scholarly journals Predicting Polymer Properties via a Coupled Kinetic, Stochastic and Rheological Modeling Approach from Reaction Conditions

2021 ◽  
pp. 2100027
Author(s):  
Kristina M. Zentel ◽  
Markus Busch
Author(s):  
James F. Hainfeld ◽  
Kyra M. Alford ◽  
Mathias Sprinzl ◽  
Valsan Mandiyan ◽  
Santa J. Tumminia ◽  
...  

The undecagold (Au11) cluster was used to covalently label tRNA molecules at two specific ribonucleotides, one at position 75, and one at position 32 near the anticodon loop. Two different Au11 derivatives were used, one with a monomaleimide and one with a monoiodacetamide to effect efficient reactions.The first tRNA labeled was yeast tRNAphe which had a 2-thiocytidine (s2C) enzymatically introduced at position 75. This was found to react with the iodoacetamide-Aun derivative (Fig. 1) but not the maleimide-Aun (Fig. 2). Reaction conditions were 37° for 16 hours. Addition of dimethylformamide (DMF) up to 70% made no improvement in the labeling yield. A high resolution scanning transmission electron micrograph (STEM) taken using the darkfield elastically scattered electrons is shown in Fig. 3.


2012 ◽  
Author(s):  
Nina R. Arnold ◽  
Ute J. Bayen ◽  
Rebekah E. Smith

2012 ◽  
Author(s):  
Nina R. Arnold ◽  
Ute J. Bayen ◽  
Rebekah E. Smith

2004 ◽  
Author(s):  
Michael D. Byrne ◽  
Alex Kirlik ◽  
Michael D. Fleetwood ◽  
David G. Huss ◽  
Alex Kosorukoff ◽  
...  

2011 ◽  
Author(s):  
Elisa Jayne Bienenstock ◽  
Allison Astorino-Courtois

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