Gas-phase doubly charged complexes of cyclic peptides with copper in +1, +2 and +3 formal oxidation states: formation, structures and electron capture dissociation

2012 ◽  
Vol 47 (2) ◽  
pp. 208-220 ◽  
Author(s):  
Carlos Afonso ◽  
Jean-Claude Tabet ◽  
Gianluca Giorgi ◽  
František Tureček
Keyword(s):  
Gas Phase ◽  
Electron Capture ◽  
Electron Capture Dissociation ◽  
Cyclic Peptides ◽  
Oxidation States ◽  
Doubly Charged

Gas-Phase Structure of Amyloid-β (12 – 28) Peptide Investigated by Infrared Spectroscopy, Electron Capture Dissociation and Ion Mobility Mass Spectrometry

2013 ◽  
Vol 24 (12) ◽  
pp. 1937-1949 ◽  
Author(s):  
Thi Nga Le ◽  
Jean Christophe Poully ◽  
Frédéric Lecomte ◽  
Nicolas Nieuwjaer ◽  
Bruno Manil ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Infrared Spectroscopy ◽  
Gas Phase ◽  
Electron Capture ◽  
Electron Capture Dissociation ◽  
Ion Mobility ◽  
Phase Structure ◽  
Amyloid Β ◽  
Ion Mobility Mass Spectrometry ◽  
Gas Phase Structure

scholarly journals Electron Capture Dissociation as Structural Probe for Noncovalent Gas-Phase Protein Assemblies

2006 ◽  
Vol 78 (20) ◽  
pp. 7191-7196 ◽  
Author(s):  
Rimco B. J. Geels ◽  
Saskia M. van der Vies ◽  
Albert J. R. Heck ◽  
Ron M. A. Heeren
Keyword(s):  
Gas Phase ◽  
Electron Capture ◽  
Electron Capture Dissociation ◽  
Protein Assemblies ◽  
Structural Probe ◽  
Phase Protein

Electron capture dissociation of a self-assembled tetranuclear metallosupramolecular complex in the gas phase

2013 ◽  
Vol 354-355 ◽  
pp. 152-158 ◽  
Author(s):  
Rainer Hovorka ◽  
Marianne Engeser ◽  
Arne Lützen
Keyword(s):  
Gas Phase ◽  
Electron Capture ◽  
Electron Capture Dissociation ◽  
Self Assembled

scholarly journals Multiplexed Conformationally-Selective, Localized Gas-Phase Hydrogen Deuterium Exchange of Protein Ions Enabled by Transmission-Mode Electron Capture Dissociation

2021 ◽  
Author(s):  
Ritu Chaturvedi ◽  
Ian Webb
Keyword(s):  
Cytochrome C ◽  
Gas Phase ◽  
Charge State ◽  
Electron Capture ◽  
Electron Capture Dissociation ◽  
Ion Mobility ◽  
Transmission Mode ◽  
Hydrogen Deuterium Exchange ◽  
Protein Ions ◽  
Hydrogen Deuterium

In this article, we present an approach for conformationally multiplexed localized hydrogen deuterium exchange (HDX) of gas-phase protein ions facilitated by ion mobility (IM) followed by electron capture dissociation (ECD). A quadrupole-ion mobility-time of flight instrument previously modified to enable ECD in transmission mode (without ion trapping) immediately following a mobility separation was further modified to allow for deuterated ammonia (ND3) to be leaked in after m/z selection. Collisional activation was minimized to prevent deuterium scrambling from giving structurally irrelevant results. This arrangement was demonstrated with the extensively studied protein folding models ubiquitin and cytochrome c. Ubiquitin was ionized from conditions that stabilize the native state and conditions that stabilize the partially-folded A-state. IM of deuterated ubiquitin 6+ ions allowed the separation of more compact conformers from more extended conformers. ECD of the separated subpopulations revealed that the more extended (later arriving) conformers had significant, localized differences in the amount of HDX observed. The 5+ charge state showed greater protection against HDX than the compact 6+ conformer, and the 11+ charge state, ionized from conditions that stabilize the A-state, showed much greater deuterium incorporation. The 7+ ions of cytochrome c ionized from aqueous conditions showed greater HDX with exterior and more unstructured regions of the protein, while interior, structured regions, especially those involved in heme binding, were more protected against exchange. These results, as well as potential future methods and experiments, are discussed herein.

scholarly journals Probing Protein Structure and Folding in the Gas Phase by Electron Capture Dissociation

2015 ◽  
Vol 26 (7) ◽  
pp. 1059-1067 ◽  
Author(s):  
Moritz Schennach ◽  
Kathrin Breuker
Keyword(s):  
Protein Structure ◽  
Gas Phase ◽  
Electron Capture ◽  
Electron Capture Dissociation

Structural Interrogation of Electrosprayed Peptide Ions by Gas-Phase H/D Exchange and Electron Capture Dissociation Mass Spectrometry

2011 ◽  
Vol 84 (1) ◽  
pp. 373-378 ◽  
Author(s):  
Jingxi Pan ◽  
Brittany L. Heath ◽  
Rebecca A. Jockusch ◽  
Lars Konermann
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Electron Capture ◽  
Electron Capture Dissociation ◽  
Peptide Ions
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