scholarly journals Structural organization of the cytoskeleton in SV40 human corneal epithelial cells cultured on nano- and microscale grooves

Scanning ◽  
2008 ◽  
Vol 30 (5) ◽  
pp. 405-413 ◽  
Author(s):  
Nancy W. Karuri ◽  
Paul F. Nealey ◽  
Christopher J. Murphy ◽  
Ralph M. Albrecht
Keyword(s):  
Epithelial Cells ◽  
Structural Organization ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells ◽  
Cells Cultured

Resveratrol Rescues Human Corneal Epithelial Cells Cultured in Hyperosmolar Conditions

Cornea ◽  
2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Rohit Shetty ◽  
Murali Subramani ◽  
Ponnalagu Murugeswari ◽  
Venkata Ramana Anandula ◽  
Himanshu Matalia ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells ◽  
Cells Cultured

Potential role for laminin 5 in hypoxia-mediated apoptosis of human corneal epithelial cells

2001 ◽  
Vol 114 (22) ◽  
pp. 4033-4040
Author(s):  
Miechia A. Esco ◽  
Zhiyu Wang ◽  
Mark L. McDermott ◽  
Michelle Kurpakus-Wheater
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Mitochondrial Membrane ◽  
Corneal Epithelial Cell ◽  
Corneal Epithelial Cells ◽  
Laminin 5 ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells ◽  
Cells Cultured

Laminin 5 functions to promote cell-matrix adhesion and therefore is hypothesized to abrogate apoptosis initiated through the loss of epithelial cell contact with extracellular matrix. Laminin 5 levels are decreased in epithelial cells cultured in a hypoxic environment. Exposure of epithelial cells to hypoxia may induce apoptotic pathways transmitted through changes in mitochondrial membrane potential. Using an apoptosis assay based on mitochondrial membrane integrity, the effect of hypoxia (2% oxygen) on human corneal epithelial cell viability was determined. Both a virally transformed corneal epithelial cell line and third passage corneal epithelial cells were resistant to hypoxia-mediated apoptosis for up to 5 days in culture. However, at 7 days in culture, a statistically significant increase in apoptosis was noted in hypoxic corneal epithelial cells compared to normoxic (20% oxygen) controls. Increased apoptosis in hypoxic epithelium at 7 days in culture correlated with decreased deposition of laminin 5 into the extracellular matrix, as determined by western blot analysis and immunofluorescence microscopy. Additionally, the extracellular processing of the α3 and γ2 chains of laminin 5 was negatively impacted by corneal epithelial cell exposure to hypoxia for 7 days. Treatment of human corneal epithelial cells cultured in 20% oxygen with function-inhibiting antibodies to laminin 5 for 2 or 3 days resulted in a statistically significant decrease in proliferation, and concomitant increase in apoptosis, compared with untreated normoxic controls. Based on these results, it appears that mechanisms of hypoxia-mediated apoptosis in human corneal epithelial cells may be initiated by the loss of processed laminin 5 in the extracellular matrix or by the loss of laminin 5-epithelial cell communication and transmitted through mitochondria.

Prolonged exposure to high glucose impaired cellular behavior of normal human corneal epithelial cells

2003 ◽  
Vol 27 (4) ◽  
pp. 197-203 ◽  
Author(s):  
Hiroki Fujita ◽  
Ikuo Morita ◽  
Hiroshi Takase ◽  
Kyoko Ohno-Matsui ◽  
Manabu Mochizuki
Keyword(s):  
Epithelial Cells ◽  
High Glucose ◽  
Prolonged Exposure ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Cellular Behavior ◽  
Normal Human ◽  
Human Corneal Epithelial Cells
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