Stimulated Raman Scattering Tomography Enables Label‐Free Volumetric Deep Tissue Imaging

2021 ◽  
pp. 2100069
Author(s):  
Li Gong ◽  
Shulang Lin ◽  
Zhiwei Huang
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Tissue Imaging ◽  
Label Free ◽  
Deep Tissue ◽  
Deep Tissue Imaging ◽  
Stimulated Raman

scholarly journals Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells

2019 ◽  
Vol 63 (5) ◽  
pp. 2028-2034 ◽  
Author(s):  
Kristel Sepp ◽  
Martin Lee ◽  
Marie T. J. Bluntzer ◽  
G. Vignir Helgason ◽  
Alison N. Hulme ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Intracellular Distribution ◽  
Live Cells ◽  
Label Free ◽  
Stimulated Raman

scholarly journals Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering

2019 ◽  
Vol 116 (32) ◽  
pp. 15842-15848 ◽  
Author(s):  
Yuta Suzuki ◽  
Koya Kobayashi ◽  
Yoshifumi Wakisaka ◽  
Dinghuan Deng ◽  
Shunji Tanaka ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Cancer Biology ◽  
High Speed ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Fluorescent Labeling ◽  
Label Free ◽  
Imaging Flow Cytometry ◽  
Stimulated Raman

Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural, and morphological phenotypes of numerous living cells to provide systematic insights into biological processes. However, its utility is constrained by its requirement of fluorescent labeling for phenotyping. Here we present label-free chemical imaging flow cytometry to overcome the issue. It builds on a pulse pair-resolved wavelength-switchable Stokes laser for the fastest-to-date multicolor stimulated Raman scattering (SRS) microscopy of fast-flowing cells on a 3D acoustic focusing microfluidic chip, enabling an unprecedented throughput of up to ∼140 cells/s. To show its broad utility, we use the SRS imaging flow cytometry with the aid of deep learning to study the metabolic heterogeneity of microalgal cells and perform marker-free cancer detection in blood.

scholarly journals Label-Free Quantitative Imaging of Cholesterol in Intact Tissues by Hyperspectral Stimulated Raman Scattering Microscopy

2013 ◽  
Vol 125 (49) ◽  
pp. 13280-13284 ◽  
Author(s):  
Ping Wang ◽  
Junjie Li ◽  
Pu Wang ◽  
Chun-Rui Hu ◽  
Delong Zhang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Quantitative Imaging ◽  
Label Free ◽  
Stimulated Raman

Label-Free, Real-Time Monitoring of Biomass Processing with Stimulated Raman Scattering Microscopy

2010 ◽  
Vol 49 (32) ◽  
pp. 5476-5479 ◽  
Author(s):  
Brian G. Saar ◽  
Yining Zeng ◽  
Christian W. Freudiger ◽  
Yu-San Liu ◽  
Michael E. Himmel ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Real Time ◽  
Stimulated Raman Scattering ◽  
Label Free ◽  
Real Time Monitoring ◽  
Biomass Processing ◽  
Stimulated Raman

scholarly journals Stimulated Raman scattering flow cytometry for label-free single-particle analysis

Optica ◽  
2017 ◽  
Vol 4 (1) ◽  
pp. 103 ◽  
Author(s):  
Chi Zhang ◽  
Kai-Chih Huang ◽  
Bartek Rajwa ◽  
Junjie Li ◽  
Shiqi Yang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Single Particle ◽  
Stimulated Raman Scattering ◽  
Particle Analysis ◽  
Single Particle Analysis ◽  
Label Free ◽  
Stimulated Raman
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