scholarly journals Cell dynamics in Hertwig's epithelial root sheath are regulated by β‐catenin activity during tooth root development

2020 ◽  
Author(s):  
Siqin Yang ◽  
Hwajung Choi ◽  
Tak‐Heun Kim ◽  
Ju‐Kyung Jeong ◽  
Yudong Liu ◽  
...  
Keyword(s):  
Root Development ◽  
Tooth Root ◽  
Cell Dynamics ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath

Sonic Hedgehog Signaling is Important in Tooth Root Development

2006 ◽  
Vol 85 (5) ◽  
pp. 427-431 ◽  
Author(s):  
M. Nakatomi ◽  
I. Morita ◽  
K. Eto ◽  
M.S. Ota
Keyword(s):  
Sonic Hedgehog ◽  
Root Development ◽  
Mutant Cell ◽  
Expression Patterns ◽  
Nuclear Antigen ◽  
Tooth Root ◽  
Shh Signaling ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath

Hertwig’s epithelial root sheath (HERS) is important for tooth root formation, but the molecular basis for the signaling of root development remains uncertain. We hypothesized that Sonic hedgehog (Shh) signaling is involved in the HERS function, because it mediates epithelial-mesenchymal interactions during embryonic odontogenesis. We examined the gene expression patterns of Shh signaling in murine developing molar roots. Shh and Patched2 transcripts were identified in the HERS, whereas Patched1, Smoothened, and Gli1 were expressed in the proliferative dental mesenchyme in addition to the HERS. To confirm whether Shh signaling physiologically functions in vivo, we analyzed mesenchymal dysplasia ( mes) mice carrying an abnormal C-terminus of the PATCHED1 protein. In the mutant, cell proliferation was repressed around the HERS at 1 wk. Moreover, the molar eruption was disturbed, and all roots were shorter than those in control littermates at 4 wks. These results indicate that Shh signaling is important in tooth root development. Abbreviations used: BrdU, 5-bromo-2′-deoxyuridine; HERS, Hertwig’s epithelial root sheath; NFI-C/CTF, nuclear factor Ic/CAAT box transcription factor; PCNA, proliferating cell nuclear antigen; Ptc, patched; Shh, sonic hedgehog; Smo, smoothened.

Role of Hertwig's epithelial root sheath cells in tooth root development

2003 ◽  
Vol 228 (4) ◽  
pp. 651-663 ◽  
Author(s):  
Margarita Zeichner-David ◽  
Keiji Oishi ◽  
Zhengyan Su ◽  
Vassili Zakartchenko ◽  
Li-Sha Chen ◽  
...  
Keyword(s):  
Root Development ◽  
Tooth Root ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath ◽  
Sheath Cells

BMP4 signaling regulates formation of Hertwig’s epithelial root sheath during tooth root development

2008 ◽  
Vol 333 (3) ◽  
pp. 503-509 ◽  
Author(s):  
Akihiro Hosoya ◽  
Ji-Youn Kim ◽  
Sung-Won Cho ◽  
Han-Sung Jung
Keyword(s):  
Root Development ◽  
Tooth Root ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath

Immortalized Hertwig's epithelial root sheath cell line works as model for epithelial–mesenchymal interaction during tooth root formation

2019 ◽  
Vol 235 (3) ◽  
pp. 2698-2709 ◽  
Author(s):  
Sicheng Zhang ◽  
Xuebing Li ◽  
Shikai Wang ◽  
Yan Yang ◽  
Weihua Guo ◽  
...  
Keyword(s):  
Cell Line ◽  
Root Formation ◽  
Tooth Root ◽  
Sheath Cell ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath

Developmental Properties of the Hertwig’s Epithelial Root Sheath in Mice

2004 ◽  
Vol 83 (9) ◽  
pp. 688-692 ◽  
Author(s):  
H. Yamamoto ◽  
S.-W. Cho ◽  
E.-J. Kim ◽  
J.-Y. Kim ◽  
N. Fujiwara ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Culture System ◽  
Root Formation ◽  
Expression Patterns ◽  
Tooth Root ◽  
Comprehensive Model ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath ◽  
In Vitro Culture System

Hertwig’s epithelial root sheath (HERS) plays an important role in tooth root formation. In this study, we examined root formation of the first molar in mice, focusing on cell proliferation, cell death, cell migration, and the expression patterns of the signaling molecules, including glycoproteins and proteoglycans between PN8 and PN26. The number of HERS cells decreased during root formation, although HERS retained total length until PN15. The migration of HERS cells did not occur during root formation. Moreover, the immunopositive reaction of laminin beta-3 and syndecan-1 in HERS indicates that both cell adhesion and cell proliferation are essential for HERS development. Bmp-2, Bmp-4, and Msx-2 were expressed in HERS cells during root formation. We also developed an in vitro culture system for investigating the periodontium and suggest that this system provides an excellent vehicle for full exploration, and hence improved understanding, of the development and regeneration of the periodontium. Together, our results provide a comprehensive model describing the morphogenesis of early root development in vertebrates.

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