Mechanism of Hydrolysis of the Treated Sago Starch Granules by Raw Starch Digesting Amylase fromPenicillium brunneum

1992 ◽  
Vol 44 (1) ◽  
pp. 25-28 ◽  
Author(s):  
Nadirman Haska ◽  
Yoshiyuki Ohta
1962 ◽  
Vol 40 (12) ◽  
pp. 1659-1673 ◽  
Author(s):  
G. M. Simpson ◽  
J. M. Naylor

Initiation of germination in excised embryos requires an exogenous energy source. Normally this is obtained from the endosperm. In dormant seeds the hydrolysis of starch is blocked despite the fact that dormant and non-dormant seeds contain similar amounts of α- and β-amylases. Alone or in combination the amylases are unable to break down raw endosperm starch granules to simple sugars in vitro. Exogenous maltase in combination with α-amylase hydrolyzes raw starch to glucose. Exogenous maltase eliminates the requirement for exogenous sugar. Examination of the maltase content of imbibed dormant and non-dormant seeds showed a marked increase in non-dormant seeds during the first 40 hours. This does not occur in dormant seeds unless they are treated with gibberellic acid. The results lead to the conclusion that an important effect of gibberellic acid is to induce the synthesis of maltase or in some way activate the preformed enzyme.


1997 ◽  
Vol 33 (2-3) ◽  
pp. 195-202 ◽  
Author(s):  
W.J. Wang ◽  
A.D. Powell ◽  
C.G. Oates

1966 ◽  
Vol 5 (11) ◽  
pp. 1943-1946 ◽  
Author(s):  
Franklin H. Fry ◽  
Gordon A. Hamilton ◽  
John Turkevich

1982 ◽  
Vol 35 (7) ◽  
pp. 1357 ◽  
Author(s):  
TJ Broxton

The hydrolysis of 2-acetyloxybenzoic acid in the pH range 6-12 has been studied in the presence of micelles of cetyltrimethylammonium bromide (ctab) and cetylpyridinium chloride (cpc). In the plateau region (pH 6-8) the hydrolysis is inhibited by the presence of micelles, while in the region where the normal BAC2 hydrolysis (pH > 9) occurs the reaction is catalysed by micelles of ctab and cpc. The mechanism of hydrolysis in the plateau region is shown to involve general base catalysis by the adjacent ionized carboxy group both in the presence and absence of micelles. This reaction is inhibited in the presence of micelles because the substrate molecules are solubilized into the micelle and water is less available in this environment than in normal aqueous solution.


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