Analysis of the induction and deinduction of tyrosine aminotransferase in enucleated HTC cells

1975 ◽  
Vol 85 (S1) ◽  
pp. 357-364 ◽  
Author(s):  
R. D. Ivarie ◽  
W. J.-W. Fan ◽  
G. M. Tomkins
Keyword(s):  
Tyrosine Aminotransferase ◽  
Htc Cells

REGULATION OF TYROSINE AMINOTRANSFERASE mRNA IN HTC CELLS*

1980 ◽  
Vol 349 (1) ◽  
pp. 183-194 ◽  
Author(s):  
Daryl Granner ◽  
Pamela Olson ◽  
Sarah Seifert ◽  
Charlotte Block ◽  
Martin Diesterhaft ◽  
...  
Keyword(s):  
Tyrosine Aminotransferase ◽  
Htc Cells

scholarly journals Tyrosine aminotransferase sensitivity to bromodeoxyuridine during restricted intervals of S phase in hepatoma cells.

1980 ◽  
Vol 87 (3) ◽  
pp. 629-632 ◽  
Author(s):  
J C O'Brien
Keyword(s):  
Tissue Culture ◽  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Rna Synthesis ◽  
Tyrosine Aminotransferase ◽  
S Phase ◽  
Protein And Rna Synthesis ◽  
Htc Cells ◽  
Hepatoma Tissue ◽  
Dna Incorporation

Synchronized hepatoma tissue culture (HTC) cells, accumulated at the G1/S boundary with aminopterin, were released into S phase with either thymidine or 5-bromodeoxyuridine (BUdR). Tyrosine aminotransferase (TAT) activity was found to be unaffected by BUdR over the initial 3 h of S phase, but then to rapidly decline to a new basal level of 40% of control by 9 h. There was no corresponding response in the activities of alcohol dehydrogenase, malate dehydrogenase, acid phosphatase, and alkaline phosphatase, or in the rate of protein and RNA synthesis. If BUdR incorporation was restricted to limited periods of S phase, TAT was found to be maximally suppressed by incorporation into the initial 40% of the DNA. Incorporation of the analogue into the latter 60% of DNA synthesized during S phase had no effect on TAT. This is the first report that the effect of BUdR on TAT in HTC cells is associated with incorporation of the analog into DNA synthesized during a specific interval of S phase.

scholarly journals The Inductions of Tyrosine Aminotransferase by Glucagon and Hydrocortisone

1967 ◽  
Vol 242 (11) ◽  
pp. 2688-2692
Author(s):  
Vilmos Csányi ◽  
Olga Greengard ◽  
W. Eugene Knox
Keyword(s):  
Tyrosine Aminotransferase

scholarly journals THE INHERITANCE OF ENZYME VARIANTS FOR TYROSINE AMINOTRANSFERASE, NADP-DEPENDENT MALATE DEHYDROGENASE, NADP-DEPENDENT ISOCITRATE DEHYDROGENASE, AND TETRAZOLIUM OXIDASE IN TETRAHYMENA PYRIFORMIS, SYNGEN 1

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 595-603
Author(s):  
D Borden ◽  
E T Miller ◽  
D L Nanney ◽  
G S Whitt
Keyword(s):  
Detailed Analysis ◽  
Malate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Gel Electrophoresis ◽  
Tetrahymena Pyriformis ◽  
Tyrosine Aminotransferase ◽  
Breeding Program ◽  
Starch Gel Electrophoresis ◽  
Enzyme Locus ◽  
Starch Gel

ABSTRACT The isozymic patterns of tyrosine aminotransferase, NADP malate dehydrogenase, NADP isocitrate dehydrogenase, and tetrazolium oxidase were examined by starch-gel electrophoresis in Tetrahymena pyriformis, syngen 1. The genetics of the alleles controlling these enzymes was studied through a breeding program. Each enzyme locus was shown to assort vegetatively, as do other loci in this organism. A detailed analysis of the assortment process for the tyrosine aminotransferase locus indicated that the rate of stabilization of heterozygotes into pure types was essentially identical to previously-reported rates for other loci.

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