Separation of 3- to 6-ring polycyclic aromatic hydrocarbons by capillary gas chromatography with a liquid crystal as stationary phase

1984 ◽  
Vol 7 (11) ◽  
pp. 637-638 ◽  
Author(s):  
H. Moser ◽  
H. Arm
Keyword(s):  
Gas Chromatography ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Liquid Crystal ◽  
Stationary Phase ◽  
Aromatic Hydrocarbons ◽  
Capillary Gas Chromatography ◽  
Polycyclic Aromatic

Degradation of polycyclic aromatic hydrocarbons and aromatic heterocycles by a Pseudomonas species

1988 ◽  
Vol 34 (10) ◽  
pp. 1135-1141 ◽  
Author(s):  
J. M. Foght ◽  
D. W. S. Westlake
Keyword(s):  
Gas Chromatography ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Capillary Gas Chromatography ◽  
Pseudomonas Sp ◽  
Aromatic Heterocycles ◽  
Wide Range ◽  
Pseudomonas Species ◽  
Polycyclic Aromatic ◽  
Cryptic Plasmids

Enrichment cultures were established with the aromatic fraction of a crude oil and screened for aromatic-degrading pseudomonads, using a sprayed plate technique. One isolate identified as Pseudomonas sp. HL7b was chosen for further study because it oxidized several polycyclic aromatic hydrocarbons and aromatic heterocycles without an apparent lag. Using capillary gas chromatography, spectrophotometry, and radiorespirometry, it was found to be capable of mineralizing and (or) oxidizing a wide range of polycyclic aromatic hydrocarbons, S-, N-, and O-heterocyclic analogues, and alkyl polycyclic aromatic hydrocarbons, but not aliphatic hydrocarbons. The isolate displayed two colonial morphologies which correlated with variation in degradative phenotype and hydrophobicity as measured by polystyrene adherence. Four cryptic plasmids were observed in both colonial types. Pseudomonas sp. HL7b degraded dibenzothiophene co-metabolically by a recognized pathway, but this degradation was constitutive, rather than inducible as reported for other bacteria.

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