Enrichment and isolation of barbigerone fromMillettia pachycarpaBenth. using high-speed counter-current chromatography and preparative HPLC

2010 ◽  
pp. NA-NA ◽  
Author(s):  
Haoyu Ye ◽  
Shijie Zhong ◽  
Yanfang Li ◽  
Minghai Tang ◽  
Aihua Peng ◽  
...  
Author(s):  
Xue Yang ◽  
Yongling Liu ◽  
Tao Chen ◽  
Nana Wang ◽  
Hongmei Li ◽  
...  

Abstract Separation of natural compounds directly from the crude extract is a challenging work for traditional column chromatography. In the present study, an efficient method for separation of three main compounds from the crude extract of Dracocephalum tanguticum has been successfully established by high-speed counter-current chromatography (HSCCC). The crude extract was directly introduced into HSCCC by using dimethyl sulfoxide as cosolvent. Ethyl acetate/n-butyl alcohol/0.3% glacial acetic acid (4: 1: 5, v/v) system was used and three target compounds with purity higher than 80% were obtained. Preparative HPLC was used for further purification and three target compounds with purity higher than 98% were obtained. The compounds were identified as chlorogenic acid, pedaliin and pedaliin-6″-acetate.


Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2773
Author(s):  
Guanglei Zuo ◽  
Kang-Hoon Je ◽  
Yanymee N. Guillen Quispe ◽  
Kyong-Oh Shin ◽  
Hyun Yong Kim ◽  
...  

We previously reported that Lepechinia meyenii (Walp.) Epling has antioxidant and aldose reductase (AR) inhibitory activities. In this study, L. meyenii was extracted in a 50% MeOH and CH2Cl2/MeOH system. The active extracts of MeOH and 50% MeOH were subjected to fractionation, followed by separation using high-speed counter-current chromatography (HSCCC) and preparative HPLC. Separation and identification revealed the presence of caffeic acid, hesperidin, rosmarinic acid, diosmin, methyl rosmarinate, diosmetin, and butyl rosmarinate. Of these, rosmarinic acid, methyl rosmarinate, and butyl rosmarinate possessed remarkable antioxidant and AR inhibitory activities. The other compounds were less active. In particular, rosmarinic acid is the key contributor to the antioxidant and AR inhibitory activities of L. meyenii; it is rich in the MeOH extract (333.84 mg/g) and 50% MeOH extract (135.41 mg/g) of L. meyenii and is especially abundant in the EtOAc and n-BuOH fractions (373.71–804.07 mg/g) of the MeOH and 50% MeOH extracts. The results clarified the basis of antioxidant and AR inhibitory activity of L. meyenii, adding scientific evidence supporting its traditional use as an anti-diabetic herbal medicine. The HSCCC separation method established in this study can be used for the preparative separation of rosmarinic acid from natural products.


Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 230
Author(s):  
Huanzhu Zhao ◽  
Xiangyun Song ◽  
Iftikhar Ali ◽  
Manzoor Hussain ◽  
Andleeb Mehmood ◽  
...  

A silver ion high-speed counter-current chromatography ([Ag+]-HSCCC) was developed to separate and purify five sesquiterpenoids from germacrene A fermentation broth. The solvent system was consisted of n-hexane-methanol-silver nitrate (3 mol/L) solution (10:9.5:0.5, v/v). By employing this chromatographic protocol, five sesquiterpenoids named β-elemene (1; 54.1 mg), germacrene A (2; 28.5 mg), γ-selinene (3; 4.6 mg), β-selinene (4; 3.4 mg), and α-selinene (5; 1.3 mg) were obtained successfully from 500 mg extracted crude sample with purities of 97.1%, 95.2%, 98.2%, 96.3% and 98.5%, respectively, combined with preparative HPLC. The results reveal that the addition of metal ion in biphasic solvent system significantly improved the HSCCC separation factor of sesquiterpenoids. Meanwhile, our study also provided an alternate approach to separate the compounds with less polarity, also geometrical isomers and various natural product classes.


2010 ◽  
Vol 28 (8) ◽  
pp. 813-816
Author(s):  
Wei JI ◽  
Yulan CUI ◽  
Renjuan CHEN ◽  
Xinren LIN ◽  
Qingxin ZHOU ◽  
...  
Keyword(s):  

2010 ◽  
Vol 28 (4) ◽  
pp. 383-387 ◽  
Author(s):  
Aiyi PENG ◽  
Xuewei QU ◽  
Hui LI ◽  
Lu GAO ◽  
Bo YU ◽  
...  

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