scholarly journals Characterization of a temperature-sensitive mutation that impairs the function of yeast tRNA nucleotidyltransferase

Yeast ◽  
2008 ◽  
Vol 25 (3) ◽  
pp. 219-233 ◽  
Author(s):  
Xunying Shan ◽  
Tara A. Russell ◽  
Scott M. Paul ◽  
David B. Kushner ◽  
Paul B. M. Joyce
Keyword(s):  
Temperature Sensitive ◽  
Yeast Trna ◽  
Temperature Sensitive Mutation

Temperature-sensitive mutation. IV. Induction and characterization of a ts-mutation in RNA-containing bacteriophage R17

1970 ◽  
Vol 16 (3) ◽  
pp. 165-172
Author(s):  
S. J. Igarashi ◽  
J. F. Elliott ◽  
R. P. Bissonnette
Keyword(s):  
Rna Replication ◽  
Viral Rna ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Temperature Sensitive Mutants ◽  
Temperature Sensitive Mutation ◽  
Phage Multiplication ◽  
Rna Phage

Temperature-sensitive mutants of the RNA phage R17 were induced by treatment with 10−5 M 5-fluorouracil. One of the temperature-sensitive mutants, ts24, was studied and the following facts were discovered: (1) ts24 undergoes eclipse at the non-permissive temperature, 43 °C. (2) If the temperature is increased to non-permissive levels before 60 min of postinfection culture, phage multiplication ceases. (3) At 43 °C, ts24 cannot synthesize progeny viral RNA. (4) Upon decreasing the temperature to permissive levels during the first 60 min of postinfection culture, RNA replication resumes almost instantaneously. It was concluded from these facts that ts24 contains a temperature-sensitive mutation in the RNA replicating function.

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