Ethylenethiourea-induced hydrocephalus in vivo and in vitro with a note on the use of a constant gaseous atmosphere for rat embryo cultures

Teratology ◽  
1989 ◽  
Vol 39 (3) ◽  
pp. 277-285 ◽  
Author(s):  
K. S. Khera
Keyword(s):  
Gaseous Atmosphere ◽  
Rat Embryo ◽  
Embryo Cultures

Cadherin mRNAs during rat embryo development in vivo and in vitro

Teratology ◽  
1991 ◽  
Vol 44 (5) ◽  
pp. 581-590 ◽  
Author(s):  
Beiyun Chen ◽  
Orest W. Blaschuk ◽  
Barbara F. Hales
Keyword(s):  
Embryo Development ◽  
Rat Embryo

scholarly journals Physical and Functional Interactions between the Corepressor CtBP and the Epstein-Barr Virus Nuclear Antigen EBNA3C

2001 ◽  
Vol 75 (16) ◽  
pp. 7749-7755 ◽  
Author(s):  
Robert Touitou ◽  
Mark Hickabottom ◽  
Gillian Parker ◽  
Tim Crook ◽  
Martin J. Allday
Keyword(s):  
Epstein Barr Virus ◽  
Nuclear Antigen ◽  
Rat Embryo ◽  
Barr Virus ◽  
Functional Interactions ◽  
C Terminus ◽  
Epstein Barr ◽  
Repressor Activity

ABSTRACT CtBP has been shown to be a highly conserved corepressor of transcription. E1A and all the various transcription factors to which CtBP binds contain a conserved PLDLS CtBP-interacting domain, and EBNA3C includes a PLDLS motif (amino acids [aa] 728 to 732). Here we show that EBNA3C binds to CtBP both in vitro and in vivo and that the interaction requires an intact PLDLS. The C terminus of EBNA3C (aa 580 to 992) has modest trans-repressor activity when it is fused to the DNA-binding domain of Gal4, and deletion or mutation of the PLDLS sequence ablates this and unmasks a transactivation function within the fragment. However, loss of the CtBP interaction motif had little effect on the ability of full-length EBNA3C to repress transcription. A striking correlation between CtBP binding and the capacity of EBNA3C to cooperate with (Ha-)Ras in the immortalization and transformation of primary rat embryo fibroblasts was also revealed.

In vivo and in vitro differentiation of neurons and astrocytes in the rat embryo

1981 ◽  
Vol 85 (2) ◽  
pp. 344-357 ◽  
Author(s):  
Trichur Raju ◽  
Amico Bignami ◽  
Doris Dahl
Keyword(s):  
In Vitro Differentiation ◽  
Rat Embryo

Rat Embryo Cultures for In Vitro Teratology

2000 ◽  
Vol 6 (1) ◽  
Author(s):  
Alan G. Fantel ◽  
Rudolf Bechter ◽  
David Beckman
Keyword(s):  
Rat Embryo ◽  
Embryo Cultures

The effect of trypan blue on the growth of the rat embryo in vivo

Development ◽  
1970 ◽  
Vol 23 (1) ◽  
pp. 213-218
Author(s):  
Colin L. Berry
Keyword(s):  
Growth Rate ◽  
Chick Embryo ◽  
Protein Content ◽  
Trypan Blue ◽  
In Vitro Growth ◽  
Rat Embryo ◽  
Somite Formation ◽  
Somite Number

In a previous paper (Berry, 1968) it has been demonstrated, by comparison of in vivo and in vitro growth of the rat foetus, that it is possible to dissociate growth and differentiation in the rat. In a series now exceeding 1000 embryos it is evident that for a given somite number the protein content of an animal developing in vitro might be less than half that of the normal control, suggesting that a considerable reduction in growth rate might be found without necessarily inducing death or malformation. The rate of somite formation is not reduced in vitro in the rat; it has been shown by Herrman & Schultz (1958) that the rate of somite formation is not interfered with by different expiant conditions in the chick embryo. These findings suggest that the increase in somite number is more rigidly determined than the increase in growth rate.

scholarly journals THE DEVELOPMENT OF THE DORSAL AND VENTRAL MAMMALIAN PANCREAS IN VIVO AND IN VITRO

1970 ◽  
Vol 47 (1) ◽  
pp. 235-246 ◽  
Author(s):  
Brian S. Spooner ◽  
Bernt T. Walther ◽  
William J. Rutter
Keyword(s):  
Culture System ◽  
Specific Activity ◽  
Rat Embryo ◽  
Dorsal Pancreas ◽  
Somite Stage ◽  
Ventral Pancreas ◽  
The Times ◽  
Biochemical Differentiation

The origin, morphogenesis, and biochemical differentiation of the dorsal and ventral pancreas of the rat embryo have been investigated in order to ascertain the similarities and dissimilarities between the two lobes. We have utilized a culture system in which the primitive gut gives rise to a number of differentiated organs, including the dorsal and ventral pancreas. The two pancreases do not undergo fusion in these cultures, thus allowing independent analyses of the two lobes for comparison with in vivo results. The dorsal pancreas first appeared at the 23–25 somite stage while the ventral pancreas appeared approximately 12 hr later at the 29–30 somite stage. Guts from embryos as young as 12 somites were capable of developing both pancreases in vitro. In spite of the 12 hr difference between the times of their appearance, the dorsal and ventral pancreases exhibited identical patterns of morphological and biochemical differentiation. The two lobes contained the same exocrine enzymes and hormones, at similar levels, differing only in their glucagon content, the dorsal pancreas possessing a fivefold higher glucagon specific activity. The implications of these results are discussed.

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