Effects of ZnSO4
 and Zn-EDTA applied by broadcasting or by banding on soil Zn fractions and Zn uptake by wheat (Triticum aestivum
 L.) under greenhouse conditions

Abstract Purpose Green manuring can increase the plant available fraction of zinc (Zn) in soil, making it a potential approach to increase wheat Zn concentrations and fight human Zn deficiency. We tested whether green manure increases the ability of both the native soil bacteria and inoculated Zn solubilizing bacteria (ZSB) to mobilize Zn. Methods Wheat was grown in a pot experiment with the following three factors (with or without); (i) clover addition; (ii) soil x-ray irradiation (i.e. elimination of the whole soil biota followed by re-inoculation with the native soil bacteria); and (iii) ZSB inoculation. The incorporation of clover in both the irradiated and the ZSB treatments allowed us to test green manure effects on the mobilization of Zn by indigenous soil bacteria as well as by inoculated strains. Results Inoculation with ZSB did neither increase soil Zn availability nor wheat Zn uptake. The highest soil Zn availabilities were found when clover was incorporated, particularly in the irradiated soils (containing only soil bacteria). This was partly associated with the stimulation of bacterial activity during the decomposition of the incorporated green manure. Conclusion The results support that the activity of soil bacteria is intimately involved in the mobilization of Zn following the incorporation of green manure.

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Combined Selenium and Zinc Biofortification of Bread Making Wheat under Mediterranean Conditions

10.20944/preprints202105.0368.v1 ◽

2021 ◽

Author(s):

Dolores Reynolds-Marzal ◽

Angelica Rivera-Martin ◽

Oscar Santamaria ◽

Maria J. Poblaciones

Keyword(s):

Triticum Aestivum ◽

Main Product ◽

Foliar Application ◽

Triticum Aestivum L ◽

Bread Making ◽

Main Plot ◽

Soil Zn ◽

Agronomic Biofortification ◽

Mediterranean Conditions ◽

Plot Design

Millions of people worldwide have an inadequate intake of selenium (Se) and zinc (Zn), and agronomic biofortification may minimise these problems. To evaluate the efficacy of combined foliar Se and Zn fertilisation in bread making wheat (Triticum aestivum L.), foliar Se (as NaSeO4) and soil and foliar Zn applications (as ZnSO4·7H2O) were tested individually and in all combinations. A 2-year field experiment was established in southern Spain under semiarid Mediterranean conditions, by following a split-split-plot design. The study year (2017/2018, 2018/2019) was considered as the main-plot factor, soil Zn application (50 kg Zn ha-1, nil Zn) as a subplot factor, and foliar application (nil, 10 g Se ha-1, 8 kg Zn ha-1, 10 g Se ha-1 + 8 kg Zn ha-1) as a sub-subplot factor. The best treatment to increase both Zn and Se concentration in both straw, 12.3- and 2.7-fold respectively, and grain, 1.2- and 4.1-fold respectively was the combined foliar application of Zn and Se. This combined Zn and Se application also increased on average the yield of grain, main product of this crop, by almost 26%. Therefore, bread-making wheat seems to be a very suitable crop to be used in biofortification programs with Zn and Se to alleviate their deficiency in both, people when using its grain and livestock when using its straw.

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Green Manure Effect on The Ability of Native and Inoculated Soil Bacteria to Mobilize Zinc for Wheat Uptake (Triticum Aestivum L.)

10.21203/rs.3.rs-425554/v1 ◽

2021 ◽

Author(s):

Benjamin Costerousse ◽

Joel Quattrini ◽

Roman Grüter ◽

Emmanuel Frossard ◽

Cécile Thonar

Keyword(s):

Green Manure ◽

Soil Bacteria ◽

Triticum Aestivum L ◽

Zn Deficiency ◽

X Ray ◽

Zn Uptake ◽

Native Soil ◽

Soil Zn ◽

Zn Availability ◽

Stimulation Of

Abstract PurposeGreen manuring can increase the plant available fraction of zinc (Zn) in soil, making it a potential approach to increase wheat Zn concentrations and fight human Zn deficiency. We tested whether green manure increases the ability of both the native soil bacteria and inoculated Zn solubilizing bacteria (ZSB) to mobilize Zn.MethodsWheat was grown in a pot experiment with the following three factors (with or without); (i) clover addition; (ii) soil x-ray irradiation (i.e. elimination of the whole soil biota followed by re-inoculation with the native soil bacteria); and (iii) ZSB inoculation. The incorporation of clover in both the irradiated and the ZSB treatments allowed us to test green manure effects on the mobilization of Zn by indigenous soil bacteria as well as by inoculated strains.ResultsInoculation with ZSB did neither increase soil Zn availability nor wheat Zn uptake. The highest soil Zn availabilities were found when clover was incorporated, particularly in the irradiated soils (containing only soil bacteria). This was partly associated with the stimulation of bacterial activity during the decomposition of the incorporated green manure.ConclusionThe results support that the activity of soil bacteria is intimately involved in the mobilization of Zn following the incorporation of green manure.

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