scholarly journals High-Speed Label-Free Spectroscopic Biological Imaging Based on Stimulated Raman Scattering MicroscopyHigh-Speed Label-Free Spectroscopic Biological Imaging Based on Stimulated Raman Scattering MicroscopyHigh-Speed Label-Free Spectroscopic Biological Imaging Based on Stimulated Raman Scattering Microscopy

2017 ◽  
Vol 45 (6) ◽  
pp. 328
Author(s):  
Yasuyuki OZEKI
2019 ◽  
Vol 116 (32) ◽  
pp. 15842-15848 ◽  
Author(s):  
Yuta Suzuki ◽  
Koya Kobayashi ◽  
Yoshifumi Wakisaka ◽  
Dinghuan Deng ◽  
Shunji Tanaka ◽  
...  

Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural, and morphological phenotypes of numerous living cells to provide systematic insights into biological processes. However, its utility is constrained by its requirement of fluorescent labeling for phenotyping. Here we present label-free chemical imaging flow cytometry to overcome the issue. It builds on a pulse pair-resolved wavelength-switchable Stokes laser for the fastest-to-date multicolor stimulated Raman scattering (SRS) microscopy of fast-flowing cells on a 3D acoustic focusing microfluidic chip, enabling an unprecedented throughput of up to ∼140 cells/s. To show its broad utility, we use the SRS imaging flow cytometry with the aid of deep learning to study the metabolic heterogeneity of microalgal cells and perform marker-free cancer detection in blood.


2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Cheng Zong ◽  
Ranjith Premasiri ◽  
Haonan Lin ◽  
Yimin Huang ◽  
Chi Zhang ◽  
...  

AbstractStimulated Raman scattering (SRS) microscopy allows for high-speed label-free chemical imaging of biomedical systems. The imaging sensitivity of SRS microscopy is limited to ~10 mM for endogenous biomolecules. Electronic pre-resonant SRS allows detection of sub-micromolar chromophores. However, label-free SRS detection of single biomolecules having extremely small Raman cross-sections (~10−30 cm2 sr−1) remains unreachable. Here, we demonstrate plasmon-enhanced stimulated Raman scattering (PESRS) microscopy with single-molecule detection sensitivity. Incorporating pico-Joule laser excitation, background subtraction, and a denoising algorithm, we obtain robust single-pixel SRS spectra exhibiting single-molecule events, verified by using two isotopologues of adenine and further confirmed by digital blinking and bleaching in the temporal domain. To demonstrate the capability of PESRS for biological applications, we utilize PESRS to map adenine released from bacteria due to starvation stress. PESRS microscopy holds the promise for ultrasensitive detection and rapid mapping of molecular events in chemical and biomedical systems.


2019 ◽  
Vol 205 ◽  
pp. 03003
Author(s):  
Francesco Saltarelli ◽  
Vikas Kumar ◽  
Daniele Viola ◽  
Francesco Crisafi ◽  
Fabrizio Preda ◽  
...  

Stimulated Raman scattering spectroscopy enables label-free molecular identification, but its broadband implementation is technically challenging. We experimentally demonstrate a novel approach to multiplex stimulated Raman scattering based on photonic time stretch. A telecom fiber stretches the broadband femtosecond Stokes pulse after the sample to ∼15ns, mapping its spectrum in time. The signal is sampled through a fast oscilloscope, providing single-shot spectra at 80-kHz rate. We demonstrate high sensitivity in detecting the Raman vibrational modes of various samples over the entire high-frequency C-H stretching region. Our results pave the way to high-speed broadband vibrational imaging for materials science and biophotonics.


Sensors ◽  
2017 ◽  
Vol 17 (11) ◽  
pp. 2581 ◽  
Author(s):  
Kamel Mars ◽  
De Xing Lioe ◽  
Shoji Kawahito ◽  
Keita Yasutomi ◽  
Keiichiro Kagawa ◽  
...  

2019 ◽  
Vol 63 (5) ◽  
pp. 2028-2034 ◽  
Author(s):  
Kristel Sepp ◽  
Martin Lee ◽  
Marie T. J. Bluntzer ◽  
G. Vignir Helgason ◽  
Alison N. Hulme ◽  
...  

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