Determination of free bile acids in pharmaceutical preparations by packed column supercritical fluid chromatography

1993 ◽  
Vol 82 (1) ◽  
pp. 44-47 ◽  
Author(s):  
Santo Scalia ◽  
David E. Games
Keyword(s):  
Bile Acids ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Packed Column ◽  
Pharmaceutical Preparations ◽  
Free Bile Acids

scholarly journals Determination of Antifungals in Rodent Diet by Supercritical Fluid Extraction Followed by Packed Column Supercritical Fluid Chromatography with Ultraviolet Detection

1997 ◽  
Vol 80 (1) ◽  
pp. 7-13 ◽  
Author(s):  
John R Dean ◽  
Ian A Fowlis ◽  
Stephen M Hitchen ◽  
Sharmin Khundker ◽  
Edwin Ludkin ◽  
...  
Keyword(s):  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Mobile Phase ◽  
Packed Column ◽  
Fluid Extraction ◽  
Ultraviolet Detection ◽  
Silica Column ◽  
Column Pressure

Abstract Supercritical fluid extraction (SFE) followed by packed column supercritical fluid chromatography with ultraviolet detection was evaluated as a quantitative method for determining 4 antifungals (fluconazole, tioconazole, hexaconazole, and UK- 47,265) in rodent diet. Chromatography was achieved with a cyano-bonded silica column, UV detection at 210 nm, and methanol-modified supercritical carbon dioxide as mobile phase. The effects of modifier concentration, temperature, and column pressure on antifungal retention time was studied. Off-line SFE was optimized at 2 spike levels, ranging from 0.5 to 10 g/kg, for each of the 4 antifungals. Average recoveries ranged from 79.0% for UK-47,265 to 96.5% for hexaconazole. Overall, the procedure provides a suitable method for analyzing antifungals in spiked rodent diet.

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