Protein synthesis at synapse versus cell body: Enhanced but transient expression of long-term facilitation at isolated synapses

2003 ◽  
Vol 56 (3) ◽  
pp. 275-286 ◽  
Author(s):  
Ke Liu ◽  
Jiang-Yuan Hu ◽  
Denong Wang ◽  
Samuel Schacher
Keyword(s):  
Protein Synthesis ◽  
Transient Expression ◽  
Cell Body ◽  
Long Term Facilitation

scholarly journals Induction of long-term facilitation in Aplysia sensory neurons by local application of serotonin to remote synapses

1993 ◽  
Vol 90 (23) ◽  
pp. 11411-11415 ◽  
Author(s):  
G A Clark ◽  
E R Kandel
Keyword(s):  
Gene Expression ◽  
Sensory Neurons ◽  
Cell Body ◽  
Sensory Cells ◽  
Target Cells ◽  
Synaptic Terminals ◽  
Dissociated Cell Culture ◽  
Synaptic Changes ◽  
Long Term Facilitation

Long-term synaptic facilitation at the connections of Aplysia sensory neurons onto their target cells involves alterations in gene expression. How then are the relevant cellular signals for the induction and expression of long-term synaptic changes conveyed between the nucleus and remote synaptic terminals? We have explored this question using a set of remote, peripheral terminals of siphon sensory cells, which are approximately 3 cm from the sensory cell body in the abdominal ganglion. We found that these remote synapses, like the proximal synapses previously studied in dissociated cell culture, can exhibit long-term facilitation 24 hr after cell-wide serotonin application. Furthermore, serotonin applications restricted to the remote synaptic terminals nevertheless produced long-term facilitation, indicating that signals generated in synaptic regions can trigger the long-term process, perhaps via retrograde signals to the nucleus to modify gene expression, followed by anterograde signals back to the terminal. Serotonin applications restricted to the cell body and proximal synapses of the sensory neuron also produced long-term facilitation at remote synapses, although to a lesser extent, suggesting that long-term facilitation is expressed cell-wide, but that superimposed on this cell-wide facilitation there appears to be a component that is synapse-specific.

scholarly journals Synapse-Specific, Long-Term Facilitation of Aplysia Sensory to Motor Synapses: A Function for Local Protein Synthesis in Memory Storage

Cell ◽  
1997 ◽  
Vol 91 (7) ◽  
pp. 927-938 ◽  
Author(s):  
Kelsey C Martin ◽  
Andrea Casadio ◽  
Huixiang Zhu ◽  
Yaping E ◽  
Jack C Rose ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Memory Storage ◽  
Local Protein Synthesis ◽  
Long Term Facilitation ◽  
Local Protein

scholarly journals Long-term sensitization training in Aplysia leads to an increase in the expression of BiP, the major protein chaperon of the ER.

1992 ◽  
Vol 119 (5) ◽  
pp. 1069-1076 ◽  
Author(s):  
D Kuhl ◽  
T E Kennedy ◽  
A Barzilai ◽  
E R Kandel
Keyword(s):  
Protein Synthesis ◽  
Motor Neurons ◽  
Structural Changes ◽  
State Level ◽  
Long Term Memory ◽  
Major Protein ◽  
Synaptic Connections ◽  
Term Memory ◽  
Long Term Facilitation

Long-term memory for sensitization of the gill- and siphon-withdrawal reflexes in Aplysia californica requires RNA and protein synthesis. These long-term behavioral changes are accompanied by long-term facilitation of the synaptic connections between the gill and siphon sensory and motor neurons, which are similarly dependent on transcription and translation. In addition to showing an increase in over-all protein synthesis, long-term facilitation is associated with changes in the expression of specific early, intermediate, and late proteins, and with the growth of new synaptic connections between the sensory and motor neurons of the reflex. We previously focused on early proteins and have identified four proteins as members of the immunoglobulin family of cell adhesion molecules related to NCAM and fasciclin II. We have now cloned the cDNA corresponding to one of the late proteins, and identified it as the Aplysia homolog of BiP, an ER resident protein involved in the folding and assembly of secretory and membrane proteins. Behavioral training increases the steady-state level of BiP mRNA in the sensory neurons. The increase in the synthesis of BiP protein is first detected 3 h after the onset of facilitation, when the increase in overall protein synthesis reaches its peak and the formation of new synaptic terminals becomes apparent. These findings suggest that the chaperon function of BiP might serve to fold proteins and assemble protein complexes necessary for the structural changes characteristic of long-term memory.

scholarly journals A Transient, Neuron-Wide Form of CREB-Mediated Long-Term Facilitation Can Be Stabilized at Specific Synapses by Local Protein Synthesis

Cell ◽  
1999 ◽  
Vol 99 (2) ◽  
pp. 221-237 ◽  
Author(s):  
Andrea Casadio ◽  
Kelsey C Martin ◽  
Maurizio Giustetto ◽  
Huixiang Zhu ◽  
Mary Chen ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Local Protein Synthesis ◽  
Long Term Facilitation ◽  
Local Protein

Initiating morphological changes associated with long-term facilitation inAplysia is independent of transcription or translation in the cell body

2005 ◽  
Vol 64 (2) ◽  
pp. 202-212 ◽  
Author(s):  
Peter W. Grabham ◽  
Fang Wu ◽  
Samuel Schacher ◽  
Daniel J. Goldberg
Keyword(s):  
Cell Body ◽  
Morphological Changes ◽  
Long Term Facilitation

scholarly journals A Neuronal Isoform of CPEB Regulates Local Protein Synthesis and Stabilizes Synapse-Specific Long-Term Facilitation in Aplysia

Cell ◽  
2003 ◽  
Vol 115 (7) ◽  
pp. 893-904 ◽  
Author(s):  
Kausik Si ◽  
Maurizio Giustetto ◽  
Amit Etkin ◽  
Ruby Hsu ◽  
Agnieszka M. Janisiewicz ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Local Protein Synthesis ◽  
Long Term Facilitation ◽  
Local Protein

cAMP evokes long-term facilitation in Aplysia sensory neurons that requires new protein synthesis

Science ◽  
1988 ◽  
Vol 240 (4859) ◽  
pp. 1667-1669 ◽  
Author(s):  
S Schacher ◽  
V. Castellucci ◽  
E. Kandel
Keyword(s):  
Protein Synthesis ◽  
Sensory Neurons ◽  
Long Term Facilitation ◽  
New Protein
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