The present study investigated the effects of Tubifex (Oligochaeta: Tubificidae) treatment on the dewatering process of mature fine tailings (MFT). Experiments testing the survival rate showed that Tubifex can survive at 20 °C and 4 °C. MFT with initial solids content (Sc) of 30% of total mass were treated in 11 settling columns by three Tubifex densities, 1400, 2000 and 4200 individuals·m−2. Test results showed that the mean survival rate at 20 °C and 4 °C on the 28th day stayed around 85%. Tubifex enhanced MFT dewatering by providing compacted tailings with 11.6% and 66.7% higher Sc and undrained shear strength compared with nontreated tailings. Tubifex accelerated pore water pressure dissipation. Tubifex did not affect the chemical composition of tailings except for a decrease in sulfate content. The Sc of tailings treated by Tubifex increased by 67.4% within nine months, which was 129% greater than the Sc increase of the nontreated tailings after 11 months.