Impact of nanoparticles on transcriptional regulation of catabolic genes of petroleum hydrocarbon-degrading bacteria in contaminated soil microcosms

2018 ◽  
Vol 59 (2) ◽  
pp. 166-180
Author(s):  
Wael S. El-Sayed ◽  
Yasser Elbahloul ◽  
Mohamed E. Saad ◽  
Ahmed M. Hanafy ◽  
Abdelrahman H. Hegazi ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Soil Microcosms ◽  
Catabolic Genes ◽  
Degrading Bacteria

scholarly journals Bioremediation Experiment Using Hydrocarbon Degrading Bacteria

2019 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Rita Susilawati
Keyword(s):  
Laboratory Experiment ◽  
Contaminated Soil ◽  
Microscopic Observation ◽  
Petroleum Hydrocarbon ◽  
Beach Sand ◽  
Degrading Bacteria ◽  
Set Up

A laboratory experiment was set up to demonstrate the capability of microbe to remediate petroleum hydrocarbon contaminated beach sand. Oil contaminated soil was used as a source of inoculum for hydrocarbon degrading bacteria (HDB) while oil contaminated beach sand was used as remediation object. The growth of HDB in the inocula was enriched and stimulated through the addition of nutrient in the form of vitamin and mineral as well the addition of oil waste as a source of carbon. Experiment took place in the course of approximately five weeks. Microscopic observation clearly showed the interaction between microbe and oil contaminant both in enrichment and bioremediation samples. The result of the experiment also suggests that approximately 25% of the petroleum hydrocarbon mass in the contaminated beach sand was biodegraded over the course of one month. Overall, the results of this experiment suggest the potential of bioremediation method to treat petroleum hydrocarbon polluted environment.Keywords: bacteria, bioremediation, hydrocarbon DOI: 10.33332/jgsm.2019.v20.1.1-7

scholarly journals Bioremediation of petroleum hydrocarbon-contaminated soil by petroleum-degrading bacteria immobilized on biochar

RSC Advances ◽  
2019 ◽  
Vol 9 (60) ◽  
pp. 35304-35311 ◽  
Author(s):  
Bofan Zhang ◽  
Liang Zhang ◽  
Xiuxia Zhang
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Soil Ecosystem ◽  
Degrading Bacteria

The immobilization of bacteria on biochar was effective in reducing TPHs, n-alkanes with C12–18 and maintaining the balance of the soil ecosystem.

scholarly journals Bioremediation Experiment Using Hydrocarbon Degrading Bacteria

2019 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Dr. Rita Susilawati
Keyword(s):  
Laboratory Experiment ◽  
Contaminated Soil ◽  
Microscopic Observation ◽  
Petroleum Hydrocarbon ◽  
Beach Sand ◽  
Degrading Bacteria ◽  
Set Up

A laboratory experiment was set up to demonstrate the capability of microbe to remediate petroleum hydrocarbon contaminated beach sand. Oil contaminated soil was used as a source of inoculum for hydrocarbon degrading bacteria (HDB) while oil contaminated beach sand was used as remediation object. The growth of HDB in the inocula was enriched and stimulated through the addition of nutrient in the form of vitamin and mineral as well the addition of oil waste as a source of carbon. Experiment took place in the course of approximately five weeks. Microscopic observation clearly showed the interaction between microbe and oil contaminant both in enrichment and bioremediation samples. The result of the experiment also suggests that approximately 25% of the petroleum hydrocarbon mass in the contaminated beach sand was biodegraded over the course of one month. Overall, the results of this experiment suggest the potential of bioremediation method to treat petroleum hydrocarbon polluted environment.Keywords: bacteria, bioremediation, hydrocarbon

scholarly journals Bioremediation Potential of Native Hydrocarbons Degrading Bacteria in Crude Oil Polluted Soil

2017 ◽  
Vol 74 (1) ◽  
pp. 19 ◽  
Author(s):  
Mariana MARINESCU ◽  
Anca LACATUSU ◽  
Eugenia GAMENT ◽  
Georgiana PLOPEANU ◽  
Vera CARABULEA
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Polluted Soil ◽  
Total Petroleum Hydrocarbon ◽  
Polluted Soils ◽  
Degrading Bacteria ◽  
Bacterial Inoculum ◽  
Native Crude ◽  
Petroleum Pollutants

Bioremediation of crude oil contaminated soil is an effective process to clean petroleum pollutants from the environment. Crude oil bioremediation of soils is limited by the bacteria activity in degrading the spills hydrocarbons. Native crude oil degrading bacteria were isolated from different crude oil polluted soils. The isolated bacteria belong to the genera Pseudomonas, Mycobacterium, Arthrobacter and Bacillus. A natural biodegradable product and bacterial inoculum were used for total petroleum hydrocarbon (TPH) removal from an artificial polluted soil. For soil polluted with 5% crude oil, the bacterial top, including those placed in the soil by inoculation was 30 days after impact, respectively 7 days after inoculum application, while in soil polluted with 10% crude oil,  multiplication top of bacteria was observed in the determination made at 45 days after impact and 21 days after inoculum application, showing once again how necessary is for microorganisms habituation and adaptation to environment being a function of pollutant concentration. The microorganisms inoculated showed a slight adaptability in soil polluted with 5% crude oil, but complete inhibition in the first 30 days of experiment at 10% crude oil.

scholarly journals Degradation of diesel and phenol using bacteria isolated from petroleum hydrocarbon contaminated soil

2018 ◽  
Vol 53 (1) ◽  
pp. 53-62 ◽  
Author(s):  
F Ahmed ◽  
ANM Fakhruddin ◽  
MM Kabir
Keyword(s):  
Soil Sample ◽  
Bacterial Growth ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Culture Medium ◽  
Standard Sample ◽  
Absorption Peak ◽  
Total Hydrocarbon ◽  
Degrading Bacteria ◽  
Uv Visible

In the present investigation, diesel degrading bacteria were isolated from petroleum hydrocarbon contaminated soil and evaluated their degradation potentials of diesel and phenol at various concentrations. Total hydrocarbon was identified by extracting mixed standard sample (MSS) method from the contaminated soil and scanning wavelength from 190 to 280 nm by means of UV-visible spectrophotometer. The absorption peak of MSS solution dissolved in hexane was stable at 215 nm to 230 nm. The average number of diesel degrading bacteria in the soil sample was 2.52 × 103 at 32 ºC for72 hours. One of the representative isolates was used to degrade diesel at varying amount. At 24 h of incubation, bacterial growth increased up in 20% (v/v) of diesel but in 25% (v/v) of diesel bacterial growth decreased. This isolate completely degraded 50 mg/L phenol after 96 h of incubation from the culture medium. The isolated bacteria can be used as an effective candidate for bioremediation of diesel and phenol from the hydrocarbon contaminated environments.Bangladesh J. Sci. Ind. Res.53(1), 53-62, 2018

scholarly journals Bioremediation Experiment Using Hydrocarbon Degrading Bacteria

2019 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Rita Susilawati
Keyword(s):  
Laboratory Experiment ◽  
Contaminated Soil ◽  
Microscopic Observation ◽  
Petroleum Hydrocarbon ◽  
Beach Sand ◽  
Degrading Bacteria ◽  
Set Up

A laboratory experiment was set up to demonstrate the capability of microbe to remediate petroleum hydrocarbon contaminated beach sand. Oil contaminated soil was used as a source of inoculum for hydrocarbon degrading bacteria (HDB) while oil contaminated beach sand was used as remediation object. The growth of HDB in the inocula was enriched and stimulated through the addition of nutrient in the form of vitamin and mineral as well the addition of oil waste as a source of carbon. Experiment took place in the course of approximately five weeks. Microscopic observation clearly showed the interaction between microbe and oil contaminant both in enrichment and bioremediation samples. The result of the experiment also suggests that approximately 25% of the petroleum hydrocarbon mass in the contaminated beach sand was biodegraded over the course of one month. Overall, the results of this experiment suggest the potential of bioremediation method to treat petroleum hydrocarbon polluted environment.Keywords: bacteria, bioremediation, hydrocarbon DOI: 10.33332/jgsm.2019.v20.1.1-7

Isolation of Petroleum Hydrocarbon Degrading Bacteria from the Liaohe Estuary at Cold Climate

2015 ◽  
Vol 1092-1093 ◽  
pp. 878-881
Author(s):  
Ping Guo ◽  
Jian Guo Lin ◽  
Bin Xia Cao ◽  
Na Ta
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Degradation Rate ◽  
Diesel Oil ◽  
Cold Climate ◽  
Hydrocarbon Degradation ◽  
Oil Degradation ◽  
Degrading Bacteria ◽  
Cold Tolerant ◽  
Diesel Oil Degradation

Two cold-tolerant petroleum hydrocarbon degrading bacteria strain named CHD1 and CHD2 were isolated from oil-contaminated soil at cold climate. The isolated strains were able use diesel oil as sole carbon. The petroleum hydrocarbon degradation rate was analyzed using UV-spectrometry-based methods. The results showed that the diesel oil degradation rate of CHD1 and CHD2 were 22% and 25%, respectively.

scholarly journals Identification of Alkane Degrading Bacteria in Petroleum Hydrocarbon-Contaminated Soil

2006 ◽  
Vol 29 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Makiko KARUBE ◽  
Kazuaki SYUTSUBO ◽  
Shunji OYA ◽  
Shin TANIGUCHI ◽  
Akiko MIYA
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Degrading Bacteria

scholarly journals Bioremediation Experiment Using Hydrocarbon Degrading Bacteria

2019 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Dr. Rita Susilawati
Keyword(s):  
Laboratory Experiment ◽  
Contaminated Soil ◽  
Microscopic Observation ◽  
Petroleum Hydrocarbon ◽  
Beach Sand ◽  
Degrading Bacteria ◽  
Set Up

A laboratory experiment was set up to demonstrate the capability of microbe to remediate petroleum hydrocarbon contaminated beach sand. Oil contaminated soil was used as a source of inoculum for hydrocarbon degrading bacteria (HDB) while oil contaminated beach sand was used as remediation object. The growth of HDB in the inocula was enriched and stimulated through the addition of nutrient in the form of vitamin and mineral as well the addition of oil waste as a source of carbon. Experiment took place in the course of approximately five weeks. Microscopic observation clearly showed the interaction between microbe and oil contaminant both in enrichment and bioremediation samples. The result of the experiment also suggests that approximately 25% of the petroleum hydrocarbon mass in the contaminated beach sand was biodegraded over the course of one month. Overall, the results of this experiment suggest the potential of bioremediation method to treat petroleum hydrocarbon polluted environment.Keywords: bacteria, bioremediation, hydrocarbon

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