A Wave of elevated intracellular free calcium spreads through human neutrophils during phagocytosis of zymosan

1992 ◽  
Vol 51 (5) ◽  
pp. 437-443 ◽  
Author(s):  
J. Conrad Schwab ◽  
Denis A. Leong ◽  
Gerald L. Mandell
Keyword(s):  
Intracellular Free Calcium ◽  
Human Neutrophils ◽  
Free Calcium

scholarly journals The dynamics of exocytosis in human neutrophils.

1988 ◽  
Vol 107 (6) ◽  
pp. 2117-2123 ◽  
Author(s):  
O Nüsse ◽  
M Lindau
Keyword(s):  
Calcium Concentration ◽  
Cytochalasin B ◽  
Intracellular Free Calcium ◽  
Morphological Data ◽  
Human Neutrophils ◽  
Free Calcium ◽  
Membrane Area ◽  
Intracellular Free Calcium Concentration ◽  
Free Calcium Concentration ◽  
Gamma S

We have investigated the dynamics of exocytosis in single human neutrophils. The increase of membrane area associated with granule fusion was followed by time-resolved patch-clamp capacitance measurements. Intracellular application of 20 microM guanosine-5'-O(3-thiotriphosphate) (GTP gamma S) in the presence of 2.5 mM ATP stimulated exocytosis and led to an increase of membrane capacitance from 3.0 to integral of 8.4 pF corresponding to a 540 micron 2 increase of membrane area. This capacitance change is very close to the value expected from morphological data if all primary and secondary granules fuse with the plasma membrane. High resolution measurements revealed stepwise capacitance changes corresponding to the fusion of individual granules. GTP gamma S-stimulated exocytosis did not require pretreatment with cytochalasin B and the amplitude was independent of the intracellular-free calcium concentration between 10 nM and integral of 2.5 microM. In the absence of GTP gamma S elevation of intracellular-free calcium concentration to the micromolar range led to the fusion of only a limited number of granules. Degranulation stimulated with GTP gamma S started after a lag phase of 2-7 min and was usually complete within 5-20 min. The time course was affected by the intracellular ATP and calcium concentration. Exocytosis was markedly accelerated by pretreatment with cytochalasin B. Our results demonstrate that the final steps leading to primary and secondary granule fusion are controlled by a guanine nucleotide-binding protein and do not require an elevation of intracellular calcium. Calcium and other factors are, however, involved in the regulation having pronounced effects on the dynamics of exocytosis.

Killing of phagocytosed Staphylococcus aureus by human neutrophils requires intracellular free calcium

1996 ◽  
Vol 59 (6) ◽  
pp. 902-907 ◽  
Author(s):  
åsa Wilsson ◽  
Helen Lundqvist ◽  
Mikael Gustafsson ◽  
Olle Stendahl
Keyword(s):  
Staphylococcus Aureus ◽  
Intracellular Free Calcium ◽  
Human Neutrophils ◽  
Free Calcium

Superoxide release and intracellular free calcium of calcium-depleted human neutrophils stimulated by N-formyl-methionyl-leucyl-phenylalanine

1984 ◽  
Vol 805 (1) ◽  
pp. 97-103 ◽  
Author(s):  
Miwako Nakagawara ◽  
Koichiro Takeshige ◽  
Hideki Sumimoto ◽  
Junichi Yoshitake ◽  
Skigeki Minakami
Keyword(s):  
Intracellular Free Calcium ◽  
Human Neutrophils ◽  
Free Calcium ◽  
Superoxide Release

scholarly journals Superoxide anion increases intracellular free calcium in human myometrial cells.

1990 ◽  
Vol 265 (36) ◽  
pp. 22533-22536
Author(s):  
N Masumoto ◽  
K Tasaka ◽  
A Miyake ◽  
O Tanizawa
Keyword(s):  
Superoxide Anion ◽  
Intracellular Free Calcium ◽  
Free Calcium ◽  
Myometrial Cells

scholarly journals Spontaneous and chemoattractant-induced oscillations of cytosolic free calcium in single adherent human neutrophils.

1988 ◽  
Vol 263 (22) ◽  
pp. 10557-10560 ◽  
Author(s):  
M E Jaconi ◽  
R W Rivest ◽  
W Schlegel ◽  
C B Wollheim ◽  
D Pittet ◽  
...  
Keyword(s):  
Human Neutrophils ◽  
Free Calcium ◽  
Cytosolic Free Calcium
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