Inhibition of hepatitis b virus surface antigen gene expression in carcinogen-induced liver tumors from transgenic mice

1994 ◽  
Vol 9 (4) ◽  
pp. 185-192 ◽  
Author(s):  
Hend Farza ◽  
Tommaso A. Dragani ◽  
Thomas Metzler ◽  
Giacomo Manenti ◽  
Pierre Tiollais ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Liver Tumors ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
Antigen Gene ◽  
B Virus

Active compounds from Saussurea lappa Clarks that suppress hepatitis B virus surface antigen gene expression in human hepatoma cells

1995 ◽  
Vol 27 (1-2) ◽  
pp. 99-109 ◽  
Author(s):  
Hua-Chien Chen ◽  
Chen-Kung Chou ◽  
Shou-Dong Lee ◽  
Ju-Chun Wang ◽  
Sheau-Farn Yeh
Keyword(s):  
Gene Expression ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
Antigen Gene ◽  
B Virus

Placebo-controlled trial of vaccination with hepatitis B virus surface antigen in hepatitis B virus transgenic mice

1997 ◽  
Vol 26 (1) ◽  
pp. 131-137 ◽  
Author(s):  
S.M. Fazle Akbar ◽  
Kazunori Kajino ◽  
Kenji Tanimoto ◽  
Kiyotaka Kurose ◽  
Toshikazu Masumoto ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Controlled Trial ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
B Virus

scholarly journals High doses of siRNAs induce eri-1 and adar-1 gene expression and reduce the efficiency of RNA interference in the mouse

2005 ◽  
Vol 390 (3) ◽  
pp. 675-679 ◽  
Author(s):  
Jie Hong ◽  
Zhikang Qian ◽  
Shuiyuan Shen ◽  
Taishan Min ◽  
Chang Tan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis B Virus ◽  
Rna Interference ◽  
Hepatitis B ◽  
Adenosine Deaminase ◽  
Surface Antigen ◽  
Target Genes ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
B Virus

RNAi (RNA interference) is a gene-silencing mechanism that is conserved in evolution from worm to human and has been a powerful tool for gene functional research. It has been clear that the RNAi effect triggered by endogenous or exogenous siRNAs (small interfering RNAs) is transient and dose-dependent. However, there is little information on the regulation of RNAi. Recently, some proteins that regulate the RNA-silencing machinery have been identified. We have observed in previous work that the expression of target genes rebounds after being suppressed for a period of time by siRNAs. In the present study, we used secretory hepatitis B virus surface antigen gene as a reporter and compared its expression level in cell culture and mice challenged by different doses of siRNAs. A quicker and higher rebound of gene expression was observed in mice tail-vein-injected with higher doses of siRNA, and the rebound was associated with an increase in the mRNA level of meri-1 (mouse enhanced RNAi) and adar-1 (adenosine deaminase acting on RNA) genes encoding an exonuclease and RNA-specific adenosine deaminase respectively. Down-regulation of meri-1 by RNAi enhanced the sensitivity and efficiency of siRNA in inhibiting the expression of hepatitis B virus surface antigen. These results indicate that RNAi machinery may be under negative regulation, through the induction of a series of genes coding for destabilizing enzymes, by siRNAs introduced into the cell, and also suggest that a suitable amount of siRNA should be used for research or therapeutic applications.

Cloning a defined region of DNA using a limited action of DNA polymerase: application to dissection of hepatitis B virus surface antigen gene

Gene ◽  
1986 ◽  
Vol 45 (1) ◽  
pp. 19-25
Author(s):  
Chisaka Osamu ◽  
Iwai Shigenori ◽  
Ohtsuka Eiko ◽  
Matsubara Kenichi
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Polymerase ◽  
Surface Antigen ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
Antigen Gene ◽  
B Virus

scholarly journals Overcoming T Cell Tolerance to the Hepatitis B Virus Surface Antigen in Hepatitis B Virus-Transgenic Mice

2001 ◽  
Vol 166 (2) ◽  
pp. 1389-1397 ◽  
Author(s):  
Alessandro D. Sette ◽  
Carla Oseroff ◽  
John Sidney ◽  
Jeff Alexander ◽  
Robert W. Chesnut ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
T Cell ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
T Cell Tolerance ◽  
Cell Tolerance ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
B Virus

scholarly journals Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector

1981 ◽  
Vol 78 (4) ◽  
pp. 2606-2610 ◽  
Author(s):  
A. M. Moriarty ◽  
B. H. Hoyer ◽  
J. W.-k. Shih ◽  
J. L. Gerin ◽  
D. H. Hamer
Keyword(s):  
Cell Culture ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
Antigen Gene ◽  
B Virus

scholarly journals Demethylation by 5-Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice

1989 ◽  
Vol 80 (4) ◽  
pp. 295-298 ◽  
Author(s):  
Kimi Araki ◽  
Jun-ichi Miyazaki ◽  
Toshiki Tsurimoto ◽  
Takeaki Inomoto ◽  
Tomohisa Iwanaga ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
B Virus
Sign in / Sign up

Export Citation Format

Share Document