Comparative studies of chemotherapy of human tumor cells in vitro by tritiated thymidine uptake inhibition and soft agar clonogenic assay

1986 ◽  
Vol 31 (2) ◽  
pp. 123-126 ◽  
Author(s):  
Karimullah A. Zirvi ◽  
George J. Hill ◽  
Helene Z. Hill
Keyword(s):  
Clonogenic Assay ◽  
Tritiated Thymidine ◽  
Human Tumor ◽  
Soft Agar ◽  
Thymidine Uptake ◽  
Human Tumor Cells ◽  
Uptake Inhibition ◽  
Tritiated Thymidine Uptake ◽  
Soft Agar Clonogenic Assay

Damage by Dichlorvos of Human Lymphocyte DNA

1980 ◽  
Vol 66 (4) ◽  
pp. 425-430 ◽  
Author(s):  
Paolo Perocco ◽  
Angela Fini
Keyword(s):  
Dna Synthesis ◽  
Human Lymphocyte ◽  
Tritiated Thymidine ◽  
Human Lymphocytes ◽  
Ultraviolet Rays ◽  
Thymidine Uptake ◽  
Short Term ◽  
In Vitro System ◽  
Tritiated Thymidine Uptake

The action of dichlorvos (2.2-dichlorovinyldimethyl phosphate) was studied with a short-term in vitro system which utilizes human lymphocytes. The parameters studied were the action exerted by the pesticide on scheduled (semiconservative) and unscheduled (reparative) DNA synthesis measured as tritiated thymidine uptake. The results obtained show that dichlorvos affects semiconservative DNA synthesis, damages human lymphocyte DNA inducing low reparative synthesis, and interferes with DNA repair processes after damage exerted by ultraviolet rays.

Comparative Sensitivity of Two Mouse Lymphoid Tumour Cell Lines (P388 and P388D1) to Five Anticancer Drugs In Vitro

1990 ◽  
Vol 18 (1_part_1) ◽  
pp. 225-230
Author(s):  
Roland Arnould ◽  
Jacque Dubois ◽  
Fokri Abikhalil ◽  
Anita Libert ◽  
Ghanem Ghanem ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Tritiated Thymidine ◽  
In Vitro Assays ◽  
Lower Sensitivity ◽  
Thymidine Uptake ◽  
Tritiated Thymidine Uptake ◽  
P388 Cell ◽  
Repeated Passage

The sensitivity and the selectivity of two murine lymphoid cell lines (P388 and P388D1) to five chemotherapeutic drugs were investigated in vitro. The cytotoxicities of melphalan, daunorubicin, hexamethylmelamine (HMM), hydroxymethylpentamethylmelamine and dihydroxymethyltetramethylmelamine, two HMM derivatives, were measured in the two cell lines using two different techniques: reduction of a tetrazolium derivative (MTT) and tritiated thymidine uptake into DNA. Cytotoxicity was expressed by the 50% inhibitory concentration (IC50) after one hour and after exposure of cells to each drug for two days. The IC50 results indicate that the P388 cells were generally more sensitive to melphalan, daunorubicin, hydroxymethylpentamethylmelamine and dihydroxymethyltetramethylmelamine than the P388D1 cell line. HMM was found to be inactive in both cell lines. Despite the lower sensitivity of the P388D1 cell line compared with the P388 cell line, because of its greater homogeneity, it could replace the P388 line for the in vitro assays, bearing in mind that the P388D1 cell line sensitivity is 1.21 - 24.63 times lower than that of the P388 cell line, at least as far as the drugs tested are concerned. Moreover, our results emphasise that variations of sensitivity could occur with repeated passage in vitro.

Toxic, DNA-Damaging and Mutagenic Activity of Epichlorohydrin on Human Cells Cultured in Vitro

1983 ◽  
Vol 69 (3) ◽  
pp. 191-194 ◽  
Author(s):  
Paolo Perocco ◽  
Paola Rocchi ◽  
Anna Maria Ferreri ◽  
Antonella Capucci
Keyword(s):  
Cell Viability ◽  
Diphtheria Toxin ◽  
Mutagenic Activity ◽  
Tritiated Thymidine ◽  
Human Lymphocytes ◽  
Metabolic Activation ◽  
Thymidine Uptake ◽  
Tritiated Thymidine Uptake ◽  
Cells Cultured

Epichlorohydrin (ECHH) highly inhibited the tritiated thymidine uptake by human lymphocytes cultured in vitro, although the corresponding cell viability was unaffected. Furthermore, it elicited unscheduled DNA synthesis, acting as a DNA-damaging agent after its metabolic activation. ECHH also showed a clear toxic and mutagenic activity toward a human epithelial-like cell line, causing a decrease in cell viability and an increase in mutants resistant to 0.05 Lf/ml of diphtheria toxin.

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