scholarly journals Neuroprotective effects of human amniotic fluid stem cells‐derived secretome in an ischemia/reperfusion model

2020 ◽  
Author(s):  
Vanessa Castelli ◽  
Ivana Antonucci ◽  
Michele d'Angelo ◽  
Alessandra Tessitore ◽  
Veronica Zelli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Ischemia Reperfusion ◽  
Human Amniotic Fluid ◽  
Neuroprotective Effects ◽  
Amniotic Fluid Stem Cells

scholarly journals Amniotic Fluid Cells, Stem Cells, and p53: Can We Stereotype p53 Functions?

2019 ◽  
Vol 20 (9) ◽  
pp. 2236 ◽  
Author(s):  
Melissa Rodrigues ◽  
Christine Blattner ◽  
Liborio Stuppia
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Adult Stem Cells ◽  
Tumor Formation ◽  
Human Stem Cells ◽  
Human Amniotic Fluid ◽  
Amniotic Fluid Stem Cells ◽  
Differentiated Cells ◽  
High Proliferation Rate ◽  
Alternative Sources

In recent years, great interest has been devoted to finding alternative sources for human stem cells which can be easily isolated, ideally without raising ethical objections. These stem cells should furthermore have a high proliferation rate and the ability to differentiate into all three germ layers. Amniotic fluid, ordinarily discarded as medical waste, is potentially such a novel source of stem cells, and these amniotic fluid derived stem cells are currently gaining a lot of attention. However, further information will be required about the properties of these cells before they can be used for therapeutic purposes. For example, the risk of tumor formation after cell transplantation needs to be explored. The tumor suppressor protein p53, well known for its activity in controlling Cell Prolif.eration and cell death in differentiated cells, has more recently been found to be also active in amniotic fluid stem cells. In this review, we summarize the major findings about human amniotic fluid stem cells since their discovery, followed by a brief overview of the important role played by p53 in embryonic and adult stem cells. In addition, we explore what is known about p53 in amniotic fluid stem cells to date, and emphasize the need to investigate its role, particularly in the context of cell tumorigenicity.

291 ISOLATION AND CHARACTERIZATION OF MESENCHYMAL STEM CELLS DERIVED FROM HUMAN AMNIOTIC FLUID

2011 ◽  
Vol 23 (1) ◽  
pp. 243 ◽  
Author(s):  
S.-A. Choi ◽  
J.-H. Lee ◽  
K.-J. Kim ◽  
E.-Y. Kim ◽  
K.-S. Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Amniotic Fluid ◽  
Adult Stem Cells ◽  
Embryonic Stem ◽  
Stem Cell Marker ◽  
Second Trimester ◽  
Cell Marker ◽  
Human Amniotic Fluid ◽  
Amniotic Fluid Stem Cells

Adult stem cells have the capacity to differentiate into several different cell types, although their differentiation potential is limited compared with that of embryonic stem cells. Thus, adult stem cells are regarded as an exciting source for new cell therapies. Recent observations also indicate that stem cells derived from second-trimester amniocentesis are pluripotent – capable of differentiating into multiple lineages, including representatives of all 3 embryonic germ layers. In addition, amniotic fluid stem cells can be used in the generation of disease- or patient-specific stem cells, and amniotic fluid stem cells could be an ideal source for autologous cell replacement therapy in the later life of the fetus. The aim of the present study was to investigate isolation and characterisation of human amniotic fluid-derived mesenchymal stem cells (hAFS). We successfully isolated and characterised hAFS. Amniotic fluid samples were collected in the second trimester (median gestational age: 16 weeks, range: 15–17 weeks) for prenatal diagnosis. Specimens (2 mL) were centrifuged and incubated in low-glucose DMEM supplemented with 10% FBS, 25 ng of basic fibroblast growth factor, and 10 ng of epidermal growth factor at 37°C with 5% CO2. Human amniotic fluid cell (passage 6) expression of stem cell specific markers OCT-4, SOX2, Rex1, FGF4, and NANOG was confirmed by RT-PCR. Flow cytometric analysis showed that hAFS (passage 10) were positive for CD44, CD29, CD146, STRO1, and CD90 but negative for CD19. Immunocytochemical analysis of hAFS (passage 11) also showed the expression of OCT-4, SSEA-1, CD44, CD29, CD146, STRO1, and CD90, but hAFS were negative for CD19 and CD14. In conclusion, according to the previous studies on other mammalians, hAFS are an appropriate source of pluripotent stem cells. Here, we demonstrated that hAFS have a high expression of stem cell specific marker, including embryonic stem cell marker and mesenchymal stem cell marker. Therefore, amniotic fluid may be a suitable alternative source of multipotent stem cells.

scholarly journals Intravenous Grafts of Human Amniotic Fluid-Derived Stem Cells Reduce Behavioral Deficits in Experimental Ischemic Stroke

2019 ◽  
Vol 28 (9-10) ◽  
pp. 1306-1320 ◽  
Author(s):  
Tatiana Taís Sibov ◽  
Lorena Favaro Pavon ◽  
Francisco Romero Cabral ◽  
Ivone Farias Cunha ◽  
Daniela Mara de Oliveira ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Cellular Therapy ◽  
Ischemia Reperfusion Injury ◽  
Focal Cerebral Ischemia ◽  
Infarct Volume ◽  
Ischemia Reperfusion ◽  
Cerebral Injury ◽  
Human Amniotic Fluid ◽  
Severity Scores

Amniotic fluid has been investigated as new cell source for stem cells in the development of future cell-based transplantation. This study reports isolation of viable human amniotic fluid-derived stem cells, labeled with multimodal iron oxide nanoparticles, and its effect on focal cerebral ischemia–reperfusion injury in Wistar rats. Middle cerebral artery occlusion of 60 min followed by reperfusion for 1 h, 6 h, and 24 h was employed in the present study to produce ischemia and reperfusion-induced cerebral injury in rats. Tests were employed to assess the functional outcome of the sensorimotor center activity in the brain, through a set of modified neurological severity scores used to assess motor and exploratory capacity 24 h, 14, and 28 days after receiving cellular therapy via tail vein. In our animal model of stroke, transplanted cells migrated to the ischemic focus, infarct volume decreased, and motor deficits improved. Therefore, we concluded that these cells appear to have beneficial effects on the ischemic brain, possibly based on their ability to enhance endogenous repair mechanisms.

Renal tubular regeneration from human Amniotic Fluid Stem Cells

2005 ◽  
Vol 201 (3) ◽  
pp. S47 ◽  
Author(s):  
Roger De Filippo ◽  
Laura Perin ◽  
Stefano Giuliani ◽  
Akito Maeshima ◽  
Daniel Jin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Human Amniotic Fluid ◽  
Amniotic Fluid Stem Cells ◽  
Renal Tubular

scholarly journals Human Amniotic Fluid Stem Cells: Therapeutic Potential for Perinatal Patients with Intractable Neurological Disease

2018 ◽  
Vol 67 (4) ◽  
pp. 57-66 ◽  
Author(s):  
Daigo Ochiai ◽  
Hirotaka Masuda ◽  
Yushi Abe ◽  
Toshimitsu Otani ◽  
Marie Fukutake ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Neurological Disease ◽  
Therapeutic Potential ◽  
Human Amniotic Fluid ◽  
Amniotic Fluid Stem Cells

scholarly journals Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Tullia Maraldi ◽  
Marianna Guida ◽  
Manuela Zavatti ◽  
Elisa Resca ◽  
Laura Bertoni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Amniotic Fluid ◽  
Human Stem Cells ◽  
Human Amniotic Fluid ◽  
Differentiation Potential ◽  
Amniotic Fluid Stem Cells ◽  
Target Molecules ◽  
Cellular Compartments

Human amniotic fluid stem cells (AFSC) are an attractive source for cell therapy due to their multilineage differentiation potential and accessibility advantages. However the clinical application of human stem cells largely depends on their capacity to expandin vitro, since there is an extensive donor-to-donor heterogeneity. Reactive oxygen species (ROS) and cellular oxidative stress are involved in many physiological and pathophysiological processes of stem cells, including pluripotency, proliferation, differentiation, and stress resistance. The mode of action of ROS is also dependent on the localization of their target molecules. Thus, the modifications induced by ROS can be separated depending on the cellular compartments they affect. NAD(P)H oxidase family, particularly Nox4, has been known to produce ROS in the nucleus. In the present study we show that Nox4 nuclear expression (nNox4) depends on the donor and it correlates with the expression of transcription factors involved in stemness regulation, such as Oct4, SSEA-4, and Sox2. Moreover nNox4 is linked with the nuclear localization of redox sensitive transcription factors, as Nrf2 and NF-κB, and with the differentiation potential. Taken together, these results suggest that nNox4 regulation may have important effects in stem cell capability through modulation of transcription factors and DNA damage.

scholarly journals The neurorestorative effect of human amniotic fluid stem cells on the chronic phase of neonatal hypoxic–ischemic encephalopathy in mice

2018 ◽  
Vol 85 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Toshimitsu Otani ◽  
Daigo Ochiai ◽  
Hirotaka Masuda ◽  
Yushi Abe ◽  
Marie Fukutake ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Chronic Phase ◽  
Hypoxic Ischemic Encephalopathy ◽  
Human Amniotic Fluid ◽  
Amniotic Fluid Stem Cells ◽  
Ischemic Encephalopathy

scholarly journals Human Amniotic Fluid Stem Cells as an Attractive Tool for Clinical Applications

2013 ◽  
Vol 8 (2) ◽  
pp. 125-132 ◽  
Author(s):  
Ourania Trohatou ◽  
Nicholas P. Anagnou ◽  
Maria G. Roubelakis
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Clinical Applications ◽  
Human Amniotic Fluid ◽  
Amniotic Fluid Stem Cells
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