells.

Dependence of the Rate of DNA Synthesis in X-Irradiated HeLa S3 Cells on Dose and Time after Exposure

1977 ◽  
Vol 69 (1) ◽  
pp. 117 ◽  
Author(s):  
L. J. Tolmach ◽  
R. W. Jones
Keyword(s):  
Dna Synthesis ◽  
Hela S3 ◽  
Hela S3 Cells
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