Adenosine Triphosphate Prevents Serum Deprivation-Induced Apoptosis in Human Mesenchymal Stem Cells via Activation of the MAPK Signaling Pathways

The regeneration of bone and tooth tissues, and related cellular therapies, has attracted widespread attention. Bone marrow mesenchymal stem cells (BMSCs) are potential candidates for such regeneration. iRoot SP is a premixed bioceramic root canal sealer widely used in clinical settings. However, the effect of iRoot SP on the biological features of BMSCs has not been elucidated. In the present study, we found that 0.2 mg/ml iRoot SP conditioned medium promoted osteo/odontogenic differentiation and enhanced mineralization of BMSCs without affecting the proliferative ability. Mechanistically, the NF-κB and MAPK signaling pathways were activated in SP-treated BMSCs, and differentiation was inhibited when cultured with the specific inhibitor. Taken together, these findings demonstrate that iRoot SP promotes osteo/odontogenic differentiation of BMSCs via the NF-κB and MAPK signaling pathways, which could provide a new theoretical basis for clinical applications of iRoot SP and a new therapeutic target for the regeneration of bone and tooth tissue in the future.

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Roles of calcium phosphate-mediated integrin expression and MAPK signaling pathways in the osteoblastic differentiation of mesenchymal stem cells

Journal of Materials Chemistry B ◽

10.1039/c6tb00349d ◽

2016 ◽

Vol 4 (13) ◽

pp. 2280-2289 ◽

Cited By ~ 38

Author(s):

Xuening Chen ◽

Jing Wang ◽

Ying Chen ◽

Hanxu Cai ◽

Xiao Yang ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Calcium Phosphate ◽

Signaling Pathways ◽

Osteoblastic Differentiation ◽

Mapk Signaling ◽

Integrin Expression ◽

Signaling Transduction ◽

Mapk Signaling Pathways

BCP ceramics mediated MSC's integrin expression to realize “outside-in signaling” transduction and then activated MAPK signaling to induce osteogenesis.

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Could the effect of modeled microgravity on osteogenic differentiation of human mesenchymal stem cells be reversed by regulation of signaling pathways?

Biological Chemistry ◽

10.1515/bc.2007.082 ◽

2007 ◽

Vol 388 (7) ◽

Cited By ~ 25

Author(s):

Qiang Zheng ◽

Guoping Huang ◽

Jinfeng Yang ◽

Yulin Xu ◽

Chunjuan Guo ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Signaling Pathways ◽

Human Mesenchymal Stem Cells ◽

Modeled Microgravity

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Migration of Human Mesenchymal Stem Cells is Stimulated by Low Shear Stress via MAPK Signaling

ASME 2012 Summer Bioengineering Conference, Parts A and B ◽

10.1115/sbc2012-80056 ◽

2012 ◽

Author(s):

Lin Yuan ◽

Naoya Sakamoto ◽

Guanbin Song ◽

Masaaki Sato

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Human Mesenchymal Stem Cells ◽

Mapk Signaling ◽

Disease Treatment ◽

Differentiation Potential ◽

Multipotent Stem Cells ◽

Low Shear Stress ◽

Multipotent Differentiation ◽

And Migration

Mesenchymal stem cells (MSCs) represent as multipotent stem cells which hold the abilities of self-renewal and give rise to cells of diverse lineages [1]. With their remarkable combination of multipotent differentiation potential and low immunogenicity, MSCs are considered to be an attractive candidate for cell-based tissue repair and regenerative tissue engineering [2, 3]. Increasing number of studies has demonstrated that mobilization and migration of injected MSCs to the damaged tissues is a key step for these cells to participate in disease treatment and tissue regeneration [4, 5].

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